Dopamine Receptors Gene Expression Pattern and Locomotor Improvement Differ Between Female and Male Zebrafish During Spinal Cord Auto Repair

Hosseini, Parastoo; Mirsadeghi, Sara; Rahmani, Saeid; Izadi, Azam; Rezaei, Mohammad; Ghodsi, Zahra; Rahimi‐Movaghar, Vafa; Kiani, Sahar

doi:10.1089/zeb.2021.0081

“…We noted that male fish exhibited this stereotypical recovery trend more strongly than females in this experiment (Figure S1A-F). Overall, the female fish in this experiment regenerated poorly (Figure S1F) and were less energetic both before and after injury, which is not necessarily typical of zebrafish (14). For this reason, female fish data are presented in the supplement.…”

Section: A Longitudinal Study Of Spinal Cord Regeneration Parametersmentioning

confidence: 91%

“…Since 2020, the toolkit to quantify swim function has rapidly expanded and diversified. Traditional and recently established measurements of swim capacity include swim endurance as quantified by time to exhaustion against increasing water current velocities (2,6,8,9,10), maximum speed either against increasing water current velocities or in a static tank (11,12,13,14), swim distance (7,9), time active (9,10), time swimming against flow (9), burst frequency (9,10), and average position of the fish against a water flow axis ("mean y") (9,10). Although new quality measurements have been developed for larval fish including body angle (15), power spectral densities (16), and centerline posture kinematics (17), published quality measurements for adult fish are limited to manual scoring of perceived swim ability (3,4,5), tail beat frequency and amplitude (12).…”

Section: Introductionmentioning

confidence: 99%

Functional Trajectories during innate spinal cord repair

Jensen

¹

,

Burris

²

,

Zhou

³

et al. 2023

Preprint

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Adult zebrafish are capable of anatomical and functional recovery following severe spinal cord injury. Axon growth, glial bridging and adult neurogenesis are hallmarks of cellular regeneration during spinal cord repair. However, the correlation between these cellular regenerative processes and functional recovery remains to be elucidated. Whereas the majority of established functional regeneration metrics measure swim capacity, we hypothesize that gait quality is more directly related to neurological health. Here, we performed a longitudinal swim tracking study for sixty individual zebrafish spanning eight weeks of spinal cord regeneration. Multiple swim parameters as well as axonal and glial bridging were integrated. We established rostral compensation as a new gait quality metric that highly correlates with functional recovery. Tensor component analysis of longitudinal data supports a correspondence between functional recovery trajectories and neurological outcomes. Moreover, our studies predicted and validated that a subset of functional regeneration parameters measured 1 to 2 weeks post-injury is sufficient to predict the regenerative outcomes of individual animals at 8 weeks post-injury. Our findings established new functional regeneration parameters and generated a comprehensive correlative database between various functional and cellular regeneration outputs.

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“…We noted that male fish exhibited this stereotypical recovery trend more strongly than females in this experiment (Supplementary Figures S1A-F). Overall, the female fish in this experiment regenerated poorly (Supplementary Figure S1F) and were less energetic both before and after injury, which is not necessarily typical of zebrafish (Hosseini et al, 2022). For this reason, female fish data are presented in the supplement.…”

Section: A Longitudinal Study Of Spinal Cord Regeneration Parametersmentioning

confidence: 94%

“…Since 2020, the toolkit to quantify swim function has rapidly expanded and diversified. Traditional and recently established measurements of swim capacity include swim endurance as quantified by time to exhaustion against increasing water current velocities (van Raamsdonk et al, 1998;Mokalled et al, 2016;Burris et al, 2021;Klatt Shaw and Mokalled, 2021;Saraswathy et al, 2022), maximum speed either against increasing water current velocities or in a static tank (Chang et al, 2021;Huang et al, 2021;Hosseini et al, 2022;Huang et al, 2022), swim distance (Becker et al, 2004;Burris et al, 2021), time active (Burris et al, 2021;Saraswathy et al, 2022), time swimming against flow (Burris et al, 2021), burst frequency (Burris et al, 2021;Saraswathy et al, 2022), and average position of the fish against a water flow axis ("mean y") (Burris et al, 2021;Saraswathy et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

