Dorso-ventral axis perturbation in goldfish embryos caused by heat- and pressure-shock treatments for chromosome set manipulation

Yamaha, Etsuro; Otani, Satoshi; Minami, Anzu; Arai, Katsutoshi

doi:10.1046/j.1444-2906.2002.00427.x

Cited by 24 publications

(15 citation statements)

References 16 publications

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“…In the goldfish and crucian carp ( Carassius auratus Linnaeus 1758), high pressure shock applied to the fertilized eggs results in the formation of the thin blastodiscs with poorly developed cytoplasm, delay of epiboly and suppression of the dorso ventral differentiation (Yamaha et al. ). Furthermore, high pressure that has been proved to damage DNA‐protein structure and affect gene expression (Fernandes et al.…”

Section: Discussionmentioning

confidence: 99%

Induction of Gynogenetic and Androgenetic Haploid and Doubled Haploid Development in the Brown Trout (Salmo trutta Linnaeus 1758)

Michalik

Dobosz

Zalewski

et al. 2015

Reprod Domestic Animals

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Gynogenetic and androgenetic brown trout (Salmo trutta Linnaeus 1758) haploids (Hs) and doubled haploids (DHs) were produced in the present research. Haploid development was induced by radiation-induced genetic inactivation of spermatozoa (gynogenesis) or eggs (androgenesis) before insemination. To provide DHs, gynogenetic and androgenetic haploid zygotes were subjected to the high pressure shock to suppress the first mitotic cleavage. Among haploids, gynogenetic embryos were showing lower mortality when compared to the androgenetic embryos; however, most of them die before the first feeding stage. Gynogenetic doubled haploids provided in the course of the brown trout eggs activation performed by homologous and heterologous sperm (rainbow trout) were developing equally showing hatching rates of 14.76 ± 2.4% and 16.14 ± 2.90% and the survival rates at the first feeding stage of 10.48 ± 3.48% and 12.78 ± 2.18%, respectively. Significantly, lower survival rate was observed among androgenetic progenies from the diploid groups with only few specimens that survived to the first feeding stage. Cytogenetic survey showed that among embryos from the diploid variants of the research, only gynogenetic individuals possessed doubled sets of chromosomes. Thus, it is reasonable to assume that radiation employed for the genetic inactivation of the brown trout eggs misaligned mechanism responsible for the cell divisions and might have delayed or even arrested the first mitotic cleavage in the androgenetic brown trout zygotes. Moreover, protocol for the radiation-induced inactivation of the paternal and maternal genome should be adjusted as some of the cytogenetically surveyed gynogenetic and androgenetic embryos exhibited fragments of the irradiated chromosomes.

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Section: Discussionmentioning

confidence: 99%

Induction of Gynogenetic and Androgenetic Haploid and Doubled Haploid Development in the Brown Trout (Salmo trutta Linnaeus 1758)

Michalik

Dobosz

Zalewski

et al. 2015

Reprod Domestic Animals

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“…However, high hydrostatic pressure shock, apart from destabilization of the microtubules can induce deformations of other cell organelles, disintegrate DNA-protein structure and change expression of the genes (Parkkiren et al 1993;Crenshaw et al 1996;Debec and Marcaillou 1997;Fernandes et al 2004). High pressure shock may disturb the dorso-ventral differentiation of the fish embryos when applied to the zygotes (Yamaha et al 2002). Moreover, we cannot exclude that the high hydrostatic pressure is damaging for the cytoplasmic mRNA and proteins indispensable for the proper embryonic development in the fish.…”

Section: Discussionmentioning

confidence: 96%

“…High pressure shock may disturb the dorso‐ventral differentiation of the fish embryos when applied to the zygotes (Yamaha et al. ). Moreover, we cannot exclude that the high hydrostatic pressure is damaging for the cytoplasmic mRNA and proteins indispensable for the proper embryonic development in the fish.…”

Section: Discussionmentioning

confidence: 99%

Use of Eggs Derived From the Interspecific Charr Hybrids to Induce Androgenetic Development of the Brook Charr (Salvelinus fontinalis Mitchill 1814)

