DOTAREM (DOTA)–Gold-Nanoparticles: Design, Spectroscopic Evaluation to Build Hybrid Contrast Agents to Applications in Nanomedecine

Abstract

Introduction The realization of MRI contrast agents through chemical protocols of functionalization is a strong domain of research. In this work, we developed and formulated a novel hybrid gold nanoparticle system in which a gold salt (HAuCl 4 ) is combined with dotarem, an MRI contrast agent (DOTA) by chelation (Method IN) and stabilized by a lactose-modified chitosan polymer (CTL; Chitlac) to form DOTA IN-CTL AuNPs. Result and Discussion The authors demonstrate the biological efficiency of these nanoparticles in the case of three cell lines: Mia PaCa-2 (human pancreatic cancer cell line), TIB-75 (murine liver cell line) and KKU-M213 (cholangiocarcinoma cell line). DOTA IN-CTL AuNPs are stable under physiological conditions, are nontoxic, and are very efficient as PTT agents. The highlights, such as high stability and preliminary MRI in vitro and in vivo models, may be suitable for diagnosis and therapy. Conclusion We proved that DOTA IN-CTL AuNPs have several advantages: i) Biological efficacy on three cell lines: MIA PaCa-2 (human pancreatic cancer cell line), TIB-75 (murine liver cell line) and KKU-M213 (cholangiocarcinoma cell line); ii) high stability, and no-toxicity; iii) high efficiency as a PPT agent. The study conducted on MRI in vitro and in vivo models will be suitable for diagnosis and therapy.

Prospect of Gold Nanoparticles in Pancreatic Cancer

“…HAuCl 4 is combined with the MRI contrast agent dotarem and then formed into a contrast agent–AuNPs conjugate using a lactose-modified chitosan polymer. In vivo experiments demonstrate that the conjugate possesses an effective T1 high signal and features a reduced clearance time [ 53 ]. 5B1 is a fully humanized monoclonal antibody that targets the CA19-9 antigen, commonly overexpressed in PDAC [ 54 ].…”

MRI Contrast Agents in Glycobiology

“…These NPs are spherical (18 nm diameter by TEM) and stable at physiological pH, as shown by zeta potential and DLS measurements, nontoxic towards Mia PaCa-2, TIB-75, and KKU-M213 cell lines, and very efficient as photothermal therapy (PTT) agents towards those cancer cells. Preliminary in vivo MRI (3 T) studies, using male tumor-bearing BALB/cA nude mice injected at the tail vein with the NPs, showed a clear bright contrast of the tumor region 30 min after injection, on T 1w images, indicating the potential of the NPs as a theranostic agent, combining and PTT therapeutic properties in the same nanoplatform [ 53 ].…”