Dopaminergic Neurons Derived from Human Induced Pluripotent Stem Cells Survive and Integrate into 6-OHDA-Lesioned Rats

Cai, Jingli; Yang, Ming; Poremsky, Elizabeth; Kidd, Sarah K.; Schneider, Jay S.; Iacovitti, Lorraine

doi:10.1089/scd.2009.0319

Cited by 134 publications

(107 citation statements)

References 25 publications

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“…Moreover, genetic strategies, such as transcription factor (TF) overexpression, have been developed to further enhance the efficiency of hESC and hiPSC differentiation into DA neurons [16,17]. However, all these protocols require several intermediate steps and many different cell culture conditions [10,15,18,19]. Moreover, these protocols are extremely laborious and time-consuming, in particular when hiPSC-derived DA neurons are expected to acquire evident functional properties.…”

Section: Introductionmentioning

confidence: 99%

Rapid Generation of Functional Dopaminergic Neurons From Human Induced Pluripotent Stem Cells Through a Single-Step Procedure Using Cell Lineage Transcription Factors

Theka

Caiazzo

Dvoretskova

et al. 2013

Stem Cells Translational Medicine

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Current protocols for in vitro differentiation of human induced pluripotent stem cells (hiPSCs) to generate dopamine (DA) neurons are laborious and time-expensive. In order to accelerate the overall process, we have established a fast protocol by expressing the developmental transcription factors ASCL1, NURR1, and LMX1A. With this method, we were able to generate mature and functional dopaminergic neurons in as few as 21 days, skipping all the intermediate steps for inducting and selecting embryoid bodies and rosette-neural precursors. Strikingly, the resulting neuronal conversion process was very proficient, with an overall efficiency that was more than 93% of all the coinfected cells. hiPSC-derived DA neurons expressed all the critical molecular markers of the DA molecular machinery and exhibited sophisticated functional features including spontaneous electrical activity and dopamine release. This one-step protocol holds important implications for in vitro disease modeling and is particularly amenable for exploitation in high-throughput screening protocols. STEM CELLS TRANSLATIONAL MEDICINE 2013;2:473-479

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Section: Introductionmentioning

confidence: 99%

Rapid Generation of Functional Dopaminergic Neurons From Human Induced Pluripotent Stem Cells Through a Single-Step Procedure Using Cell Lineage Transcription Factors

Theka

Caiazzo

Dvoretskova

et al. 2013

Stem Cells Translational Medicine

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“…Those tissues include fat (Sun et al 2009 ) , blood (Ye et al 2009b ) , biopsy specimens, skin, plugged hair (Aasen et al 2008 ) , and extracted teeth (Aasen et al 2008 ;Sun et al 2009 ;Ye et al 2009b ;Yan et al 2010 ) . Thus far in humans, skin fi broblasts and bone marrow mesenchymal cells Huangfu et al 2008 ) , keratinocytes (Aasen et al 2008 ; , peripheral blood cells (Loh et al 2009 ;Ye et al 2009b ) , melanocytes (Utikal et al 2009 ) , neural stem cells (Kim et al 2009b ) , amniotic fl uid-derived cells ( Li et al 2009 ), adipose stem cells from lipoaspirate (Sun et al 2009 ) , dental stem cells (Tamaoki et al 2010 ;Yan et al 2010 ) , and mesenchymal stem cells from umbilical cord matrix and amniotic membrane (Cai et al 2010 ) have been used for reprogramming.…”

