Dose Response of Viola odorata on Meiotic and Mitotic Chromosomes of Vicia faba

Asthana, Monika

doi:10.9734/bjpr/2014/5998

Cited by 8 publications

(8 citation statements)

References 18 publications

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“…This reduction in mitotic activity could be attributed to inhibition of DNA synthesis or a blocking in the G2 phase of the cell cycle, preventing the cell from entering mitosis (Talukdar, 2013). The depression in MI has been also suggested as a result of physiological changes induced by the extract in nuclear chromatin and chromosomal anomalies resulting in deficiency of nucleic acid and associated proteins (Asthana and Kumar, 2014). In this study, Inhibition of MI in barely dividing cells probably is owing to the mutagenic compounds in the eucalyptus extract through their action on spindle assembly or cell cycle regulators (Dragoeva et al, 2015).…”

Section: Cytotoxicity Assaymentioning

confidence: 64%

“…The bridges in the cells were probably formed due to breakage and fusion of chromatids or subchromatids. Bridges occurrence could be attributed to chromosomal stickiness, breakage and reunion and unequal translocation or inversion of chromosome segments (Singh, 2002).Vagrants and laggards (rarely noticed in this study), were possibly formed due to the interference of mutagen with spindle apparatus proteins causing inhibition of centromeric and spindle activity, and subsequent failure of chromosomes to move toward either of the poles (Asthana and Kumar, 2014). The presence of bridges, laggards and fragments indicate the clastogenic effects of eucalyptus extract on Hordeum cells.…”

Section: Genotoxicity Assaymentioning

confidence: 84%

“…Disturbed metaphases and anaphases, the most common aberrations in this study, have been considered as indicators of microtubule malformation effect of Eucalyptus extract (Dragoeva et al, 2015). Stickiness of metaphase chromosomes (the second major chromosome aberration), usually leads to the formation of anaphase and telophase bridges causing inhibition of metaphase and cytokinesis, respectively and thus retarding cell division (Asthana and Kumar, 2014). DNA depolymerization, partial dissolution of nucleoproteins, breakage and exchanges of the basic folded units of chromatids, have been indicated as the main sources of stickiness.…”

Section: Genotoxicity Assaymentioning

confidence: 98%

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Allelopathic activity of Eucalyptus globulus leaf aqueous extract on Hordeum vulgare growth and cytogenetic behaviour

Morsi¹,

Abdelmigid²

2016

Aust J Crop Sci

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Allelopathy is a major factor that influences the establishment of native species in eucalyptus forests. In this study, different concentrations of aqueous leaf extract of Eucalyptus globulus (5%, 10%, 5%, 25%) were evaluated for allelopathic impacts on growth and cytogenetic behaviour of barley (Hordeum vulgare) plant. Results revealed a pronounced inhibitory effect of the aqueous extract on seed germination and seedling growth of barley plant. Cytogenetic analysis showed retardation of mitotic activity as well as occurrence of high percentage of chromosomal aberrations in Hordeum plants. Both recorded growth and cytogenetic effects were dose-dependent. At concentration 25%, the lower value of mitotic index (MI=5.09%) and the higher percentage of chromosome abnormalities (CA=35.25%, compared to control (9.1% and 5.25%), respectively. The observed abnormalities were disturbance, stickiness, laggards, vagrants, bridges and micronuclei. The results confirmed the presence of water-soluble allelochemicals in Eucalyptus extract which caused potential phytotoxic, cytotoxic and genotoxic effects in barley. Hence, it is recommended that barley should not be planted close to Eucalyptus trees due to adverse effects on its growth.