Michalik

Dobosz

Wójcik

et al. 2013

Reprod Domestic Animals

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Although, brook charr (Salvelinus fontinalis Mitchill 1814) and Arctic charr (Salvelinus alpinus Linnaeus 1758) are able to cross and give fertile offspring, their androgenetic nucleocytoplasmic hybrids are not viable. To overcome incompatibility between the egg cytoplasm of one charr species and the sperm nucleus of another charr species, application of F1 interspecific hybrids as egg donors for the purpose of androgenesis has been proposed. Here, androgenetic development of the brook charr was successfully induced in the brook charr eggs and the eggs derived from the reciprocal brook charr × Arctic charr F1 hybrids. A working androgenesis protocol included inactivation of the maternal nuclear DNA achieved by irradiation of the eggs with 420 Gy of X-rays, insemination of such treated eggs with the haploid sperm cells and exposition of the haploid androgenetic zygotes to the high hydrostatic pressure shock (51.711 MPa for 4 min) applied 420 min after insemination. Androgenetic larvae that hatched from the brook charr and the hybrid eggs were shown to be homozygous brook charr individuals. Androgenetic individuals exhibited 84 chromosomes and 100 chromosome arms (FN), values characteristic for the brook charr diploid cells. Strategy hybridize first than induce androgenesis should be tested in order to provide androgenetic offspring in other salmonids that are able to cross and produce fertile offspring.

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“…7). Abnormal medaka embryos produced by ICSI also resemble fish embryos deficient in the body axis, which were produced by disruption of microtubules by cold, heat, or pressure shocks [31], or by the drug nocodazole [32]. In zebrafish, a microtubule-dependent localization of the diffusible morphogen, Squint, to the blastodisc is required to establish the dorso-ventral axis in early cleavage stages [33,34].…”

Section: Discussionmentioning

confidence: 98%

Artificial Fertilization by Intracytoplasmic Sperm Injection in a Teleost Fish, the Medaka (Oryzias latipes)1

Otani

Iwai

Nakahata

et al. 2009

Biology of Reproduction

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Intracytoplasmic sperm injection (ICSI) is a technique that has been successfully used for assisting reproduction in mammals. However, this method is still not reliable in nonmammalian species, including teleosts. We succeeded in producing medaka individuals by ICSI with a rate of 13.4% (28 hatched embryos out of 209 eggs fertilized by ICSI), the best value reported so far in teleosts, including zebrafish and Nile tilapia. Although the technique was based on that developed for mammalian eggs, some critical modifications were made to adjust it to the medaka egg, which has a thick and hard envelope (the chorion) and a single sperm entry site (the micropyle). Medaka ICSI was performed by injecting a demembranated spermatozoon into an egg cytoplasm through the micropyle 10-15 sec after egg activation induced by a piezo-actuated vibration, the site and timing of sperm penetration being consistent with those in normal fertilization in medaka. To increase the efficiency of ICSI in medaka, we found that the fertilization by ICSI should precisely mimic the fertilization by insemination with intact sperm, both spatially and temporally. The success rate of ICSI was highly variable in batches of eggs (ranging from 0% to 56%), suggesting that the conditions of eggs are important factors in stabilizing the production of individuals by ICSI. The success in medaka ICSI provides a basis for future research to understand the basic mechanisms in gamete biology of teleosts as well as for development of new technology that can yield valuable applications in fisheries science.

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Dorso-ventral axis perturbation in goldfish embryos caused by heat- and pressure-shock treatments for chromosome set manipulation

Cited by 24 publications

References 16 publications

Induction of Gynogenetic and Androgenetic Haploid and Doubled Haploid Development in the Brown Trout (Salmo trutta Linnaeus 1758)

Induction of Gynogenetic and Androgenetic Haploid and Doubled Haploid Development in the Brown Trout (Salmo trutta Linnaeus 1758)

Use of Eggs Derived From the Interspecific Charr Hybrids to Induce Androgenetic Development of the Brook Charr (Salvelinus fontinalis Mitchill 1814)

Artificial Fertilization by Intracytoplasmic Sperm Injection in a Teleost Fish, the Medaka (Oryzias latipes)1

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