Dopamine D3 Receptor mRNA and Renal Response to D3 Receptor Activation in Spontaneously Hypertensive Rats

Luippold, Gerd; Piesch, Christine; Oßwald, Hartmut; Mühlbauer, Bernd

doi:10.1291/hypres.26.855

Cited by 10 publications

(9 citation statements)

References 24 publications

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“…This effect is confirmed in salt-sensitive Dahl rats, WKY rats and SHRs [41,42] , which may contribute the inhibitory effect of D 3 receptor on Na + -K + -ATPase activity in RPTs [43,44] . Our present study found that stimulation of D 3 receptor inhibits Na + -K + -ATPase activity in RPT cells.…”

Section: Discussionmentioning

confidence: 79%

Impaired Stimulatory Effect of ETB Receptor on D<sub>3</sub> Receptor in Immortalized Renal Proximal Tubule Cells of Spontaneously Hypertensive Rats

Zhang

Fu²,

Ren³

et al. 2011

Kidney Blood Press Res

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Background: Activation of renal D₃ receptor induces natriuresis and diuresis in Wistar-Kyoto (WKY) rats; in the presence of ETB receptor antagonist, the natriuretic effect of D₃ receptor in WKY rats is reduced. We hypothesize that ETB receptor activation may regulate D₃ receptor expression in renal proximal tubule (RPT) cells from WKY rats, which is impaired in RPT cells from spontaneously hypertensive rats (SHRs). Methods: D₃ receptor expression was determined by immunoblotting; the D₃/ETB receptor linkage was checked by coimmunoprecipitation; Na⁺-K⁺-ATPase activity was determined as the rate of inorganic phosphate released in the presence or absence of ouabain. Results: In RPT cells from WKY rats, the ETB receptor agonist BQ3020 increased D₃ receptor protein. In contrast, in RPT cells from SHRs, BQ3020 did not increase D₃ receptor. There was coimmunoprecipitation between D₃ and ETB receptors in RPT cells from WKY and SHRs. Activation of ETB receptor increased D₃/ETB coimmunoprecipitation in RPT cells from WKY rats, but not from SHRs. The basal levels of D₃/ETB receptor coimmunoprecipitation were greater in RPT cells from WKY rats than in those from SHRs. Stimulation of D₃ receptor inhibited Na⁺-K⁺-ATPase activity, which was augmented by the pretreatment with the ETB receptor agonist BQ3020 in WKY RPT cells, but not in SHR RPT cells. Conclusion: ETB receptors regulate and physically interact with D₃ receptors differently in WKY rats and SHRs. The impaired natriuretic effect in SHRs may be, in part, related to impaired ETB and D₃ receptor interactions.

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Section: Discussionmentioning

confidence: 79%

Impaired Stimulatory Effect of ETB Receptor on D<sub>3</sub> Receptor in Immortalized Renal Proximal Tubule Cells of Spontaneously Hypertensive Rats

Zhang

Fu²,

Ren³

et al. 2011

Kidney Blood Press Res

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“…3,47 In contrast, these investigators did not find a defective response to the intravenous infusion of a D 3 receptor agonist [R(ϩ)-7-hydroxydipropylaminotetralin] in SHRs. 48 However, the nonrenal systemic effects of D 3 receptor stimulation may have obfuscated any potential differences between WKY and SHRs. The studies of Luippold et al 47 in SHRs were also not performed in salt-loaded rats; moderate salt loading enhances the natriuretic effects of dopaminergic drugs.…”

Section: Discussionmentioning

confidence: 99%

D ₃ Dopamine Receptor Directly Interacts With D ₁ Dopamine Receptor in Immortalized Renal Proximal Tubule Cells

Zeng

Wang

et al. 2006

Hypertension

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Abstract-D 3 receptors act synergistically with D 1 receptors to inhibit sodium transport in renal proximal tubules; however, the mechanism by which this occurs is not known. The increase in sodium excretion after a sodium load is regulated, in part, by renal paracrine activation of D 1 -like receptors. 1-4 However, D 2 -like receptors may act, synergistically, with D 1 -like receptors to increase urinary sodium excretion. 5,6 Thus, we found that the increase in sodium excretion induced by Z-1046, a dopamine receptor agonist with the rank order potency D 3 ՆD 4 ϾD 2 ϾD 5 ϾD 1 , was blocked by either a D 1 -like or D 2 -like receptor antagonist. 5,6 Several studies have demonstrated that activation of D 1 -like receptors in renal proximal tubules (RPTs) decreases sodium reabsorption by inhibition of the activities of Na ϩ -H ϩ exchanger type 3 (NHE3), Cl Ϫ /HCO 3 Ϫ exchanger, Na/Pi cotransporter in brush border membranes, and Na ϩ /HCO 3 Ϫ cotransporter and Na ϩ -K ϩ -ATPase activities in basolateral membranes. 7-11 D 2 -like receptors may potentiate the inhibitory effect of D 1 -like receptors on Na-Pi cotransporter, NHE3, and Na ϩ -K ϩ -ATPase activities in RPTs. 5,7,9,12 In the rat kidney, the major D 2 -like receptor in RPTs is the D 3 receptor. 1,3,13,14 The D 2 receptor in the rat kidney appears to be located prejunctionally in dopaminergic nerves, 15-17 whereas the D 4 receptor is mainly expressed in collecting ducts 18 and the S 1 segment of the proximal tubule. 16 However, both D 2 and D 4 receptors have been described in the opossum kidney cell, a proximal tubule cell line that has some distal tubular cell characteristics. 19 The major D 1 -like receptor in RPTs is probably the D 1 rather than the D 5 receptor.

