Dose-dependent antioxidant responses and pathological changes in tenca (Tinca tinca) after acute oral exposure to Microcystis under laboratory conditions

Atencio, Loyda; Moreno, Isabel; Jos, Ángeles; Pichardo, Silvia; Moyano, Rosario; Blanco, Alfonso; Cameán, Ana M.

doi:10.1016/j.toxicon.2008.05.009

Cited by 106 publications

(67 citation statements)

References 54 publications

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“…Similarly, Atencio et al (2008) observed dilated Bowman's space, decrease of the glomerular component, tubular necrosis in tenca (Tinca tinca) orally exposed to cyanobacterial cells dosing 5, 11, 25 and 55 mg MC-LR/fish mixed with the food. In our previous field studies, kidney lesions were indicated by partial inosculation of foot processes of epithelial cell and proliferation of mesangial cells in glomeruli, and hydropic mitochondria in proximal tubules in fish exposed to natural cyanobacterial blooms in Meiliang Bay of Taihu (L. Li et al, , 2008.…”

Section: Introductionmentioning

confidence: 76%

Renal accumulation and effects of intraperitoneal injection of extracted microcystins in omnivorous crucian carp (Carassius auratus)

Xie

Lei

et al. 2013

Toxicon

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Section: Introductionmentioning

confidence: 76%

Renal accumulation and effects of intraperitoneal injection of extracted microcystins in omnivorous crucian carp (Carassius auratus)

Xie

Lei

et al. 2013

Toxicon

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“…Microcystins (MC) are cyanotoxins which occur in freshwaters worldwide and are produced by different genera of cyanobacteria such as Anabaena, Nostoc, Planktothrix and mainly Microcystis (Atencio et al, 2008). MCs are cyclic peptides with seven amino acids connected via peptide bonds in a cyclic conguration.…”

Section: Introductionmentioning

confidence: 99%

“…About its characteristics, the MC has a molecular weight ranging from 800-1000 Daltons; is soluble in water and unable to cross the lipid membrane of animals, plants and bacteria and is resistant to temperature, chemical hydrolysis and oxidation (Amado & Monserrat, 2010). MCs are known to affect many organisms, from microalgae to mammals (Atencio et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Biochemical and genetic alterations in the freshwater neotropical fish Prochilodus lineatus after acute exposure to Microcystis aeruginosa

Garcia

Martinez

2012

Neotrop. ichthyol.

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Microcystins are secondary metabolites produced by different species of cyanobacteria, such as Microcystis aeruginosa (MA). In this study, the biochemical and genetic effects of lyophilized MA were evaluated in the neotropical fish Prochilodus lineatus exposed to 1 or 2 mg L -1 lyophilized MA (treated group) or only water (control group) in static toxicity tests for 24 and 96 h. The gills and liver were used in the analysis of biotransformation enzymes and antioxidant defenses, blood and gill cells in genetic analysis and in brain and muscle it was determined the activity of acetylcholinesterase (AChE). The results showed the biotransformation pathway activation due to the increase in hepatic CYP1A and in branchial and hepatic glutathione Stransferase (GST). The antioxidant defense proved to be greatly affected by MA exposure leading to changes, both in gills and liver, in the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and in the content of tripeptide glutathione (GSH). Lipid peroxidation was not detected, but damage to DNA molecule was observed in blood cells. In conclusion, it can be state the lyophilized MA is able to promote changes in the biochemical and genetic parameters of P. lineatus.As microcistinas são metabólitos secundários produzidos por diferentes espécies de cianobactérias, como a Microcystis aeruginosa (MA). Neste estudo, os efeitos bioquímicos e genéticos de liofilizado de MA foram avaliados para juvenis da espécie de peixe Neotropical Prochilodus lineatus expostos a 1 ou 2 mg L -1 de liofilizado de MA (grupo tratado) ou apenas à água (grupo controle), em testes de toxicidade estáticos, durante 24 e 96 h. As brânquias e o fígado foram usados para as análises das enzimas de biotransformação e defesas antioxidantes, células do sangue e das brânquias para análises genéticas e no cérebro e músculo foi determinada a atividade da acetilcolinesterase (AChE). Os resultados mostraram ativação da via de biotransformação devido ao aumento da atividade da CYP1A hepática e da atividade da glutationa S-transferase (GST) hepática e branquial. As defesas antioxidantes foram muito afetadas pela exposição a MA levando a alterações, tanto no fígado como nas brânquias, na atividade da superóxido dismutase (SOD), da catalase (CAT), da glutationa peroxidase (GPx), glutationa redutase (GR) e no conteúdo do tripeptídeo glutationa (GSH). Apesar dessas alterações a peroxidação lipídica não foi detectada em nenhum dos tecidos, mas danos na molécula de DNA foram observados nas células do sangue. Em conclusão, pode-se afirmar que o liofilizado de MA é capaz de promover alterações em parâmetros bioquímicos e genéticos de P. lineatus.

