Doubled Haploids in Genetics and Plant Breeding

Forster, B. P.; Thomas, William

doi:10.1002/9780470650301.ch3

Cited by 108 publications

(117 citation statements)

References 109 publications

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“…I nbred (true-breeding) lines that are homozygous at virtually all loci enable both consistent production of superior hybrid plants as well as facile genetic analysis [1][2][3][4] . Traditional inbreeding, however, requires six to eight generations of selfing or sib-mating to generate inbred lines.…”

mentioning

confidence: 99%

A haploid genetics toolbox for Arabidopsis thaliana

et al. 2014

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Genetic analysis in haploids provides unconventional yet powerful advantages not available in diploid organisms. In Arabidopsis thaliana, haploids can be generated through seeds by crossing a wild-type strain to a transgenic strain with altered centromeres. Here we report the development of an improved haploid inducer (HI) strain, SeedGFP-HI, that aids selection of haploid seeds prior to germination. We also show that haploids can be used as a tool to accelerate a variety of genetic analyses, specifically pyramiding multiple mutant combinations, forward mutagenesis screens, scaling down a tetraploid to lower ploidy levels and swapping of nuclear and cytoplasmic genomes. Furthermore, the A. thaliana HI can be used to produce haploids from a related species A. suecica and generate homozygous mutant plants from strong maternal gametophyte lethal alleles, which is not possible via conventional diploid genetics. Taken together, our results demonstrate the utility and power of haploid genetics in A. thaliana.

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confidence: 99%

A haploid genetics toolbox for Arabidopsis thaliana

et al. 2014

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“…As a result, additive, additive-related epistasis, and linkage effects can be investigated properly. As a permanent population, DH lines can be replicated as many times as desired across different environments, seasons and laboratories, providing endless genetic material for phenotyping and genotyping and to evaluate the genotype-byenvironment interaction (Forster & Thomas, 2004;Bordes et al, 2006). In DH populations, the additive component of genetic variance is larger than that of F 2 and BC populations.…”

Section: Mapping Populationsmentioning

confidence: 99%

Progression of DNA Marker and the Next Generation of Crop Development

Utomo¹,

Wenefrida²,

Linscombe³

2012

Crop Plant

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“…In early studies, the objective of producing a haploid plant was to develop a doubled haploid (DH); a homozygous, true-breeding line to be used in a plant breeding program, and numerous reviews have been written on this topic (Thomas et al 2003;Forster and Thomas 2005;Szarejko and Forster 2007). DH lines are valued for use in breeding programs because traits can be fixed without multiple generations of back-crossing.…”

Section: Introductionmentioning

confidence: 99%

Haploids and doubled haploids in Brassica spp. for genetic and genomic research

Ferrie

Möllers

2010

Plant Cell Tiss Organ Cult

103

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The availability of a highly efficient and reliable microspore culture protocol for many Brassica species makes this system useful for studying basic and applied research questions. Microspores and microspore-derived embryos are ideal targets for modification by mutagenesis and transformation. Regenerated doubled haploid plants are widely used in breeding programs and in genetic studies. Furthermore, the Brassica microspore culture system allows the identification of genomic regions and genes involved in the microspore embryogenic response, spontaneous diploidization and direct embryo to plant conversion. This review summarizes current achievements and discusses future perspectives.

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Doubled Haploids in Genetics and Plant Breeding

Cited by 108 publications

References 109 publications

A haploid genetics toolbox for Arabidopsis thaliana

A haploid genetics toolbox for Arabidopsis thaliana

Progression of DNA Marker and the Next Generation of Crop Development

Haploids and doubled haploids in Brassica spp. for genetic and genomic research

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