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“…The diminished functional response to D 3 receptor stimulation in the hypertensive DS rat is associated with decreased 3 [H]-7-OH-DPAT binding to renal membrane protein [50]. However, the same group did not find a strain-dependent effect of 7-OH-DAT in WKY and SHRs [59]. The interpretation of this study is confounded by the systemic administration of 7-OH-DPAT; activation of D 3 receptors outside the kidney makes interpretation difficult.…”

Section: Role Of D 3 Receptors In Hypertensionmentioning

confidence: 99%

D3 Dopamine Receptor and Essential Hypertension

Zeng¹,

Eisner²,

Felder³

et al. 2006

CHYR

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Dopamine receptors are expressed in a number of organs and tissues; the peripheral dopamine receptors influence cardiovascular and renal function by decreasing preload and afterload and by regulating fluid and electrolyte transport. Most of the knowledge on these actions of dopamine has been garnered from studies of D 1 -like dopamine receptors. The D 3 dopamine receptor subtype, which belongs to the D 2 -like receptor subfamily, has been extensively studied in the neurosciences. Recently, the peripheral actions of the D 3 receptor have also raised considerable interest. Previous studies showed that the D 3 receptor is expressed in organs outside the central nervous system such as the kidneys and blood vessels. Activation of the D 3 receptor, alone or in synergism with D 1 receptor induces diuresis and natriuresis and dilates resistance vessels. The D 3 receptor also interacts positively with endothelin type B receptors and negatively with the renin-angiotensin-aldosterone system. For example, the D 3 receptor-mediated natriuresis can be blocked by ETB receptor antagonists and stimulation of the D 3 receptor decreases AT 1 receptor expression in renal proximal tubule cells and inhibits renin and aldosterone secretion. The high blood pressure observed in spontaneously hypertensive rats and D 3 receptor deficient mice, may, in part, be caused by impaired D 3 receptor-mediated renal sodium excretion, impaired interaction with D 1 and ETB receptors and impaired inhibition of the renin-angiotensin-aldosterone system. In this review, we examine the role of the peripheral D 3 receptor in the regulation of renal sodium excretion and vascular resistance, and its interaction with the renin-angiotensin system and other dopamine receptor subtypes. We also present our current understanding of the role of D 3 receptor in the pathophysiology of human essential hypertension.

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Dopamine D3 Receptor mRNA and Renal Response to D3 Receptor Activation in Spontaneously Hypertensive Rats

Cited by 10 publications

References 24 publications

Impaired Stimulatory Effect of ETB Receptor on D<sub>3</sub> Receptor in Immortalized Renal Proximal Tubule Cells of Spontaneously Hypertensive Rats

Impaired Stimulatory Effect of ETB Receptor on D<sub>3</sub> Receptor in Immortalized Renal Proximal Tubule Cells of Spontaneously Hypertensive Rats

D ₃ Dopamine Receptor Directly Interacts With D ₁ Dopamine Receptor in Immortalized Renal Proximal Tubule Cells

D3 Dopamine Receptor and Essential Hypertension

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Dopamine D3 Receptor mRNA and Renal Response to D3 Receptor Activation in Spontaneously Hypertensive Rats

Cited by 10 publications

References 24 publications

Impaired Stimulatory Effect of ETB Receptor on D<sub>3</sub> Receptor in Immortalized Renal Proximal Tubule Cells of Spontaneously Hypertensive Rats

Impaired Stimulatory Effect of ETB Receptor on D<sub>3</sub> Receptor in Immortalized Renal Proximal Tubule Cells of Spontaneously Hypertensive Rats

D 3 Dopamine Receptor Directly Interacts With D 1 Dopamine Receptor in Immortalized Renal Proximal Tubule Cells

D3 Dopamine Receptor and Essential Hypertension

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D ₃ Dopamine Receptor Directly Interacts With D ₁ Dopamine Receptor in Immortalized Renal Proximal Tubule Cells