Dormancy release and seed ageing in the endangered species Silene diclinis

Mira, Sara; González‐Benito, M. Elena; Ibars, Ana María; Estrelles, Elena

doi:10.1007/s10531-010-9833-x

Cited by 32 publications

(20 citation statements)

References 27 publications

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“…Intraspecific variation in seed response to GA3 also could explain that while our data show that GA3 is detrimental to seed germination, previous results indicated that GA3 allowed high germination (Mira et al, 2015b). Intraspecific variation has been interpreted as one of the most important survival strategies for species growing under variable and unpredictable environmental conditions, as in Mediterranean forests, either in germination requirements (Kigel, 1995;Baskin & Baskin, 2014), seed dormancy (Pérez-García et al, 2012;Copete et al, 2014) or seed longevity (Lazar et al, 2014;Mira et al, 2011aMira et al, , 2011bMira et al, , 2015a.…”

Section: Discussionmentioning

confidence: 55%

Seed germination of Phillyrea angustifolia L., a species of difficult propagation

2017

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Aim of study: The purpose was to determine the type of dormancy and the optimal germination conditions of Phillyrea angustifolia (Oleaceae) seeds.Area of study: Germination requirements of P. angustifolia seeds collected from wild plants growing in the province of Ávila (Central Spain) were studied.Materials and methods: Seed water uptake was measured. Seeds with and without an endocarp were germinated at different temperatures, and several treatments were tested.Main results: The lignified endocarp interferes mechanically with the emergence of the radicle, and the treatments that achieved the highest germination percentages were the total removal of the endocarp with pliers (84%) or the immersion in liquid nitrogen for 1 min (97%). Scarification with concentrated sulphuric acid did not significantly increase germination compared to the control seeds, and treatments with dry heat or wet heat were detrimental to seed germination. The optimum temperature for germination was 15 ºC. A pre-sowing treatment of soaking in distilled water for 24 h slightly increased germination speed. Neither cold stratification at 5 ºC nor soaking in a gibberellic acid solution improved seed germination.Research highlights: Phillyrea angustifolia seeds have physiological dormancy -that is, the embryo does not have enough growth potential to overcome the mechanical restriction of the lignified endocarp. The seeds do not exhibit physical dormancy, given their water-permeable lignified endocarp. Our results suggest that the optimum germination protocol for P. angustifolia would be the total removal of the endocarp or immersion in liquid nitrogen for 1 min, followed by immersion in distilled water for 24 h and then seed incubation at 15 ºC in light or darkness.

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Section: Discussionmentioning

confidence: 55%

Seed germination of Phillyrea angustifolia L., a species of difficult propagation

2017

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“…Within Brassicaceae, longevity was found to vary greatly among wild species stored both at high and low humidity (Mira et al, 2014). Differences in viability results among species or seed lots may sometimes result from some of the seeds used to compare being particularly deteriorated, as was the case with DT6 in the present study, or having developed secondary dormancy during storage (Mira et al, 2011b;van Treuren et al, 2013).…”

Section: Discussionmentioning

confidence: 65%

Germination and electrical conductivity tests on artificially aged seed lots of 2 wall-rocket species

Lazar¹,

Mira

Pamfil³

et al. 2014

Turk J Agric For

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The aim of this study was to determine whether the loss of germinability and vigour in wall-rocket seed lots was related to electrolyte leakage. Ten seed lots of 2 wall-rocket species (9 of Diplotaxis tenuifolia and one of D. erucoides (Brassicaceae)) were stored at 45 °C and 70% relative humidity and electrolyte leakage was measured using the conductivity test. Small differences in longevity were found among species or seed lots, suggesting that a similar relative longevity might be expected within the genus Diplotaxis after artificial ageing, and that it will be above the average of that found in other species of the same family at similar storage conditions. Electrolyte loss was strongly related to loss of seed viability. There was a negative linear relationship between conductivity and seed germination (R 2 = 0.85), and a positive relationship with T 50 (R 2 = 0.77). Small differences were found among species or seed lots regarding the correlation between electrolyte leakage and seed germination and vigour. Our results indicate that the conductivity method is able to predict the viability of wall-rocket seeds stored at 45 °C and 70% RH, with the same regression equation of conductivity and germination (-33.97x + 135.59) being able to predict the viability of different seed lots and species. A conductivity level ranging from 1.4 to 2.2 mS g -1 DW would indicate that the potential of a Diplotaxis seed lot to germinate has decreased to 80%. The correlation between electrolyte leakage and seed germination provides a valuable means for early detection of seed viability in wall-rocket seeds.

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“…Seed ageing causes changes in the organisation of lipid reserves and their thermal properties (Walters et al , 2005 b ), loss of membrane phospholipids (in Arabidopsis thaliana seeds, this may be due to phospholipase D activity; Devaiah et al , ), and lipid peroxidation resulting from ROS attack on the double bonds of unsaturated fatty acids (Priestly & Leopold, ; Murthy & Sun, ). Decreased fatty acid unsaturation, accumulation of lipid degradation products such as malondialdehyde, and increased electrolyte leakage due to membrane damage are all indicators of lipid peroxidation (Corbineau et al , ; Mira et al , ). The involvement of membrane integrity and lipid composition in seed persistence in the soil has not been explored and it is likely that fluctuating field conditions would greatly influence lipid metabolism, peroxidation and membrane stability.…”

Section: Seed Characteristics That Influence Persistencementioning

confidence: 99%

The ecophysiology of seed persistence: a mechanistic view of the journey to germination or demise

et al. 2014

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Seed persistence is the survival of seeds in the environment once they have reached maturity. Seed persistence allows a species, population or genotype to survive long after the death of parent plants, thus distributing genetic diversity through time. The ability to predict seed persistence accurately is critical to inform long-term weed management and flora rehabilitation programs, as well as to allow a greater understanding of plant community dynamics. Indeed, each of the 420000 seed-bearing plant species has a unique set of seed characteristics that determine its propensity to develop a persistent soil seed bank. The duration of seed persistence varies among species and populations, and depends on the physical and physiological characteristics of seeds and how they are affected by the biotic and abiotic environment. An integrated understanding of the ecophysiological mechanisms of seed persistence is essential if we are to improve our ability to predict how long seeds can survive in soils, both now and under future climatic conditions. In this review we present an holistic overview of the seed, species, climate, soil, and other site factors that contribute mechanistically to seed persistence, incorporating physiological, biochemical and ecological perspectives. We focus on current knowledge of the seed and species traits that influence seed longevity under ex situ controlled storage conditions, and explore how this inherent longevity is moderated by changeable biotic and abiotic conditions in situ, both before and after seeds are dispersed. We argue that the persistence of a given seed population in any environment depends on its resistance to exiting the seed bank via germination or death, and on its exposure to environmental conditions that are conducive to those fates. By synthesising knowledge of how the environment affects seeds to determine when and how they leave the soil seed bank into a resistance-exposure model, we provide a new framework for developing experimental and modelling approaches to predict how long seeds will persist in a range of environments.

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Dormancy release and seed ageing in the endangered species Silene diclinis

Cited by 32 publications

References 27 publications

Seed germination of Phillyrea angustifolia L., a species of difficult propagation

Seed germination of Phillyrea angustifolia L., a species of difficult propagation

Germination and electrical conductivity tests on artificially aged seed lots of 2 wall-rocket species

The ecophysiology of seed persistence: a mechanistic view of the journey to germination or demise

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