Downregulation of long noncoding RNA LINC00460 expression suppresses tumor growth in vitro and in vivo in gastric cancer

Zhang, Shuhua; Xu, Jianqun; Wang, Hongjuan; Guo, Hongrong

doi:10.3233/cbm-182177

Cited by 23 publications

(28 citation statements)

References 16 publications

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“…LncRNAs are reported to be regulators of gene expression through a variety of mechanisms, including genomic interactions, protein amounts, miRNA competition, and chromatin modifications 15,16. Various lncRNAs are abnormally expressed in gastric cancer and regulate multiple pathological processes, including cell proliferation, cell cycle, apoptosis, metastasis, and angiogenesis 17–19. Hence, lncRNAs might be potential molecular targets for the diagnosis and treatment of gastric cancer.…”

Section: Introductionmentioning

confidence: 99%

<p>Long noncoding RNA SNHG16 silencing inhibits the aggressiveness of gastric cancer via upregulation of microRNA-628-3p and consequent decrease of NRP1</p>

Pang¹,

Zhai²,

Wang³

et al. 2019

CMAR

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Background: MicroRNA-628-3p (miR-628) has been reported to play important roles in the progression of multiple human cancer types. Nonetheless, whether the expression profile of miR-628 is altered in gastric cancer remains unclear and whether its aberrant expression plays a crucial part in the aggressiveness of gastric cancer is yet to be determined. Therefore, in this study, we systematically investigated the involvement of miR-628 in gastric cancer progression. Materials and methods: MiR-628 expression in gastric cancer tissues and cell lines were determined via reverse transcription-quantitative polymerase chain reaction (RT-qPCR). A CCK-8 assay, flow-cytometric analysis, Transwell assays, and a xenograft model experiment were performed to evaluate the influence of miR-628 overexpression on gastric cancer cells. Notably, the mechanisms underlying the tumor-suppressive activity of miR-628 in gastric cancer cells were explored by bioinformatics analysis, a luciferase reporter assay, RT-qPCR, and Western blotting. Results: MiR-628 expression was low in gastric cancer tissue samples and cell lines. The low expression of miR-628 was closely associated with the lymph node metastasis, invasive depth and TNM stage among patients with gastric cancer. Further clinical analysis indicated that patients with gastric cancer underexpressing miR-628 had a worse prognosis than did the patients with high miR-628 expression in the tumor. Overexpressed miR-628 restrained proliferation, migration, and invasion; induced apoptosis; and impaired tumor growth of gastric cancer cells. In addition, neuropilin 1 (NRP1) mRNA was validated as the direct target of miR-628 in gastric cancer. Long noncoding RNA small nucleolar RNA host gene 16 (SNHG16) was demonstrated to sponge miR-628 in gastric cancer. Moreover, miR-628 knockdown abrogated the influence of SNHG16 silencing on gastric cancer cells. Conclusion: Our findings elucidate how the SNHG16-miR-628-NRP1 pathway serves as a regulatory network playing crucial roles in gastric cancer progression, suggesting that this pathway may be a novel target of anticancer therapy.

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Section: Introductionmentioning

confidence: 99%

<p>Long noncoding RNA SNHG16 silencing inhibits the aggressiveness of gastric cancer via upregulation of microRNA-628-3p and consequent decrease of NRP1</p>

Pang¹,

Zhai²,

Wang³

et al. 2019

CMAR

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“…The effects of lncRNA LINC00460 in vivo have also been explored in kinds of cancers before. For example, Zhang S et al reported that down-regulation of lncRNA LINC00460 expression suppressed tumor growth in vivo in gastric cancer [27]. Overexpressed AGR2 was also demonstrated to contribute to growth and angiogenesis of glioblastoma in vitro and in tumor xenografts [28].…”

Section: Discussionmentioning

confidence: 99%

Long noncoding RNA LINC00460 conduces to tumor growth and metastasis of hepatocellular carcinoma through miR-342-3p-dependent AGR2 up-regulation