Section: Choosing Cell Sourcesmentioning

confidence: 99%

“…Examples are auditory retinal cells (Jin et al 2009 ;Comyn et al 2010 ;Lamba et al 2010 ;Parameswaran et al 2010 ) , cardiomyocytes (Narazaki et al 2008 ;Pfannkuche et al 2009 ;Tanaka et al 2009 ;Zhang et al 2009b ;CarvajalVergara et al 2010 ;Kaichi et al 2010 ;Martinez-Fernandez et al 2010 ) , insulinsecreting islet-like clusters (Tateishi et al 2008 ) , motor neurons (Dimos et al 2008 ;Ebert et al 2009 ;Hu and Zhang 2009 ;Karumbayaram et al 2009 ) , dopaminergic neurons (Wernig et al 2008 ;Cai et al 2010 ;Swistowski et al 2010 ) , auditory spinal ganglion neurons (Nishimura et al 2009 ) , smooth muscle cells (Taura et al 2009b ;Xie et al 2009 ) , vascular endothelial cells (Taura et al 2009b ) , dendritic cells and macrophages (Senju et al 2009(Senju et al , 2010 , adipocytes (Tashiro et al 2009 ;Taura et al 2009a ) , osteoblasts (Tashiro et al 2009 ) , hematopoietic cells (Hanna et al 2007 ;Eminli et al 2009 ;Kaufman 2009 ;Lu et al 2009 ;Okabe et al 2009 ;Raya et al 2009 ;Kaneko et al 2010 ) , and endothelial progenitor cells (Xu et al 2009 ;Abaci et al 2010 ;…”

Section: Differentiation Of Obtained Ips Cells To Desired Cell Typesmentioning

confidence: 99%

Mesenchymal Stem Cells: Possibilities of New Treatment Options

Tokcaer-Keskin

Koçak

Gürsel

et al. 2012

Adult and Embryonic Stem Cells

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“…Global gene expression profiles and DNA methylation patterns of iPSCs proved to be nearly indistinguishable from those of ESCs. They could maintain their self-renewal when cultured under ESC conditions and could be differentiated to cells of all three germ layers [13][14][15]. Moreover, they developed teratomas when injected into immunodeficient mice, and they could contribute to the development of chimeric mice, a major criterion for pluripotent stem cells.…”

Section: Induced Pluripotent Stem Cellsmentioning

confidence: 99%

Concise Review: Induced Pluripotent Stem Cells and Lineage Reprogramming: Prospects for Bone Regeneration

et al. 2011

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Bone tissue for transplantation therapies is in high demand in clinics. Osteodegenerative diseases, in particular, osteoporosis and osteoarthritis, represent serious public health issues affecting a respectable proportion of the elderly population. Furthermore, congenital indispositions from the spectrum of craniofacial malformations such as cleft palates and systemic disorders including osteogenesis imperfecta are further increasing the need for bone tissue. Additionally, the reconstruction of fractured bone elements after accidents and the consumption of bone parts during surgical tumor excisions represent frequent clinical situations with deficient availability of healthy bone tissue for therapeutic transplantations. Epigenetic reprogramming represents a powerful technology for the generation of healthy patientspecific cells to replace or repair diseased or damaged tissue. The recent generation of induced pluripotent stem cells (iPSCs) is probably the most promising among these approaches dominating the literature of current stem cell research. It allows the generation of pluripotent stem cells from adult human skin cells from which potentially all cell types of the human body could be obtained. Another technique to produce clinically interesting cell types is direct lineage reprogramming (LR) with the additional advantage that it can be applied directly in vivo to reconstitute a damaged organ. Here, we want to present the two technologies of iPSCs and LR, to outline the current states of research, and to discuss possible strategies for their implementation in bone regeneration.

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Dopaminergic Neurons Derived from Human Induced Pluripotent Stem Cells Survive and Integrate into 6-OHDA-Lesioned Rats

Cited by 134 publications

References 25 publications

Rapid Generation of Functional Dopaminergic Neurons From Human Induced Pluripotent Stem Cells Through a Single-Step Procedure Using Cell Lineage Transcription Factors

Rapid Generation of Functional Dopaminergic Neurons From Human Induced Pluripotent Stem Cells Through a Single-Step Procedure Using Cell Lineage Transcription Factors

Mesenchymal Stem Cells: Possibilities of New Treatment Options

Concise Review: Induced Pluripotent Stem Cells and Lineage Reprogramming: Prospects for Bone Regeneration

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