Downregulating p22phox ameliorates inflammatory response in Angiotensin II-induced oxidative stress by regulating MAPK and NF-κB pathways in ARPE-19 cells

Qiu, Yiguo; Tao, Lifei; Lei, Chunyan; Wang, Jiaming; Yang, Peizeng; Li, Qiuhong; Lei, Bo

doi:10.1038/srep14362

Cited by 38 publications

(27 citation statements)

References 59 publications

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“…In the present study, the alterations of rat liver p22 phox levels were found to be compatible with the NF-κB levels change. As a corresponding mechanistic proposition for our results, the downregulation of p22 phox was reported to be closely connected with the suppression of the mitogen-activated protein kinase (MAPK) and NF-κB pathways (Qiu et al, 2015).…”

Section: F I G U R Esupporting

confidence: 67%

Effects of walnut oil on metabolic profile and transcription factors in rats fed high‐carbohydrate‐/‐fat diets

et al. 2020

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The aim of this study was to examine the effects of walnut oil (WO) on metabolic profile and transcription factors in rats fed high carbohydrate (HCD) and high‐fat diet (HFD). Forty‐two male rats were divided in to six groups: (a) Control, (b) WO (20 mg/kg BW), (c) HCD (20% of sucrose), (d) HCD + WO (e) HFD (42% of calories as fat), and (f) HFD + WO. HFD and HCD intake increased final body weights by 19% and 23% and visceral fat weights by 3‐ and 5‐fold, respectively (p < .05 for all). In addition, serum glucose, total cholesterol, triglyceride, and free fatty acids (FFA) insulin, leptin, and MDA levels increased in rats fed with HFD and HCD. WO supplementation improved these metabolic parameters (p < .05 for all). HFD + WO and HCD + WO treated groups had a significant reduction in serum and liver malondialdehyde (MDA) levels by 12% or 15% (p < .05 for both). In addition, WO supplementation lowered the levels of hepatic nuclear factor kappa B (NF‐κB) and NADPH oxidase subunit p22phox, whereas increased the endothelial‐NO synthase (e‐NOS), nuclear factor erythroid 2‐related factor‐2, and sirtuin‐1 levels. In conclusion, WO supplementation could alleviate the adverse impacts of both HCD and HFD in the rats. Practical applications This study suggests that WO intake can modulate carbohydrate metabolism and increase antioxidant capacity. These properties might be partially mediated through the regulation of the transcription factors and some proteins involved in energy metabolism, as well as a balance of oxidative stress, and insulin sensitivity.

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Section: F I G U R Esupporting

confidence: 67%

Effects of walnut oil on metabolic profile and transcription factors in rats fed high‐carbohydrate‐/‐fat diets

et al. 2020

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“…For instance, studies involving down-regulation of the gene encoding the p22phox enzyme, which produces reactive oxygen species, shows antiinflammation effect in ARPE-19 cells by regulating MAPK and NF-κB pathways. 104 These studies support the notions that p22phox plays an important role in inflammation accompanied by oxidative stress and implies that p22phox may provide a novel therapeutic target for oxidative induced…”

Section: Concluding Remarks and Future Considerationsmentioning

confidence: 57%

Antiangiogenic Eye Gene Therapy

Corydon

2015

Human Gene Therapy

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The idea of treating disease in humans with genetic material was conceived over two decades ago and with that a promising journey involving development and efficacy studies in cells and animals of a large number of novel therapeutic reagents unfolded. In the footsteps of this process, successful gene therapy treatment of genetic conditions in humans has shown clear signs of efficacy. Notably, significant advancements using gene supplementation and silencing strategies have been made in the field of ocular gene therapy, thereby pinpointing ocular gene therapy as one of the compelling "actors" bringing gene therapy to the clinic. Most of all, this success has been facilitated because of (1) the fact that the eye is an effortlessly accessible, exceedingly compartmentalized, and immune-privileged organ offering a unique advantage as a gene therapy target, and (2) significant progress toward efficient, sustained transduction of cells within the retina having been achieved using nonintegrating vectors based on recombinant adeno-associated virus and nonintegrating lentivirus vectors. The results from in vivo experiments and trials suggest that treatment of inherited retinal dystrophies, ocular angiogenesis, and inflammation with gene therapy can be both safe and effective. Here, the progress of ocular gene therapy is examined with special emphasis on the potential use of RNAi- and protein-based antiangiogenic gene therapy to treat exudative age-related macular degeneration.

