Double-enhanced lateral flow immunoassay for potato virus X based on a combination of magnetic and gold nanoparticles

Razo, Shyatesa C.; Panferov, Vasily G.; Safenkova, Irina V.; Varitsev, Yuri A.; Zherdev, Anatoly V.; Dzantiev, Boris B.

doi:10.1016/j.aca.2017.12.023

Cited by 83 publications

(39 citation statements)

References 65 publications

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“…The total cost of the assay is less than 3 €. Undergoing studies by our research group include complete validation of the nodavirus biosensor on fish culture facilities, and efforts to improve the biosensors' sensitivity of the LFB utilizing dual nanoparticles labels 26,41 and signal enhancement systems in general. Moreover, the elimination of centrifugation from the sample preparation step is among our future goals to further simplify nodavirus detection on-site.…”

Section: Discussionmentioning

confidence: 99%

Development of a Nanoparticle-based Lateral Flow Strip Biosensor for Visual Detection of Whole Nervous Necrosis Virus Particles

Toubanaki

Margaroni

Prapas³

et al. 2020

Sci Rep

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Effective analysis of pathogens causing human and veterinary diseases demands rapid, specific and sensitive detection methods which can be applied in research laboratory setups and in field for routine diagnosis. Paper lateral flow biosensors (LFBs) have been established as attractive tools for such analytical applications. In the present study a prototype LFB was designed for whole particles (virions) detection of nodavirus or fish nervous necrosis virus. Nodavirus is an important threat in the aquaculture industry, causing severe economic losses and environmental problems. The LFB was based on polyclonal antibodies conjugated on gold nanoparticles for signal visualization. Brain and retinas from fish samples were homogenized, centrifuged and the supernatant was directly applied on the LFB. formation of a red test line was indicative of nodavirus virions presence. nodavirus visual detection was completed in short time (30 min). Key factors of the LFB development influencing the assays' detection limit were characterized and the optimum parameters were determined, enabling increased efficiency, excluding non-specific interactions. Therefore, the proposed LFB assay consists a robust, simple, low cost and accurate method for detection of nodavirus virions in fish samples. The proposed biosensor is ideal for development of a commercial kit to be used on aquaculture facilities by fish farmers. It is anticipated that disease monitoring and environmental safety will benefit from the simplification of time consuming and costly procedures.Aquaculture is essential to cover fish-product demands, providing seafood in high quantities, and covering more than the half amount of fish consumed worldwide. This fact drives a strong demand for high production efficiency in aquaculture industry in order to cover the feeding needs of the world's growing population, in the middle of an increasing environmental crisis 1,2 . As a result, the aquaculture industry has continuously increased profits in a high rate. However, outbreaks of diseases caused by infectious agents are significantly restricting intensified aquaculture. According to literature 3 , 22.6% of all disease outbreaks are caused by viruses. Among these, viral nervous necrosis (VNN), also named vacuolating encephalopathy and retinopathy or encephalomyelitis, is a devastating disease, which induces cell necrosis accompanied by vacuolation in fish retina and brain. Its clinical symptoms include changes in skin color with abnormal swimming, low feed ingestion and altered buoyancy in affected fish. The disease is caused by nervous necrosis virus (NNV) or nodavirus, affecting more than 30 different fish species, worldwide. VNN causes high mortalities (80-100% in several species e.g. European sea bass), emerging as a major problem especially in the Mediterranean area, since it cannot be prevented by vaccination or effective treatment 4-6 .Fish nervous necrosis virus (belonging to Betanodavirus genus and Nodaviridae family) is icosahedral, and non-enveloped (∼25 nm in diameter). I...

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Section: Discussionmentioning

confidence: 99%

Development of a Nanoparticle-based Lateral Flow Strip Biosensor for Visual Detection of Whole Nervous Necrosis Virus Particles

Toubanaki

Margaroni

Prapas³

et al. 2020

Sci Rep

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“…99 Razo et al used two different label types, magnetic nanoparticles conjugated to biotinylated antibodies and streptavidin-coated gold nanoparticles, and a magnetic concentration step to obtain 32-fold sensitivity improvement in an LFA for potato virus X over a conventional LFA without the magnetic concentration or the gold-label-mediated aggregation. 100 Proximity-dependent. Proximity-dependent reactions effectively amplify signal by creating a signal only from the combination of detection and capture reagents.…”

