Double determination of long noncoding RNAs from lung cancer via multi-amplified electrochemical genosensor at sub-femtomole level

Li, Xiaoyan; Peng, Gang; Cui, Feng; Qiu, Qianying; Chen, Xiaojun; Huang, He

doi:10.1016/j.bios.2018.04.062

Cited by 38 publications

(19 citation statements)

References 37 publications

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“…In addition, non-specific binding during the magnetic separation and purification step, as well as non-specific adsorption in the detection process, could have potentially contributed to the decrease in the sensitivity. The detection limit of the assay is comparable to other existing electrochemical methods for lncRNAs detection [31,32]. be attributed to the complexity of the plasma sample, which contains millions of other biomolecules that may have the potential to interfere nonspecifically with our target.…”

Section: Detection Of Hotair Lncrna In Spiked Plasma Samplesmentioning

confidence: 89%

PCR-Free Detection of Long Non-Coding HOTAIR RNA in Ovarian Cancer Cell Lines and Plasma Samples

Soda

Umer

Kashaninejad

et al. 2020

Cancers

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Long non-coding RNA HOX transcript antisense intergenic RNA (HOTAIR) is one of the promising biomarkers that has widely been used in determining the stages of many cancers, including ovarian cancer. In cancer diagnostics, the two key analytical challenges for detecting long non-coding RNA biomarkers are i) the low concentration levels (nM to fM range) in which they are found and ii) the analytical method where broad dynamic range is required (four to six orders of magnitude) due to the large variation in expression levels for different HOTAIR RNAs. To meet these challenges, we report on a biosensing platform for the visual (colorimetric) estimation and subsequent electrochemical quantification of ovarian-cancer-specific HOTAIR using a screen-printed gold electrode (SPE-Au). Our assay utilizes a two-step strategy that involves (i) magnetic isolation and purification of target HOTAIR sequences and (ii) subsequent detection of isolated sequences using a sandwich hybridization coupled with horseradish peroxidase (HRP)-catalyzed reaction of 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide. The assay achieved a detection limit of 1.0 fM HOTAIR in spiked buffer samples with excellent reproducibility (% RSD ≤ 5%, for n = 3). It was successfully applied to detect HOTAIR in cancer cell lines and a panel of plasma samples derived from patients with ovarian cancer. The analytical performance of the method was validated with standard RT-qPCR. We believe that the proof of concept assay reported here may find potential use in routine clinical settings for the screening of cancer-related lncRNAs.

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Section: Detection Of Hotair Lncrna In Spiked Plasma Samplesmentioning

confidence: 89%

PCR-Free Detection of Long Non-Coding HOTAIR RNA in Ovarian Cancer Cell Lines and Plasma Samples

Soda

Umer

Kashaninejad

et al. 2020

Cancers

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“…A recent example of this strategy involves an RNase A-aided target recycling along with an HCR-based signal enhancement [35]. In the presence of the endoribonuclease RNase A, a small amount of RNA target triggers the formation of numerous DNA intermediates, IT, from an RNA primer probe anchored on magnetic nanospheres.…”

Section: Target Recycling Amplificationmentioning

confidence: 99%

Long noncoding RNAs: from genomic junk to rising stars in the early detection of cancer

Miranda‐Castro

de‐los‐Santos‐Álvarez

Lobo-Castañón

2019

Anal Bioanal Chem

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Despite having been underappreciated in favor of their protein-coding counterparts for a long time, long noncoding RNAs (lncRNAs) have emerged as functional molecules, which defy the central dogma of molecular biology, with clear implications in cancer. Altered expression levels of some of these large transcripts in human body fluids have been related to different cancer conditions that turns them into potential non-invasive cancer biomarkers. In this review, a brief discussion about the importance and current challenges in the determination of lncRNAs associated to cancer is provided. Different electrochemical nucleic acid-based strategies for lncRNAs detection are critically described. Future perspectives and remaining challenges for the practical implementation of these methodologies in clinical medicine are also discussed.

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“…In comparison with the large number of methods reported for the determination of miRNAs, to the best of our knowledge, only two electrochemical biosensors have been described for the determination of lncRNA .…”

Section: Electrochemical (Bio)sensing Of Nucleic Acid Epigeneticsmentioning

confidence: 99%

“…Li et al. developed a voltammetric biosensor for the dual determination of two different specific sequences (T1 and T2) from the maternally expressed gene 3 (MEG3) lncRNA. It used Fe 3 O 4 @C magnetic nanospheres functionalized with amino‐modified specific probes (P1 and P2), RNase A‐aided target recycling, DNA supersandwich‐induced signal enhancement and HCR using signalling probes modified with MB and ferrocene (Fc), and Cp‐loaded graphene‐like tungsten disulfide/dendritic gold nanostructures (WS 2 /DGN)‐modified electrodes (Figure ).…”

Section: Electrochemical (Bio)sensing Of Nucleic Acid Epigeneticsmentioning

confidence: 99%

Advances in Electrochemical (Bio)Sensing Targeting Epigenetic Modifications of Nucleic Acids

et al. 2019

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Nucleic acids epigenetic modifications including DNA and RNA methylation and post transcriptional gene regulation by noncoding RNAs (ncRNAs) such as microRNAs and long ncRNAs (lncRNAs), are of great relevance due to their role in mammalian development and the initiation and progression of various diseases like cancer. Over the past decades, a great research effort was made for determining nucleic acid methylation, electrochemical biosensors rapidly developing as efficient analytical tools for the determination of epigenetic modification of nucleic acids. In this review article, a critical overview of recently reported advances in their development and use for the determination of epigenetic modifications of nucleic acids, including the presence of methylated bases in DNA and RNA and the aberrant expression of microRNAs and lncRNAs, is provided. Special emphasis is made on methodologies successfully applied to real clinical samples, unmet challenges or key points toward future research.

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Double determination of long noncoding RNAs from lung cancer via multi-amplified electrochemical genosensor at sub-femtomole level

Cited by 38 publications

References 37 publications

PCR-Free Detection of Long Non-Coding HOTAIR RNA in Ovarian Cancer Cell Lines and Plasma Samples

PCR-Free Detection of Long Non-Coding HOTAIR RNA in Ovarian Cancer Cell Lines and Plasma Samples

Long noncoding RNAs: from genomic junk to rising stars in the early detection of cancer

Advances in Electrochemical (Bio)Sensing Targeting Epigenetic Modifications of Nucleic Acids

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