Downstream‐Processing mit Membranadsorbern zur Isolierung nativer Proteinfraktionen aus Kartoffelfruchtwasser

Polysialic acid (polySia) is a carbohydrate polymer of varying chain length. It is a promising scaffold material for tissue engineering. In this work, high chain length polySia was produced by an E. coli K1 strain in a 10 L bioreactor in batch-and fed-batch mode, respectively. A new downstream process for polySia is presented, based on membrane adsorber technology and use of inorganic anion exchanger. These methods enable the replacement of precipitation steps, such as acetone-, cetavlon-and ethanol-precipitation of the already established purification process. The purification process was simplified, while process efficiency and product qualities were improved. The overall yield of polySia from a 10 L batch cultivation process was 61 % and for 10 L fed-batch cultivation process 40 % with an overall purity of 98 %. The endotoxin content was determined to be negligible (14 EU mg -1 ). Th e ma i n advantage of this new downstream process is that polySia with high chain length > 130 DP (degree of polymerization) can be obtained. In fed-batch cultivation chain lengths up to 160 DP were obtained.

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“…The elution was performed using 1 M NaCl solution. The membrane purification was performed in a pilot plant (see Supporting information, ).…”

Section: Methodsmentioning

confidence: 99%

Downstream processing of high chain length polysialic acid using membrane adsorbers and clay minerals for application in tissue engineering

Bice

Celik

Wolff

et al. 2012

Engineering in Life Sciences

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“…This aqueous by-product with approximately 5% dry matter mainly contains proteins (~35% of dm), Sustainability 2017, 9, 1492 9 of 46 sugar (~35% of dm), minerals (~20% of dm), organic acids (~4% of dm) and other components [122]. The maximum concentration of toxic glycoalkaloids (α-solanin, α-chaconin) amounts up to 100 ppm which is far below the permitted level in food products [123,124]. The potato protein predominantly consists of patatin, protease inhibitors, high-molecular-weight proteins and is rich in in lysine (7.18%) and methionine (1.06%) [125,126].…”

Section: Protein Extraction From Potato Fruit Juicementioning

confidence: 99%

Processing, Valorization and Application of Bio-Waste Derived Compounds from Potato, Tomato, Olive and Cereals: A Review

Fritsch

Stäbler

Happel³

et al. 2017

Sustainability

157

102

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Abstract:The vast and ever-growing amount of agricultural and food wastes has become a major concern throughout the whole world. Therefore, strategies for their processing and value-added reuse are needed to enable a sustainable utilization of feedstocks and reduce the environmental burden. By-products of potato, tomato, cereals and olive arise in significant amounts in European countries and are consequently of high relevance. Due to their composition with various beneficial ingredients, the waste products can be valorized by different techniques leading to economic and environmental advantages. This paper focuses on the waste generation during industrial processing of potato, tomato, cereals and olives within the European Union and reviews state-of-the-art technologies for their valorization. Furthermore, current applications, future perspectives and challenges are discussed.

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“…Einen neuen Schwerpunkt in der Berichterstattung in der CIT bilden insbesondere zu Beginn des Jahrzehnts Publikationen im Bereich der Säugerzellkultivierung. Während die in den 70er Jahren durch Köhler und Milstein [119] [128], der Ultrafiltration in der pharmazeutischen Industrie [129], der NADPH-Herstellung im Enzym-Membran-Reaktor [130], Maßstabsverkleinerungen von Filtrationen [131] und der Isolierung nativer Proteinfraktionen aus Kartoffelwasser mithilfe von Membranadsorbern [132]. Einen interessanten Ü berblick zum Stand der Membrantechnik des beginnenden 21.…”

Section: Introductionunclassified

90 Years CIT – 90 Years Development in Biotechnology and Bioprocess Engineering

Akermann

Bold

Mehring

et al. 2018

Chemie Ingenieur Technik

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90 Jahre Chemie Ingenieur Technik (CIT) spiegeln wesentliche Meilensteine der Entwicklung der Biotechnologie und Bioprozesstechnik wider. In diesem Artikel werden einige der wichtigsten Entwicklungen vorgestellt und die Ausbildung von Trends in der Bioverfahrenstechnik nachgezeichnet. Beim Lesen der älteren Artikel lässt sich feststellen, dass einige der aktuellen Forschungsgebiete durchaus nicht ganz so neu sind, sondern vielversprechende Ansätze auch früher schon bearbeitet wurden.

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Downstream‐Processing mit Membranadsorbern zur Isolierung nativer Proteinfraktionen aus Kartoffelfruchtwasser

Abstract: Bei der Stärkeproduktion entstehendes Kartoffelfruchtwasser besitzt mit 2 -3 % einen

Cited by 12 publications

References 13 publications

Downstream processing of high chain length polysialic acid using membrane adsorbers and clay minerals for application in tissue engineering

Downstream processing of high chain length polysialic acid using membrane adsorbers and clay minerals for application in tissue engineering

Processing, Valorization and Application of Bio-Waste Derived Compounds from Potato, Tomato, Olive and Cereals: A Review

90 Years CIT – 90 Years Development in Biotechnology and Bioprocess Engineering

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