Dose- Dependent Effect of  Tribulus Terrestris  Dry Extract  on Reproductive Organs of Growing Male Rabbits

Abadjieva, Desislava; Григорова, Светлана; Gjorgovska, Natasha; Kistanova, Elena

doi:10.54865/mjas1991019a

Cited by 3 publications

(4 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The histopathological sections indicated in the 2nd and 3rd groups were due to the effects of TT and vitamin E which positively influenced the development of gonadal tissue (Figures 5 and Administration of TT at the dose of 2.50 mg/kg of body weight positively influenced the development of gonadal tissue. Moreover, in the aforementioned group, the population of cells in the lumen of the seminiferous tubules was higher in comparison with the control group (13). Moreover, sperm cells were noticed in the lumen of the seminiferous tubules of the rabbits receiving TT in the dose of 5.00 mg/kg of body weight according to Abadjieva, Grigorova (13).…”

Section: Discussionmentioning

confidence: 93%

“…Moreover, in the aforementioned group, the population of cells in the lumen of the seminiferous tubules was higher in comparison with the control group (13). Moreover, sperm cells were noticed in the lumen of the seminiferous tubules of the rabbits receiving TT in the dose of 5.00 mg/kg of body weight according to Abadjieva, Grigorova (13).…”

Section: Discussionmentioning

confidence: 93%

“…Abadjieva, Grigorova (13) indicated that TT can affect the body weight of rabbits and testicle weight. It should be noted that this effect is dose-dependent; therefore, the low doses (2.50 mg/kg of body weight) used in the present investigation can be relied upon as the most effective and harmless doses, as they ensure maximum body weight gain as well as the normal development of histological structures in the testes and epididymis.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Histological Study of Alteration in Testes and Epididymis of Domestic Rabbits Caused by Tribulus Terrestris and Vitamin E

2023

ARI

View full text Add to dashboard Cite

Tribulus Terrestris (TT) is a common herbal plant with different categories that grows in many countries of the world. Traditional Chinese and Indian therapies have used TT for infertility treatment and also as a powerful antioxidant agent. Therefore, this study aimed to use this plant supplemented with vitamin E to study their combined effects on the histological condition of the testicle and epididymis of rabbits. This study was performed on 28 healthy male rabbits (445-950 g, 2.0-3.0 months old) that were randomly divided into four groups (n=7). All animals were subjected to clinical examination to ensure that they were free of external and internal parasites with the use of some preventive treatments. The animals were housed individually (cage size: 50 cm×50 cm×40 cm) over the 60-day study period starting from January 2022, with an adaptation period of two weeks. Tribulus Terrestris and vitamin E treatments were as follows: the first group (G1) was daily fed on a standard diet and kept as the control group, the second group (G2) was daily fed on the same ration plus 1 g of TT (animal/daily), the third group (G3) was daily fed on the same ration plus 1 g of TT supplemented with 60 mg of vitamin E (orally) (animal /daily), and the fourth group (G4) was daily fed on the same ration, with the addition of 60 mg vitamin E per animal (orally). The morphometric investigation, macroscopic variables (including body weight, testicular weight, and volume), and the microscopic parameters of the testicular seminal tubule were measured. The histological section showed the absence of negative effects after the oral administration of TT at a dose of 1 g per day and 60 IU vitamin E for each animal. However, there was a positive effect on spermatogonia and spermatocytes in all animals, while the spermatogonia in the experimental groups were more dense, especially in the second and third groups, compared to the control group. The seminiferous tubules were significantly lined with spermatogonia, spermatocytes, and round spermatids (P<0.5) in the experimental groups, compared to the control group. Nevertheless, the epididymis tissue did not show traces of histological changes, such as epididymal hyperplasia. Sperms were more frequent in the lumens of the epididymis as well as the lumens larger than those of the control. Based on the results of this study, it can be concluded that the pole plant and vitamin E have a positive effect on the epithelial lining of the seminiferous tubules and the epididymis with an increase in sperm formation and differentiation towards maturity.

show abstract

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Histological Study of Alteration in Testes and Epididymis of Domestic Rabbits Caused by Tribulus Terrestris and Vitamin E