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Section: Cytotoxicity Assaymentioning

confidence: 64%

Section: Genotoxicity Assaymentioning

confidence: 84%

Section: Genotoxicity Assaymentioning

confidence: 98%

See 1 more Smart Citation

Allelopathic activity of Eucalyptus globulus leaf aqueous extract on Hordeum vulgare growth and cytogenetic behaviour

Morsi¹,

Abdelmigid²

2016

Aust J Crop Sci

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“…Our mothers would take small amount or spices and relevant herbs to make the recipe to soothe our pains. It is reported that the inadvisable higher dose of recipe made by plant part poses a definite risk if it contains toxic substances [1]. Spices also enhance the taste of food; deodorize living places, adding a pleasing scent around homes and other spaces.…”

Section: Introductionmentioning

confidence: 99%

Normal Mycoflora on Spicess

Asthanam¹

2017

CTBEB

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Indian mothers use spices in kitchen and as medicinal tonics. Spices have also been reported worldwide for contamination due to fungal species which might be a risk of mycotoxins. Therefore, spices should be stored carefully and the growth of the naturally found toxic fungi should be inhibited. Fungal contamination of spices is a major impediment preventing India from export of more than 10% of its production. Normal mycoflora of frequently used spices in Indian kitchen has been reviewed herewith.

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“…In a study on novel inflorescence architecture in gamma radiation-induced faba bean mutant populations, the determinant and indeterminant mutants were characterized by Nurmansyah et al (2019) and it was reported that determinant mutants had shorter plant height, earlier maturity, lower biological and seed yield than indeterminant mutants. The potency of gamma radiation on increasing genetic diversity of morphological and agronomical traits was investigated in faba bean and demonstrated the successful program of induced mutagenesis in this species (Nurmansyah et al 2020) The chromosomes of faba bean because of the easy accessibility, big size, small number (2n=12), and recognizable properties are suitable for research purposes such as mutation breeding and studying chromosome anomalies (Sharma et al 2009, Asthana andKumar 2014). Although the application of gamma rays to create a population with a large genetic diversity and access to mutants with appropriate properties has been accepted by researchers, the presence of various chromosomal anomalies in the population is inevitable.…”

mentioning

confidence: 99%

Effects of Gamma Radiation on Mitosis and Meiosis Anomalies and Agronomic Features in M2 Plants of <i>Vicia faba</i> cv. Saraziri

Siahpoosh

Nasrollahi

Sekhavat³

2020

CYTOLOGIA

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Investigation of aberrations caused by gamma radiation in mitosis and meiosis might be fundamental to elucidate the interaction between radiations and biological systems. To identify the effects of the various doses of gamma radiation (25, 35, 45, 55 Gy) on mitotic and meiotic traits and agronomical features, a field experiment and microscopy assays were conducted on M2 generation of Vicia faba cv. Saraziri. Various types of mitotic and meiotic anomalies including laggard chromosomes, precocious movement of chromosome, anaphase and telophase bridge, chromosome fragments, chromosome stickiness, micronuclei, disturbed anaphase, vagrant chromosomes, the trinucleate status in telophase and tripolar anaphase were observed in root meristem cells and pollen mother cells (PMCs). The meiosis of PMCs showed less sensitivity towards gamma radiation than mitosis of root meristem cells. Among different stages of mitosis and meiosis, the most anomalies in terms of frequency were attributed to anaphase in mitosis and telophase II in meiosis. The recorded data of different anomalies confirmed that the micronuclei in telophase of mitosis and meiosis had the most frequencies in all gamma ray doses. The effects of different doses of gamma rays on agronomic traits including the number of pods per plant, number of seeds per pod, number of seeds per plant, and seed yield per plant were significant and the values decreased as the irradiation dose increased. Moreover, increasing irradiation doses caused a delay in flowering, pod setting, and pod ripening period. The highly negative correlations between mitosis and meiosis anomalies and agronomical traits were reported in this research.

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Dose Response of Viola odorata on Meiotic and Mitotic Chromosomes of Vicia faba

Cited by 8 publications

References 18 publications

Allelopathic activity of Eucalyptus globulus leaf aqueous extract on Hordeum vulgare growth and cytogenetic behaviour

Allelopathic activity of Eucalyptus globulus leaf aqueous extract on Hordeum vulgare growth and cytogenetic behaviour

Normal Mycoflora on Spicess

Effects of Gamma Radiation on Mitosis and Meiosis Anomalies and Agronomic Features in M2 Plants of <i>Vicia faba</i> cv. Saraziri

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