Double‐Side‐Coated Grid‐Type Mechanical Membrane Biosensor Based on AuNPs Self‐assembly and 3D Printing

Abstract

The membrane based on receptor functionalization provides a new paradigm for the development of mechanical biosensors. However, improvement of sensitivity and test accuracy is still a challenge for mechanical biosensors in practical application. Herein, a surface stress mechanical biosensor (MBioS) based on double‐side‐gold nanoparticale (AuNP)‐coated grid‐type polydimethylsiloxane (PDMS) membrane (D‐G‐MBioS) and 3D printing for human serum albumin (HSA) detection is developed. The surface stress is amplified by the grid coupling sandwich immune structure to improve the sensitivity of the MbioS, successfully reducing limite of detection (LOD) by two orders of magnitude. By self‐assembly of AuNPs, the double‐side‐coated PDMS membrane is prepared, and the detection accuracy of the sensor is improved. The 3D printing is employed to improve the consistency of the MBioS. The D‐G‐MBioS system is constructed, realizing the HSA detection in range of 0–50 µg mL−1 with a LOD of 20.6 ng mL−1. In addition, the D‐G‐MBioS based on sandwich assay is exhibited the high specificity, selectivity, stability, and repeatability. Especially, the D‐G‐MBioS can detect the HSA in real samples. This study provides a way to improve the sensitivity of mechanical biosensor.

Fabrication of a Free‐Standing MWCNT Electrode by Electric Field Force for an Ultra‐Sensitive MicroRNA‐21 Nano‐Genosensor

“…Au nanoparticles(AuNPs) were electrodeposited on the MWCNTs‐COOH of the electrode by chronoamperometry (CA) technique at the constant potential of −0.8 V for 500 s. The dielectric solution included 1.0 × 10 −3 m HAuCl4, 0.5 m H 2 SO 4 and 0.1 × 10 −3 m l ‐cysteine. [ 53,60 ]…”

“…Au nanoparticles(AuNPs) were electrodeposited on the MWCNTs-COOH of the electrode by chronoamperometry (CA) technique at the constant potential of −0.8 V for 500 s. The dielectric solution included 1.0 × 10 −3 m HAuCl4, 0.5 m H 2 SO 4 and 0.1 × 10 −3 m l-cysteine. [53,60] To fabricate the genosensor, 8 µL of the capture probe was coated onto the modified electrode surface. The capture probe was attached to the electrode surface by the formation of AuS bond between the thiol functional group at the 5-end of the capture probe with AuNPs, through the self-assembly process at room temperature overnight.…”

Prospects of Biosensors Based on Functionalized and Nanostructured Solitary Materials: Detection of Viral Infections and Other Risks

“…The rib waveguide cantilever was connected with hidden waveguides on silicon . For human serum albumin detection, a surface stress mechanical biosensor based on a double-sided Au NP-coated grid-type polydimethylsiloxane membrane and 3D printing has been developed …”

Multilayer Heterogeneous Membrane Biosensor Based on Multiphysical Field Coupling for Human Serum Albumin Detection

“…The potency of human serum albumin (HSA) present in normal human urine is 20 mg/mL –1 . If the HSA in urine exceeds the upper limit of this value, the risk of kidney disease is indicated . Hence, efficient detection of urinary HSA concentration can effectively diagnose and prevent kidney disease at an early stage, − and it is necessary to design a biosensor that can effectively detect the concentration of HSA.…”