Double spiral detection channel for on-chip chemiluminescence detection

Lok, Khoi Seng; Kwok, Yien Chian; Nguyen, Nam‐Trung

doi:10.1016/j.snb.2012.04.047

Cited by 15 publications

(7 citation statements)

References 32 publications

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“…Recently, many CL probes have been developed and applied to the monitoring of various compounds . Among them, luminol, regarded as a typical strong CL reagent, has been found that its light emission can be enhanced significantly when hydroxyl compounds were added into this CL reaction. − In addition, persulfate is an oxidant, which can produce weak CL emission when it is mixed with alkaline luminol. , These interesting results encourage us to explore a luminol probe-triggered CL measurement system for monitoring hydroxylated intermediates generated in AOPs.…”

Section: Introductionmentioning

confidence: 98%

Chemiluminescence as a New Indicator for Monitoring Hydroxylated Intermediates in Persulfate-Based Advanced Oxidation Processes

Chen

Teng

et al. 2019

J. Phys. Chem. C

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The formation of hydroxylated intermediates is the first step in the persulfate-based advanced oxidation processes (AOPs) and depends directly on the oxidation degree of recalcitrant pollutants. Therefore, the monitoring of hydroxylated intermediates plays a vital role in persulfate-based AOPs for evaluating the oxidation degree and understanding the degradation mechanism. In this work, based on the attractive amplification of luminol chemiluminescence (CL) signals by hydroxylated intermediates, we developed a rapid, sensitive, and simple CL-based method to monitor hydroxylated intermediate (i.e., 3,5-diethyl-1,4-benzenediol) generated in persulfate-based AOPs of 1,2-divinylbenzene (DVB). The possible mechanism of the proposed CL system was that hydroxylated intermediates could be oxidized by persulfate in alkaline solution to form superoxide anion radicals and thus lead to an increase in the luminol CL. Finally, the proposed CL system was successfully applied in monitoring hydroxylated intermediates generated during persulfate-based AOPs under different degradation conditions, whose validity and reliability were verified by liquid chromatography–mass spectrometry (LC-MS) and Fourier-transform infrared spectroscopy (FT-IR). The generality of the proposed CL system was verified by monitoring hydroxylated intermediates generated in persulfate-based AOPs of methylbenzene and benzoic acid. These results suggested that this present CL system provided a promising indicator for monitoring hydroxylated intermediates in persulfate-based AOPs.

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Section: Introductionmentioning

confidence: 98%

Chemiluminescence as a New Indicator for Monitoring Hydroxylated Intermediates in Persulfate-Based Advanced Oxidation Processes

Chen

Teng

et al. 2019

J. Phys. Chem. C

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“…Based on immunofluorescence technology, they employed the antigen–antibody reaction of lung cancer cells to realize the detection and diagnosis of early lung cancer. Lok et al [ 22 ] designed a micromixer with herringbone structures to perform luminol–hydrogen peroxide chemiluminescence detection, and the results showed that the luminescence intensity has a linear relationship with Co 2+ ion concentration or hydrogen peroxide concentration.…”

Section: Introductionmentioning

confidence: 99%

An Investigation of Flow Patterns and Mixing Characteristics in a Cross-Shaped Micromixer within the Laminar Regime

et al. 2021

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A fast mixing is critical for subsequent practical development of microfluidic devices, which are often used for assays in the detection of reagents and samples. The present work sets up computational fluid dynamics simulations to explore the flow characteristic and mixing mechanism of fluids in cross-shaped mixers within the laminar regime. First, the effects of increasing an operating parameter on local mixing quality along the microchannels are investigated. It is found that sufficient diffusion cannot occur even though the concentration gradient is large at a high Reynolds number. Meanwhile, a method for calculating local mixing efficiency is also characterized. The mixing efficiency varies exponentially with the flow distance. Second, in order to optimize the cross-shaped mixer, the effects of design parameters, namely aspect ratio, mixing angle and blockage, on mixing quality are captured and the visualization of velocity and concentration distribution are demonstrated. The results show that the aspect ratio and the blockage play an important role in accelerating the mixing process. They can improve the mixing efficiency by increasing the mass transfer area and enhancing the chaotic advection, respectively. In contrast, the inflow angle that affects dispersion length is not an effective parameter. Besides, the surface roughness, which makes the disturbance of fluid flow by roughness more obvious, is considered. Three types of rough elements bring benefits for enhancing mixing quality due to the convection induced by the lateral velocity.

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“…Most chemiluminescent biosensors rely on conventional laboratory instrumentation for the transduction of the optical signal into a digital signal. Examples include inverted optical microscope or CCD cameras [ 16 ], waveguides and in-line detection elements [ 19 ]. However, the use of transducers undermines the advantages of portability and simplicity of the microfluidic device.…”

Section: Introductionmentioning

confidence: 99%

An All-Glass Microfluidic Network with Integrated Amorphous Silicon Photosensors for on-Chip Monitoring of Enzymatic Biochemical Assay

et al. 2017

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A lab-on-chip system, integrating an all-glass microfluidics and on-chip optical detection, was developed and tested. The microfluidic network is etched in a glass substrate, which is then sealed with a glass cover by direct bonding. Thin film amorphous silicon photosensors have been fabricated on the sealed microfluidic substrate preventing the contamination of the micro-channels. The microfluidic network is then made accessible by opening inlets and outlets just prior to the use, ensuring the sterility of the device. The entire fabrication process relies on conventional photolithographic microfabrication techniques and is suitable for low-cost mass production of the device. The lab-on-chip system has been tested by implementing a chemiluminescent biochemical reaction. The inner channel walls of the microfluidic network are chemically functionalized with a layer of polymer brushes and horseradish peroxidase is immobilized into the coated channel. The results demonstrate the successful on-chip detection of hydrogen peroxide down to 18 μM by using luminol and 4-iodophenol as enhancer agent.

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Double spiral detection channel for on-chip chemiluminescence detection

Cited by 15 publications

References 32 publications

Chemiluminescence as a New Indicator for Monitoring Hydroxylated Intermediates in Persulfate-Based Advanced Oxidation Processes

Chemiluminescence as a New Indicator for Monitoring Hydroxylated Intermediates in Persulfate-Based Advanced Oxidation Processes

An Investigation of Flow Patterns and Mixing Characteristics in a Cross-Shaped Micromixer within the Laminar Regime

An All-Glass Microfluidic Network with Integrated Amorphous Silicon Photosensors for on-Chip Monitoring of Enzymatic Biochemical Assay

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