Doppel gene polymorphisms in Portuguese sheep breeds: Insights on ram fertility

Pereira, Rúben; Mesquita, Patrı́cia; Batista, Marcus Vinícius de Aragão; Baptista, M.C.; Barbas, João Pedro; Pimenta, Jorge; Santos, Ingrid; Marques, Mateus; Vasques, M.I.; Pereira, Martinha; Silva, F. Santos; Sousa, M. C. Oliveira; Fontes, Carlos M. G. A.; Horta, A.E.M.; Prates, J. A. M.; Marques, C.C.

doi:10.1016/j.anireprosci.2008.10.003

Cited by 24 publications

(32 citation statements)

References 26 publications

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“…It was recently reported that supplementation of the embryo culture system with P4 had a positive effect on the early stages of mammalian embryo development (Aparicio et al, 2011). In contrast to these results, Pereira et al (2009) suggested that P4 may affect embryo in vitro culture. In these experiments presumptive zygotes were transferred into embryo growth medium supplemented in Experiment 1 with bovine oviduct epithelial cells (BOEC) (n = 378) and BOEC plus onapristone (OP), (n = 325); in Experiment 2 with granulosa cells (GC), (n = 514); GC plus OP (n = 509); BOEC (n = 490); BOEC plus P4 (n = 500); BOEC plus P4 and OP (n = 502).…”

Section: The Role Of Egf Bsa and Progesterone As Media Supplements Dcontrasting

confidence: 53%

Selected molecular and microfluidic aspects of mammalian oocyte maturation-perspectives: a review

Kempisty¹,

Bukowska²,

Piotrowska³

et al. 2011

Vet. Med. - Czech

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ABSTRACT:Maturation of oocytes is the most important stage, which determines the subsequent successful fertilization, zygote formation, attainment of blastocyst stage, normal embryo growth and development, as well as appropriate implantation. Since in some species of mammals the efficiency of IVM maturation is still very low, many studies have focused on new combinations of media supplements. In some species of mammals, including pigs, mice, bovines, goats and dogs, EGF, BSA, and progesterone are successfully used as an enhancer of the IVC system. Application of the Lab-on-Chip system in reproductive biology opens new possibilities for the development of techniques available for the assessment of the developmental competency or potency manifested by mammalian oocytes and embryos. In most cases, the Lab-on-Chip system was used in mice. However, an increasing number of examples are available in which the system has been applied to evaluate porcine and bovine gametes and embryos. In this article, the role of EGF, BSA and progesterone is described in relation to maturation efficiency of mammalian oocytes. Moreover, the possibilities of using microfluidics (Lab-on-Chip) for the detection of oocyte developmental competency are also shown.

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Section: The Role Of Egf Bsa and Progesterone As Media Supplements Dcontrasting

confidence: 53%

Selected molecular and microfluidic aspects of mammalian oocyte maturation-perspectives: a review

Kempisty¹,

Bukowska²,

Piotrowska³

et al. 2011

Vet. Med. - Czech

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“…For analysis and interpretation, the protein coding sequences are considered; however, only nucleotide sequences are examined so as not to dismiss the role of synonymous mutations which may have a greater effect on protein expression and conformation than previously expected. [42–46] All haplotype sequences have been deposited in GenBank (accession numbers MG856905-MG856930). The relationships among haplotypes are shown in Figure 1, with haplotypes sharing identical amino acid sequences (thus separated only by synonymous differences) shown in the same color.…”

Section: Resultsmentioning

confidence: 99%

Influence of the geographic distribution of prion protein gene sequence variation on patterns of chronic wasting disease spread in white-tailed deer (Odocoileus virginianus)

Brandt

Green

Ishida

et al. 2018

Prion

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Managing and controlling the spread of diseases in wild animal populations is challenging, especially for social and mobile species. Effective management benefits from information about disease susceptibility, allowing limited resources to be focused on areas or populations with a higher risk of infection. Chronic wasting disease (CWD), a transmissible spongiform encephalopathy that affects cervids, was detected in Colorado in the late 1960s. CWD was detected in Illinois and Wisconsin in 2002 and has since spread through many counties. Specific nucleotide variations in the prion protein gene (PRNP) sequence have been associated with reduced susceptibility to CWD in white-tailed deer. Though genetic resistance is incomplete, the frequency of deer possessing these mutations in a population is an important factor in disease spread (i.e. herd immunity). In this study we sequenced 625 bp of the PRNP gene from a sampling of 2433 deer from Illinois and Wisconsin. In north-central Illinois where CWD was first detected, counties had a low frequency of protective haplotypes (frequency <0.20); whereas in northwestern Illinois counties, where CWD cases have only more recently been detected, the frequency of protective haplotypes (frequency >0.30) was much higher (p < 0.05). Protective haplotype frequencies varied significantly among infected and uninfected geographic areas. The frequency of protective PRNP haplotypes may contribute to population level susceptibility and may shape the way CWD has spread through Illinois. Analysis of PRNP haplotype distribution could be a useful tool to assess CWD risk and allocate resources to contain and reduce the spread of infection.

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“…IVC, on the contrary, is achieved in a controlled atmosphere at 38.5-39°C with 5% O 2 , 5% CO 2 , and 90% N 2 . This medium is usually composed of synthetic oviductal fluid supplemented with amino acids, bovine serum albumin (BSA), and/or serum [112,122], although the use of some media with oviduct cells or fetal fibroblasts was also reported [138]. In this stage, it is important to keep an oxygen low concentration to prevent oxidation [139] and the deleterious effects of reactive oxygen species (ROS).…”

Section: In Vitro Culture (Ivc)mentioning

confidence: 99%

“…Tissue Culture Medium (TCM199) supplemented with bicarbonate, pyruvate, LH, FSH, and estradiol (E 2 ) and with 10% fetal calf serum [10,101] or 10% estrous sheep serum [50,122] is the widest used medium for IVM. Nevertheless, other media and supplementation can be also used.…”

Section: In Vitro Maturation (Ivm)mentioning

confidence: 99%

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Cryopreservation of Sheep Produced Embryos – Current and Future Perspectives

Romão¹,

Marques²,

Bettencourt³

et al. 2016

Insights From Animal Reproduction

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Due to economical and scientific limitations, sheep embryo reproductive technologies are less commercially applied than in other animal species. However, it is very clear that, in the near future, those techniques are expected to have a central role in animal production as a consequence of genetic and reproductive demands. One drawback is that results obtained after sheep embryo cryopreservation are unattractive for commercial purposes. It is expected that a successful cryopreservation of sheep embryos can push forward all other reproductive biotechnologies in this species, such as multiple ovulation and embryo transfer (MOET), artificial insemination, or in vitro production of embryos. This paper tries to discuss the current and future perspectives of cryopreservation of in vivo-and in vitro-produced sheep embryos concerning advantages and limitations for its practical use and possible solutions for improving methods to allow a higher survival rate of cryopreserved embryos.

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Doppel gene polymorphisms in Portuguese sheep breeds: Insights on ram fertility

Cited by 24 publications

References 26 publications

Selected molecular and microfluidic aspects of mammalian oocyte maturation-perspectives: a review

Selected molecular and microfluidic aspects of mammalian oocyte maturation-perspectives: a review

Influence of the geographic distribution of prion protein gene sequence variation on patterns of chronic wasting disease spread in white-tailed deer (Odocoileus virginianus)

Cryopreservation of Sheep Produced Embryos – Current and Future Perspectives

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