Dopamine Synaptic Mechanisms Reflected in Studies Combining Behavioural Recordings and Brain Dialysis

Ungerstedt, Urban; Herrera‐Marschitz, Mario; Jungnelius, Ulf; Ståhle, Lars; Tossman, U.; Zetterström, T.

doi:10.1016/b978-0-08-027391-4.50025-3

Cited by 142 publications

(74 citation statements)

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“…Voltammetry in vivo will possibly prove of most use in the study of 5-hydroxytryptamine metabolism, which is relatively simple, rather than in the more complex catecholamine field where the greater number of metabolites per amine and the considerable extent to which they are conjugated in vivo (Elchisak et al, 1983(Elchisak et al, 1977) results in relatively severe problems of specificity and sensitivity. It seems likely that voltammetry will be most applicable in the monitoring of amine metabolites rather than (as originally thought) of their parent amines, as the latter appear to occur extraneuronally at relatively low concentrations (Ungerstedt et al, 1982). It may well be that ex vivo voltammetric procedures in which small extraneuronal samples are taken either from the cerebrospinal fluid space (Sarna et al, 1983) or from brain perfusates (Ungerstedt et al, 1982) and their constituents separated by high pressure liquid chromatography before voltammetric assay will prove more widely useful than the fully in vivo method.…”

Section: Future Developmentsmentioning

confidence: 99%

“…It seems likely that voltammetry will be most applicable in the monitoring of amine metabolites rather than (as originally thought) of their parent amines, as the latter appear to occur extraneuronally at relatively low concentrations (Ungerstedt et al, 1982). It may well be that ex vivo voltammetric procedures in which small extraneuronal samples are taken either from the cerebrospinal fluid space (Sarna et al, 1983) or from brain perfusates (Ungerstedt et al, 1982) and their constituents separated by high pressure liquid chromatography before voltammetric assay will prove more widely useful than the fully in vivo method. This review has specifically described the application of voltammetry to the monitoring in vivo of transmitter amine metabolism in the brain.…”

Section: Future Developmentsmentioning

confidence: 99%

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Monitoring in vivo of transmitter metabolism by electrochemical methods

Hutson¹,

Curzon²

1983

Biochemical Journal

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IntroductionConventional methods of studying the metabolism of central neurotransmitter amines (i.e. dopamine, noradrenaline and 5-hydroxytryptamine) in rats and other small laboratory animals have two major disadvantages. Firstly, animals under investigation must be killed before determinations can be made and secondly, intra-and extra-neuronal metabolism are not differentiated. Thus, determinations can only be made at one time per animal and information is not obtained on whether transmitters or metabolites measured reflect functional activity, i.e. transmitter molecules which had been available to receptors. Also, standard methods of measuring transmitter turnover cannot be applied to individual rats but require the use of groups of animals on which changes of amine, amine precursor or metabolite concentration are measured over a period after the administration of drugs which block transmitter synthesis or catabolism or egress of amine metabolites from the central nervous system.

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Section: Future Developmentsmentioning

confidence: 99%

Section: Future Developmentsmentioning

confidence: 99%

Monitoring in vivo of transmitter metabolism by electrochemical methods

Hutson¹,

Curzon²

1983

Biochemical Journal

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“…Microdialysis has been proposed as a method for monitoring in vivo neurotransmitter release, in brain and peripheral tissue (Ungerstedt et al 1982;see Ungerstedt 1984see Ungerstedt , 1991, and it has been widely used for monitoring the effects of amphetamines (Zetterström et al 1983(Zetterström et al , 1986Westerink et al 1987;Butcher et al 1988;Hurd and Ungerstedt 1989a,b), including those of Meth (O'Dell et al 1991;Nash and Yamamoto 1992;Holson et al 1996;Sabol et al 2001).…”