Han

Sui

Tao

et al. 2020

Aging

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Hepatocellular carcinoma (HCC) is the fifth most common malignant tumor in the world. It ranks third among cancer-induced deaths worldwide and has the characteristics of high metastasis and high recurrence rate. Long non-coding RNA (LncRNA) LINC00460 is significantly up-regulated in multiple types of cancers and is closely related to the progression of tumors. However, effects of LINC00460 and corresponding regulatory path in HCC are still poorly investigated. In our study, we found that expression of LINC00460 was up-regulated in HCC tissues and cell lines compared with the control. Then we revealed that knockdown of LINC00460 suppressed cell proliferation and cell mobility and induced cell apoptosis in HCC cells. Further study demonstrated that knockdown of LINC00460 suppressed the progression of HCC by elevating the expression of microRNA (miRNA, miR)-342-3p. Besides that, metastasis marker, Anterior gradient homolog 2 (AGR2) was found to be a target of miR-342-3p and overexpression of AGR2 promoted the progression of HCC. Finally, the in vivo experiments further verified the anti-tumor effects of LINC00460 / miR-342-3p / AGR2 axis in HCC. The LINC00460 / miR-342-3p / AGR2 axis exerts anti-tumor effect in HCC in vitro and in vivo, consolidating and expanding the research about targeted gene therapy for early diagnosis and treatment of HCC.

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“…These eligible studies (one nasopharyngeal carcinoma, one oesophageal squamous cell carcinoma, two colorectal cancers, 1lung adenocarcinoma, one epithelial ovarian cancer, one non-small cell lung cancer, one gastric cancer and one bladder urothelial carcinoma) with a total of 935 patients13–17 19 29–31 were enrolled in this meta-analysis according to the criteria. Additionally, the basic information and characteristics of each trial were summarised in tables 1 and 2.…”

Section: Resultsmentioning

confidence: 99%

“…As a newly identified lncRNA, LINC00460 has been reported to be involved in cancer cell proliferation, migration, invasion, apoptosis and EMT 14 15 31. In the past studies, most of researchers demonstrated that overexpression of LINC00460 may promote cancer progression and predict poor prognosis of patients with cancer, and they even considered LINC00460 as a new biomolecular marker for improving clinical cancer therapy and outcomes.…”

Section: Discussionmentioning

confidence: 99%

Prognostic significance of LINC00460 overexpression in solid tumours: a meta-analysis

Dai

Zhang

et al. 2020

Postgraduate Medical Journal

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The prognostic value of long intergenic non-protein coding RNA 460 (LINC00460) overexpression in human solid malignant tumours remains unclear. Therefore, we conducted the meta-analysis to systematically review and assess the evidence for the correlation between LINC00460 overexpression and clinicopathological features and overall survival (OS) of patients with solid malignant tumour. An electronic search of PubMed, EMBASE, Web of Science, CNKI, Cochrane Library, Chinese Biological Medical Literature database and WanFang database was applied to select eligible articles. Pooled ORs or HRs with their 95% CIs were calculated to estimate the effects. 9 eligible studies with a total of 935 patients were enrolled in this meta-analysis. The results revealed that high LINC00460 expression was associated with positive lymph node metastasis (positive vs negative: OR=2.97, 95% CI 1.74 to 5.05, p=0.812), advanced tumour-node-metastasis stage (III+IV vs I+II: OR=2.82, 95% CI 1.64 to 4.85, p=0.193) and poorer differentiation (high vs low: OR=0.60, 95% CI 0.36 to 0.99, p=0.569). Additionally, the overexpression of LINC00460 could predict a poorer OS (HR=1.57, 95% CI 1.39 to 1.77) and the shorter disease-free survival (HR=2.32, 95% CI 1.25 to 4.31). Furthermore, according to subgroup analysis and meta-regression results, the heterogeneity of current meta-analysis may be attributed to the differences of cancer type and follow-up months. High expression of LINC00460 could predict poor prognosis in patients with solid malignant tumour. LINC00460 may serve as potential prognostic biomarker for clinical outcomes in various human solid malignant tumours.

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Downregulation of long noncoding RNA LINC00460 expression suppresses tumor growth in vitro and in vivo in gastric cancer

Cited by 23 publications

References 16 publications

<p>Long noncoding RNA SNHG16 silencing inhibits the aggressiveness of gastric cancer via upregulation of microRNA-628-3p and consequent decrease of NRP1</p>

<p>Long noncoding RNA SNHG16 silencing inhibits the aggressiveness of gastric cancer via upregulation of microRNA-628-3p and consequent decrease of NRP1</p>

Long noncoding RNA LINC00460 conduces to tumor growth and metastasis of hepatocellular carcinoma through miR-342-3p-dependent AGR2 up-regulation

Prognostic significance of LINC00460 overexpression in solid tumours: a meta-analysis

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