Section: Reaction/signalmentioning

confidence: 99%

Sensitivity enhancement in lateral flow assays: a systems perspective

et al. 2019

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“…In Petrakova et al [19], using the examples of zearalenone and T-2 toxin, the authors showed that magnetic nanoparticles can be used as directly detectable optical markers. Razo et al [20] combined the use of magnetic immunosorbents to bind analytes, potato virus X, and functionalized gold nanoparticles, which, thanks to the biotin-streptavidin reaction, provide the formation of aggregates of two kinds of nanoparticles. This analysis was 32 times more sensitive than the nonenhanced one.…”

Section: Proper Sample For Lfiamentioning

confidence: 99%

“…The systems that implement the aggregation of several types of functionalized nanoparticles cause particular interest. Such approaches are described, for example, by Choi et al [126] with a 100-fold gain in sensitivity for the detection of troponin I using two kinds of gold nanoparticles; by Razo et al [20] with the generation of an optical signal by complexes of iron oxide nanoparticles (also used as a concentrating agent) and gold nanoparticles with a 32-fold decrease in the detection limit of potato virus X; by Taranova et al [127] with a 30-fold gain in the analysis of procalcitonin due to biotin-streptavidin aggregation of gold nanoparticles; by Shi et al [128] with complexation of gold nanoparticles of two sizes in the analysis of imidaclothiz; by Zhong et al [129] with the formation of two layers of antibody conjugates with gold nanoparticles in the detection of melamine; and by Shen et al [130] with aggregation of gold nanoparticles using polyamidoamine dendrimer, which lowered the detection limit of rabbit immunoglobulin G 20 times.…”

Section: Proper Response For Lfiamentioning

confidence: 99%

Ways to Reach Lower Detection Limits of Lateral Flow Immunoassays

Zherdev¹,

Dzantiev²

2018

Rapid Test - Advances in Design, Format and Diagnostic Applications

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This chapter considers factors influencing sensitivity of lateral flow immunoassay and modern developments that are focused on reaching lower detection limits. The existing variety of proposed approaches is classified in accordance with the "big five rules" for these assays, including proper sample, receptor, interaction, response, and output. The solutions for rapid extraction of target analytes and preventing negative influence of extractants are considered. Role to antibodies affinity and specificity is characterized. Potential of alternate bioreceptor molecules is discussed. Immunoreactants' compositions, concentrations, and locations on the test strip are characterized as factors determining assay parameters. The existing variety of labels is compared in terms of their optical and alternate registration. Tools to modulate a sequence of analytical reactions and to form aggregates of the detected labels are considered. The discussed approaches are illustrated through developments of test strips for detection of mycotoxins, veterinary drugs, and other analytes.The overall design of the immunochromatographic test strip is shown in Figure 1. It is a composite of several membranes of different structures and porosities, fixed on a support. The bundling of the test strip can vary, so it makes sense to consider its design based on what analytical tasks are being performed on its different sites.A. Typically, the lower portion of the test strip contains a sample pad. It ensures the absorption of sample components, in which the presence of the target analyte is checked.B. The following is a section with immunoreagents that are washed out during the analysis and move upward along with the components of the sample. As a rule, a conjugate pad forms this zone. It contains a conjugate of antibodies against the target analyte with a nanodispersed label-particles of colored latex, colloidal gold, and so on.The next two sections are located on the main working membrane of the test strip. C.First, there is a zone along which the movement of the absorbed components of the sample and the washed immunoreagents continues. During this movement, immune reactions occur, and specific intermolecular complexes are formed. D.Next, a mixture of reacted and unreacted molecules enters the binding area with immobilized immunoreagents. Depending on whether the target analyte was present in the sample and in what amount, binding of labeled immune complexes occurs in certain areas (in the traditional case, with the formation of narrow colored lines). Usually, additional reagents are located here to control the functionality of the test system. E. The upper part of the test strip with the final pad, usually structurally similar to the sample pad, ensures the further movement of the reaction mixture under the action of capillary forces and the washing of unreacted components from the underlying areas. These processes allow the label's binding to be evaluated correctly.Membrane components of the test strip are fixed on a plastic support and partia...

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Double-enhanced lateral flow immunoassay for potato virus X based on a combination of magnetic and gold nanoparticles

Cited by 83 publications

References 65 publications

Development of a Nanoparticle-based Lateral Flow Strip Biosensor for Visual Detection of Whole Nervous Necrosis Virus Particles

Development of a Nanoparticle-based Lateral Flow Strip Biosensor for Visual Detection of Whole Nervous Necrosis Virus Particles

Sensitivity enhancement in lateral flow assays: a systems perspective

Ways to Reach Lower Detection Limits of Lateral Flow Immunoassays

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