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“…The variants of different MC has been formed mainly because the α-amino acids variations that are founding at positions two and four; for example, in MC-LR stands for leucine (L) and arginine (R) and a molecular weight of MC was ranged between 800 and 1000 daltons, its soluble in water and can't be able to cross through lipid membrane of animals, plants and bacteria and also its resistant to temperature, chemical hydrolysis, and oxidation [1]. MCs are known to effect on many organisms, from microorganism to mammals [2]. MCs can produce by different genera of Cyanobacteria such as Anabaena, Nostoc, Planktothrix, and Microcystis [2].…”

Section: Introductionmentioning

confidence: 99%

“…MCs are known to effect on many organisms, from microorganism to mammals [2]. MCs can produce by different genera of Cyanobacteria such as Anabaena, Nostoc, Planktothrix, and Microcystis [2]. The World Health Organization has been determined acceptable of the maximum limit of MC-LR in drinking water was 1 µg L-1 and for foods was 0.04 µg kg-1 day-1 consider as tolerance daily intake of MCs [3].…”

Section: Introductionmentioning

confidence: 99%

The Biochemical Alteration and Dna Damage in Rats (Rattus Rattus) After Chronic Intraperitoneally Injection to Purified Microcystin-Lr From Anabaena Circinalis

Khadairi

Al-amari

Almamoori

2017

Asian J Pharm Clin Res

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Objective: This study determined the effect of purified microcystin-leucine arginine (MC-LR) on biochemical and DNA damage parameters in rats.Methods: Utilization of preparative high-performance liquid chromatography in analysis, purification and collection of MC-LR, then intraperitoneally injection of purified MC-LR to rats. At the end of exposure, animals were sacrificed, and liver cell was isolated to measure the biochemical markers such as superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) as well as measured malondialdehyde (MDA), reactive oxygen species (ROS) and cytochrome P450 (Cyt P450), and DNA damage markers such as comet length, tail length, and tail moment were measured with the single cell gel electrophoresis also called comet assay. Results:The present results showed significantly increased activities of SOD as well as concentration of MDA, ROS with increasing concentration of MC-LR but the activities of CAT and GSH, as well as Cyt P450, were significantly decreased with increasing MC-LR dose while makers of DNA damage such as comet length, tail length, and tail moment also significantly increased with increasing MC-LR dose. Conclusion:This study demonstrated that chronic exposure to MC-LR toxin can induce alteration of biochemical and DNA damage markers.

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Dose-dependent antioxidant responses and pathological changes in tenca (Tinca tinca) after acute oral exposure to Microcystis under laboratory conditions

Cited by 106 publications

References 54 publications

Renal accumulation and effects of intraperitoneal injection of extracted microcystins in omnivorous crucian carp (Carassius auratus)

Renal accumulation and effects of intraperitoneal injection of extracted microcystins in omnivorous crucian carp (Carassius auratus)

Biochemical and genetic alterations in the freshwater neotropical fish Prochilodus lineatus after acute exposure to Microcystis aeruginosa

The Biochemical Alteration and Dna Damage in Rats (Rattus Rattus) After Chronic Intraperitoneally Injection to Purified Microcystin-Lr From Anabaena Circinalis

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