Downregulated miR-486-5p acts as a tumor suppressor in esophageal squamous cell carcinoma

Abstract

Abstract. microRNAs (miRNAs/miRs) are crucial regulators of gene expression at the post-translational level through promoting mRNA degradation or the repression of translation of target genes. miRs have been confirmed to serve a dominant role in tumor biology. miR-486-5p has been ascertained to be involved in non-small-cell lung cancer, breast cancer and hepatocellular carcinoma; however, the expression and function of miR-486-5p in esophageal squamous cell carcinoma (ESCC) has yet to be elucidated. The present study aimed to analyze the expression levels of miR-486-5p in ESCC tissues and paired normal adjacent tissues, and determine the effects of miR-486-5p on esophageal cancer cells using MTT, wound scratch and apoptosis assays. The current results showed that miR-486-5p was significantly downregulated in ESCC specimens. Ectopic expression of miR-486-5p by synthetic mimics reduced cell proliferation and migration and induced increased cell apoptosis. The results indicated miR-486-5p may function as a tumor suppressor in ESCC. The present study demonstrated that miR-486-5p was downregulated in ESCC and served a anti-oncogene role in ESCC via affecting cellular migration.

DNA-methylation-mediated silencing of miR-486-5p promotes colorectal cancer proliferation and migration through activation of PLAGL2/IGF2/β-catenin signal pathways

“…Emerging evidence has revealed that miR-486-5p acts as an oncogene or tumor suppressor in various cancers, including non-small cell lung cancer, prostate cancer, esophageal squamous cell carcinoma, and papillary thyroid carcinoma 12 , 28 – 30 . However, its biological role in CRC remains elusive.…”

“…Emerging studies reported that miRNAs regulate approximate 60% protein-coding genes that are linked with several biological processes, including cell proliferation, apoptosis, invasion, chemosensitivity, and so on 9 – 11 . Recently, increasing evidence demonstrated that miR-486-5p acts as a tumor inducer or suppressor in multiple human malignancies 12 – 14 . Besides, The Cancer Genome Atlas (TCGA) database illustrated that miR-486-5p is one of the most downregulated miRNAs in CRC.…”

Over-regulation of microRNA-133b inhibits cell proliferation of cisplatin-induced non-small cell lung cancer cells through PI3K/Akt and JAK2/STAT3 signaling pathway by targeting EGFR

“…Later, the researchers continued to find the corresponding miRNA from fruit flies, human beings, plants and other eukaryotes (9). Growing evidence has suggested that they are mediators of the genetic expression, modification, transcription as well as translation (9).…”

“…Later, the researchers continued to find the corresponding miRNA from fruit flies, human beings, plants and other eukaryotes (9). Growing evidence has suggested that they are mediators of the genetic expression, modification, transcription as well as translation (9). It was found that the miRNA polymorphism, abnormal miRNA expression, as well as receptors affecting gene expression on drug uptake, metabolism and distribution pathways, as well as targeting clinical function may significantly affect the therapeutic effects of antitumor drugs.…”

“…Abnormal miRNA expression may lead to the loss or enhancement of miRNA function, thus affecting the expression levels of the target protein (9). Receptors affecting gene expression on drug uptake, metabolism and distribution pathways, as well as targeting clinical function may significantly affect the therapeutic effects of antitumor drugs (10).…”