“…Since 2020, the toolkit to quantify swim function has rapidly expanded and diversified. Traditional and recently established measurements of swim capacity include swim endurance as quantified by time to exhaustion against increasing water current velocities ( van Raamsdonk et al, 1998 ; Mokalled et al, 2016 ; Burris et al, 2021 ; Klatt Shaw and Mokalled, 2021 ; Saraswathy et al, 2022 ), maximum speed either against increasing water current velocities or in a static tank ( Chang et al, 2021 ; Huang et al, 2021 ; Hosseini et al, 2022 ; Huang et al, 2022 ), swim distance ( Becker et al, 2004 ; Burris et al, 2021 ), time active ( Burris et al, 2021 ; Saraswathy et al, 2022 ), time swimming against flow ( Burris et al, 2021 ), burst frequency ( Burris et al, 2021 ; Saraswathy et al, 2022 ), and average position of the fish against a water flow axis (“mean y”) ( Burris et al, 2021 ; Saraswathy et al, 2022 ). Although new quality measurements have been developed for larval fish including body angle ( Zeng et al, 2021 ), power spectral densities ( Vasudevan et al, 2021 ), and centerline posture kinematics ( Hossainian et al, 2022 ), published quality measurements for adult fish are limited to manual scoring of perceived swim ability ( Becker et al, 1997 ; Goldshmit et al, 2012 ; Strand et al, 2016 ), tail beat frequency and amplitude ( Huang et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Functional trajectories during innate spinal cord repair

Jensen

¹

,

Burris

²

,

Zhou

³

et al. 2023

Front. Mol. Neurosci.

View full text Add to dashboard Cite

Adult zebrafish are capable of anatomical and functional recovery following severe spinal cord injury. Axon growth, glial bridging and adult neurogenesis are hallmarks of cellular regeneration during spinal cord repair. However, the correlation between these cellular regenerative processes and functional recovery remains to be elucidated. Whereas the majority of established functional regeneration metrics measure swim capacity, we hypothesize that gait quality is more directly related to neurological health. Here, we performed a longitudinal swim tracking study for 60 individual zebrafish spanning 8 weeks of spinal cord regeneration. Multiple swim parameters as well as axonal and glial bridging were integrated. We established rostral compensation as a new gait quality metric that highly correlates with functional recovery. Tensor component analysis of longitudinal data supports a correspondence between functional recovery trajectories and neurological outcomes. Moreover, our studies predicted and validated that a subset of functional regeneration parameters measured 1 to 2 weeks post-injury is sufficient to predict the regenerative outcomes of individual animals at 8 weeks post-injury. Our findings established new functional regeneration parameters and generated a comprehensive correlative database between various functional and cellular regeneration outputs.

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Effect of <i>Passiflora incarnata</i> L. extract on exploratory behaviour and neurotransmitters level in structures involved in motor functions in rats

Blecharz-Klin,

Pyrzanowska,

Piechal

et al. 2024

J Pre Clin Clin Res.

1

0

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Introduction andObjective. The genus Passiflora incarnata L. is used in traditional medicine for its analgesic, anxiolytic and sedative purposes, and its flavoursome fruits have long been consumed, especially in North America. The aim of the study is to assess the effect of extract of P. incarnata L. on the motor activity of rats. Materials and Method. Behavioural testing was performed in rats previously exposed to P. incarnata L. extract (30, 100, 300 mg/kg b.w). To explain the neurobiochemical background of behavioural changes, the concentration of neurotransmitters after extract consumption were assayed in CNS structures responsible for motor function using High Performance Liquid Chromatography.Results. The study demonstrated that exposure to the extract resulted in dose dependent modulation of rats behaviour, and altered neurotransmission in major elements of the CNS locomotor system. The changes concerned primarily the cerebellar and spinal dopaminergic and noradrenergic systems. Conclusions. Inhibitory effect of P. incarnata L. extract on the GABAergic pathway has potential as a sedative agent and may be promising alternative to benzodiazepines in controlling anxiety and hyperactivity. The significant increase in the level of spinal dopamine may be potentially useful in disorders related to a deficit in dopaminergic neurotransmission. These findings could lead to the development of specific supportive therapies for anxiety and symptoms of gradual loss or impairment of motor behaviour.

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Dopamine Receptors Gene Expression Pattern and Locomotor Improvement Differ Between Female and Male Zebrafish During Spinal Cord Auto Repair

Cited by 4 publications

References 58 publications

Functional Trajectories during innate spinal cord repair

Functional Trajectories during innate spinal cord repair

Functional trajectories during innate spinal cord repair

Effect of <i>Passiflora incarnata</i> L. extract on exploratory behaviour and neurotransmitters level in structures involved in motor functions in rats

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