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confidence: 99%

“…It is our experience, when not primarily interested in behaviour (e.g. Ungerstedt et al 1982;Zetterström et al 1986), that the presence or absence of anaesthesia is related to rather quantitative than qualitative pharmacological differences (see Herrera-Marschitz et al 1992You et al 1999;Adachi et al 2001). Thus, we have chosen a protocol controlling general physiological conditions, but concentrating on the interactions among several putative neurotransmitter systems.…”

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confidence: 99%

Effect of single and repeated methamphetamine treatment on neurotransmitter release in substantia nigra and neostriatum of the rat

Bustamante

You²,

Castel

et al. 2002

Journal of Neurochemistry

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The main purpose of this study was to characterize the initial neurotransmission cascade elicited by methamphetamine, analysing simultaneously with in vivo microdialysis monoamine, amino acid and neuropeptide release in substantia nigra and neostriatum of the rat. The main effect of a single systemic dose of methamphetamine (15 mg/kg, subcutaneously) was an increase in dopamine levels, both in substantia nigra ( 10-fold) and neostriatum ( 40-fold), accompanied by a significant, but lesser, increase in dynorphin B ( two-fold, in both regions), and a decrease in monoamine metabolites. A similar effect was also observed after local administration of methamphetamine (100 lM) via the microdialysis probes, but restricted to the treated region. In other experiments, rats were repeatedly treated with methamphetamine or saline, with the last dose administered 12 h before microdialysis. Dopamine K + -stimulated release was decreased following repeated methamphetamine administration compared with that following saline, both in the substantia nigra (by 65%) and neostriatum (by 20%). In contrast, the effect of K + -depolarization on glutamate, aspartate and GABA levels was increased following repeated administration of methamphetamine. In conclusion, apart from an impairment of monoamine neurotransmission, repeated methamphetamine produces changes in amino acid homeostasis, probably leading to NMDA-receptor overstimulation.

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“…As an alternative, the microdialysis technique might provide dialysates of the extracelhflar tissue fluid, as shown more than 20 years ago, when amino acids and neurotransmitters were analysed in brain dialysates [5,6]. In these studies the dialysates were collected in fractions, and the analytes were measured intermittently [7]. This technique has been used for the estimation of various substrates including glucose in extracellular space of different regions [8,9].…”

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confidence: 99%

On line continuous monitoring of subcutaneous tissue glucose in men by combining portable glucosensor with microdialysis

et al. 1992

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Summary.For the normalisation of blood glucose levels in diabetic patients by feedback controlled insulin delivery, a self-manageable and reliable method for continuous glucose estimation is still not available. By combining a commercially available needle type dialysis probe (molecular cutoff 20,000 Da) with a sensitive glucose sensor, we obtained a device for continuous glucose measurement in dialysate. This device was tested in healthy volunteers during a 75-g oral glucose tolerance test and in Type 2 (non-insulin-dependent) diabetic patients. Venous glucose and subcutaneous sensor signal were followed for 300 min (ten healthy subjects), 21 h (three healthy subjects) or 9 h (seven Type 2 diabetic patients). The recovery of the microdialysis was interindividually different, but after calibration, glucose levels in the dialysate and subcutaneous glucose sensor signal correlated well (r = 0.84-0.95). Under the assumption of a physiologic and technical delay between intravenous and subcutaneous glucose, correlation coefficient between intravenous glucose and subcutaneous sensor signal ranged from 0.60 to 0.93. We conclude that changes in blood glucose could be monitored in the subcutaneous tissue by the microdialysis technique in a continuous on line manner.

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Dopamine Synaptic Mechanisms Reflected in Studies Combining Behavioural Recordings and Brain Dialysis

Cited by 142 publications

References 10 publications

Monitoring in vivo of transmitter metabolism by electrochemical methods

Monitoring in vivo of transmitter metabolism by electrochemical methods

Effect of single and repeated methamphetamine treatment on neurotransmitter release in substantia nigra and neostriatum of the rat

On line continuous monitoring of subcutaneous tissue glucose in men by combining portable glucosensor with microdialysis

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