Double minute chromosomes and a homogeneously staining chromosome region in C3H10T1/2 murine cells transformed “in vitro” by proton radiation

Privitera, Enrica; Mosna, Giuliana; Sala, Elena; Spiga, Ivana; Gambaro, Fabio; Ghidoni, A.

doi:10.1016/0165-4608(90)90166-8

Cited by 6 publications

(3 citation statements)

References 39 publications

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“…A similar result was also reported in a subline that was isolated at an earlier passage (10). The similar substitution of the ABR/HSR for the dmins during culture in vitro was also observed in other cell lines derived from different cancers (11)(12)(13)(14)(15).…”

supporting

confidence: 81%

Evolution of Large Extrachromosomal Elements in HL-60 Cells during Culture and the Associated Phenotype Alterations

Haque

Hirano

Itoh

et al. 2001

Biochemical and Biophysical Research Communications

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supporting

confidence: 81%

Evolution of Large Extrachromosomal Elements in HL-60 Cells during Culture and the Associated Phenotype Alterations

Haque

Hirano

Itoh

et al. 2001

Biochemical and Biophysical Research Communications

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“…The presence of dmin in tumor cells had been shown to be associated with gene amplification. dmin often contain numerous copies of genes involved in cell proliferation, providing a selective advantage for in vitro and in vivo growth Privitera et al 1990). These chromosomal abberrations reflect genomic instability and a rather advanced stage of tumor progression (Bishop 1991).…”

Section: Introductionmentioning

confidence: 99%

Characterization of double minute chromosomes' DNA content in a human high grade astrocytoma cell line by using comparative genomic hybridization and fluorescence in situ hybridization

Giollant¹,

Bertrand

Verrelle

et al. 1996

Human Genetics

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The presence of double minute chromosomes (dmin) in cancer cells is known to be correlated with gene amplifications. In human high grade astrocytomas or glioblastomas, about 50% of cytogenetically characterized cases display dmin. G5 is a cell line which has been established from a human glioblastoma containing multiple dmin. In order to identify the DNA content of these dmin, three techniques were successively used: conventional cytogenetic analysis, comparative genomic hybridization (CGH). and fluorescent in situ hybridization (FISH). The karyotype of G5 cells showed numerical chromosome changes (hypertriploidy), several marker chromosomes, and multiple dmin. CGH experiments detected two strong DNA amplification areas located in 9p21-22 and 9p24, as well as an underrepresentation of chromosomes 6, 10, 11, 13, 14, and 18q. By using FISH with a chromosome 9-specific painting probe to metaphase chromosomes of the G5 cell line, dmin were shown to contain DNA sequences originating from chromosome 9. This study demonstrates the usefulness of a combination of classical karyotyping, CGH, and FISH to identify the chromosomal origin of amplified DNA sequences in dmin.

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“…In contrast, most mouse cell lines, including well-characterized “normal” cells such as NIH/3T3 and C3H10T1/2, possess aneuploid karyotypes 45, 46 . However, like their human counterparts, the mouse MRT cell line RTM639 remained primarily diploid with only a minor amount of chromosome rearrangements.…”

Section: Discussionmentioning

confidence: 99%

Establishment and characterization of MRT cell lines from genetically engineered mouse models and the influence of genetic background on their development

Kuwahara

Mora-Blanco

Banine

et al. 2012

Intl Journal of Cancer

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Malignant rhabdoid tumors (MRTs) are rare, aggressive cancers occuring in young children primarily through inactivation of the SNF5(INI1, SMARCB1) tumor suppressor gene. We and others have demonstrated that mice heterozygous for a Snf5 null allele develop MRTs with partial penetrance. We have also shown that Snf5+/− mice that lack expression of the pRb family, due to TgT121 transgene expression, develop MRTs with increased penetrance and decreased latency. Here, we report that altering the genetic background has substantial effects upon MRT development in Snf5+/− and TgT121;Snf5+/− mice, with a mixed F1 background resulting in increased latency and the appearance of brain tumors. We also report the establishment of the first mouse MRT cell lines that recapitulate many features of their human counterparts. Our studies provide further insight into the genetic influences on MRT development as well as provide valuable new cell culture and genetically engineered mouse models for the study of CNS-MRT etiology.

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Double minute chromosomes and a homogeneously staining chromosome region in C3H10T1/2 murine cells transformed “in vitro” by proton radiation

Cited by 6 publications

References 39 publications

Evolution of Large Extrachromosomal Elements in HL-60 Cells during Culture and the Associated Phenotype Alterations

Evolution of Large Extrachromosomal Elements in HL-60 Cells during Culture and the Associated Phenotype Alterations

Characterization of double minute chromosomes' DNA content in a human high grade astrocytoma cell line by using comparative genomic hybridization and fluorescence in situ hybridization

Establishment and characterization of MRT cell lines from genetically engineered mouse models and the influence of genetic background on their development

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