2023

ARI

View full text Add to dashboard Cite

show abstract

“…It was claimed that Tribulus extracts, and dietary supplements improved male and female libido disorders, impotence, infertility, and sperm mobility in addition to improving muscle tone and spermatogenesis (Kostova and Dinchev, 2005). Abadjieva et al (2019) analyzed the effect of Tribulus on the reproductive organs of growing male rabbits during 42 days. With increasing dosage there were changes in testicular weight, also the extract had a positive impact on the development of gonadal tissue.…”

Section: Progesterone and Testosterone Secretionmentioning

confidence: 99%

The Potential Effects of Tribulus Terrestris L. On Cellular Parameters and Steroidogenesis in Vitro

Zuščíková¹,

Greifová²,

Knížatová³

et al. 2022

J microb biotech food sci

View full text Add to dashboard Cite

Tribulus terrestris L. is an herbaceous plant native to India, widely used as a natural sexual stimulant by traditional medicine in China, India, and Greece, and also for the treatment of various diseases for example low blood pressure, cardiovascular diseases, kidney, and skin diseases, etc. Tribulus extracts are rich in numerous biologically active compounds, such as steroidal saponins, alkaloids, lignan amides, and flavonoids, which are useful for the effective treatment of sexual performance, hormonal and sexual problems. The main goal of our in vitro study was to demonstrate the potential consequences of Tribulus terrestris L. extract on TM3 cell parameters such as cell viability, membrane integrity, lysosomal activity, and steroid hormone secretion. The extract was applied to mice Leydig cells at various concentrations (ranging from 37.5 µg/mL to 600 µg/mL) for 24 h. Based on the obtained data, we may claim statistically significant inhibition of cell viability at 300 µg/mL (P < 0.0001) and 600 µg/mL (P < 0.0001). At the same concentrations, there was a statistically significant decline in both cell membrane integrity (P < 0.05; P < 0,0001) and lysosomal activity (P < 0.05; P < 0.001). Hormone secretion was significantly increased at 200 µg/mL (P < 0.05). To better understand how Tribulus terrestris L. affects the reproductive systems of animals or humans, further research must be done.

show abstract

Potential Protective Effects of Tribulus terrestris Extract (TTE) against Reproductive Damage Induced by Cytarabine (Ara-C) Chemotherapy in Male Rats: Histopathological Study

Zahra Mohammed Hashim,

Mayada S. Hassan,

Raeed Altaee

2024

Aca. Intl. J. V. Med.

View full text Add to dashboard Cite

The cytotoxic effects of many cancer medications cause different general side effects and defects in spermatogenesis, so still a concern that requires additional enhancement. The study was performed to assess the preventive benefits of Tribulus terrestris extract (TTE) against reproductive damage induced by cytarabine (Ara-C) chemotherapy. Twenty mature male rats will be divided into four groups. Each group contains 5 rats. A control group is included in the first group, T. terrestris extract (250 mg/kg) is given to the second group, Ara-C (25 mg/kg BW) is given to the third group, and the fourth group receives both an oral dose of TTE (250 mg/kg BW) and an intraperitoneal dosage of Ara-C (25 mg/kg BW) for a total of 28 days. After the end of the experiment tissue samples in testes and epididymis are taken for the necessary histopathological changes that revealed detectable spermatogenesis degeneration. Treatment caused spermatocytes disruption, significant histopathological alterations, rounded, shrunken seminiferous tubules with less cellular epithelium, degeneration in germinal epithelium represented by depletion with damaged Leydig cells, and this may result in reduced rat fertility. The results of the study indicate that Tribulus terrestris exhibits significant histological alterations, as evidenced by an increase in sperm growth, in contrast to Ara C group, which displayed some detrimental effects.

show abstract

Dose- Dependent Effect of Tribulus Terrestris Dry Extract on Reproductive Organs of Growing Male Rabbits

Cited by 3 publications

References 8 publications

Histological Study of Alteration in Testes and Epididymis of Domestic Rabbits Caused by Tribulus Terrestris and Vitamin E

Histological Study of Alteration in Testes and Epididymis of Domestic Rabbits Caused by Tribulus Terrestris and Vitamin E

The Potential Effects of Tribulus Terrestris L. On Cellular Parameters and Steroidogenesis in Vitro

Potential Protective Effects of Tribulus terrestris Extract (TTE) against Reproductive Damage Induced by Cytarabine (Ara-C) Chemotherapy in Male Rats: Histopathological Study

Contact Info

Product

Resources

About