Downscaling the in vitro test of fungal bioremediation of polycyclic aromatic hydrocarbons: methodological approach

Abstract

The miniaturization and optimization of a white rot fungal bioremediation experiment is described in this paper. The optimized procedure allows determination of the degradation kinetics of anthracene. The miniaturized procedure requires only 2.5 ml of culture medium. The experiment is more precise, robust, and better controlled comparing it to classical tests in flasks. Using this technique, different parts, i.e., the culture medium, the fungi, and the cotton seal, can be analyzed. A simple sample preparation speeds up the analytical process. Experiments performed show degradation of anthracene up to approximately 60% by Irpex lacteus and up to approximately 40% by Pleurotus ostreatus in 25 days. Bioremediation of anthracene by the consortium of I. lacteus and P. ostreatus shows the biodegradation of anthracene up to approximately 56% in 23 days. At the end of the experiment, the surface tension of culture medium decreased comparing it to the blank, indicating generation of surfactant compounds.

Biodegradation of polycyclic aromatic hydrocarbons (PAHs) by fungal enzymes: A review

“…Likewise, the growth of fungi is inhibited in highly contaminated soils and overall remediation by fungi is extremely slow, needing many days or even more than a month as reported by Drevinskas et al, (2016). In this sense Balaji et al, (2014) also tested different carbon sources for lipase production by P. chrysogenum and L. theobromae and cellulose in M. racemosus and sucrose induced the highest activity in these species.…”

Total Petroleum Hydrocarbon Degradation by Endophytic Fungi from the Ecuadorian Amazon

“…Fungi are used due to their particular metabolic capacities, which mediate the transformation of a wide variety of organic compounds to less complex compounds that are later incorporated into their metabolisms [19] [20]; these processes are facilitated due to the characteristics of their complex enzymatic system and their vigorous hyphae growth [15] [19] [21]. Thus, they offer an undeniable potential for their use in bioremediation processes, accordingly, the study of the diversity and identification of species and fungal strains of biotechnological utility should be a priority in an avid field of constant innovation such In recent years, endophytic fungi have generated major attention in the scientific community due to their wide diversity and particular metabolic capacities [22].…”

Design and applications of a neural networks assisted portable liquid surface tensiometer

“…the determination of the growth medium of fungi, previous results were confirmed. I. lacteus produced more surface tension modifying compounds than P. ostreatus [13]. The equivalent concentration of SDS for the I. lacteus growth medium was 3.6 mM, and for P. ostreatus it was 0.4 mM.…”

“…The fungi were grown in a miniaturized format according to the previously optimized procedure [13]. In a 10 ml gas chromatography vial, 2.5 ml of the growth medium (0.6% malt extract) was added.…”

“…In the previous investigations, white-rot fungi -Irpex lacteus -reduced the surface tension of the growth medium. The fungi also degraded a significant amount of anthracene which only dissolves in surfactant-containing water [13]. Consequently, the need for portable and autonomous surface tension measurement systems is undoubted.…”