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“…The increased NADPH oxidase expression mediates blood pressure increase and contributes to ROS production, endothelial dysfunction, inflammation and increased extracellular matrix (ECM) deposition in vascular tissues (Bruder‐Nascimento et al., ; Dikalov et al., ; Kim et al., ; Murdoch et al., ). It has been reported that inhibition of NADPH oxidase expression attenuated blood pressure increase, endothelial dysfunction and vascular remodelling in AngII‐induced hypertensive rats (Bruder‐Nascimento et al., ; Dikalov et al., ; Murdoch et al., ; Qiu et al., ). Of note, accumulating data have shown that AngII acutely increased NADPH oxidase activity without change in NADPH oxidase expression in vascular endothelial cells and vascular smooth muscle cells (Seshiah et al., ).…”

Section: Introductionmentioning

confidence: 99%

“…that inhibition of NADPH oxidase expression attenuated blood pressure increase, endothelial dysfunction and vascular remodelling in AngII-induced hypertensive rats (Bruder-Nascimento et al, 2014;Dikalov et al, 2014;Murdoch et al, 2011;Qiu et al, 2015). Of note, accumulating data have shown that AngII acutely increased NADPH oxidase activity without change in NADPH oxidase expression in vascular endothelial cells and vascular smooth muscle cells (Seshiah et al, 2002).…”

mentioning

confidence: 98%

Membrane raft redox signalling contributes to endothelial dysfunction and vascular remodelling of thoracic aorta in angiotensin II‐infused rats

Wei

et al. 2019

Experimental Physiology

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New Findings What is the central question of this study?Is the membrane raft redox signalling pathway involved in blood pressure increase, endothelial dysfunction and vascular remodelling in an angiotensin II‐induced hypertensive animal model? What is the main finding and its importance?The membrane raft redox signalling pathway was involved in endothelial dysfunction and medial remodelling in angiotensin II‐induced hypertension. Abstract The membrane raft (MR) redox pathway is characterized by NADPH oxidase activation via the clustering of its subunits through lysosome fusion and the activation of acid sphingomyelinase (ASMase). Our previous study shows that the MR redox signalling pathway is associated with angiontensin II (AngII)‐induced production of reactive oxygen species (ROS) and endothelial dysfunction in rat mesenteric arteries. In the present study, we hypothesized that this signalling pathway is involved in blood pressure increase, endothelial dysfunction and vascular remodelling in an AngII‐induced hypertensive animal model. Sixteen‐week‐old male Sprague–Dawley rats were subjected to AngII infusion for 2 weeks with or without treatment with the lysosome fusion inhibitor bafilomycin A1 and ASMase inhibitor amitriptyline. After treatments, aortas were harvested for further study. The results showed that the MR redox signalling pathway was activated as indicated by the increase of MR formation, ASMase activity and ROS production in aorta from AngII‐infused rats compared with that from control rats. MR formation and ROS production were significantly inhibited in thoracic aorta from AngII‐induced rats treated with bafilomycin A1 and amitriptyline. Both treatments significantly attenuated blood pressure increase, endothelial dysfunction and vascular remodelling including medial hypertrophy, and increased collagen and fibronectin deposition in thoracic aortas from AngII‐infused rats. Finally, both treatments significantly prevented the increase of inflammatory factors including monocyte chemotactic protein 1, intercellular adhesion molecule 1 and tumour necrosis factor α in thoracic aorta from AngII‐infused rats. In conclusion, the present study demonstrates that the MR redox signalling pathway was involved in endothelial dysfunction and medial remodelling in AngII‐induced hypertension.

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Downregulating p22phox ameliorates inflammatory response in Angiotensin II-induced oxidative stress by regulating MAPK and NF-κB pathways in ARPE-19 cells

Cited by 38 publications

References 59 publications

Effects of walnut oil on metabolic profile and transcription factors in rats fed high‐carbohydrate‐/‐fat diets

Effects of walnut oil on metabolic profile and transcription factors in rats fed high‐carbohydrate‐/‐fat diets

Antiangiogenic Eye Gene Therapy

Membrane raft redox signalling contributes to endothelial dysfunction and vascular remodelling of thoracic aorta in angiotensin II‐infused rats

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