Doxorubicin delivery enhanced by electroporation to gastrointestinal adenocarcinoma cells with P-gp overexpression

Kulbacka, Julita; Daczewska, Małgorzata; Dubińska–Magiera, Magda; Choromańska, Anna; Rembiałkowska, Nina; Surowiak, Paweł; Kulbacki, Marek; Kotulska, Małgorzata; Saczko, Jolanta

doi:10.1016/j.bioelechem.2014.03.013

Cited by 30 publications

(27 citation statements)

References 31 publications

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“…Among many compounds tested, that is, doxorubicin, 36 ruthenium (III) compound, 37 5-fluorouracil, 38 phthalocyanines, 39 photofrin, 40 curcumin, 41 the trans-platinum analogue trans-[PtCl 2 (3-hmpy) 2 ], 42 oxaliplatin, 43 betulinic acid, 44,45 oat b-glucan, 46 cyanine IR-775, 47 catechin, 48 and sodium decahydrodecaborate, 49 only calcium electroporation was extensively studied. [50][51][52][53][54][55][56][57][58] Calcium electroporation effectively causes tumor necrosis, leaving the normal tissue less affected, making it a very promising compound for further clinical evaluation as it does not belong to the antineoplastic drugs, thus precautions related to antineoplastic drugs regarding storage, handling, disposal, and toxicity and mutagenicity are not an issue for calcium electroporation.…”

Section: Recent Advances In Electrochemotherapy 205mentioning

confidence: 99%

Recent Advances in Electrochemotherapy

Čemažar

Serša

2019

Bioelectricity

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Electrochemotherapy is gaining recognition as an effective local therapy that uses systemically or intratumorally injected bleomycin or cisplatin with electroporation as a delivery system that brings drugs into the cells to exert their cytotoxic effects. Preclinical work is still ongoing, testing new drugs, seeking the best treatment combination with other treatment modalities, and exploring new sets of pulses for effective tissue electroporation. The applications of electrochemotherapy are being fully exploited in veterinary oncology, where electrochemotherapy, because of its simple execution, has a relatively good cost-benefit ratio and is used in the treatment of cutaneous tumors. In human oncology, electrochemotherapy is fully recognized as a local therapy for cutaneous tumors and metastases. Its effectiveness is being explored in combination with immunomodulatory drugs. However, the development of electrochemotherapy is directed into the treatment of deepseated tumors with a percutaneous approach. Because of the vast number of reports, this review discusses the articles published in the past 5 years.

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Section: Recent Advances In Electrochemotherapy 205mentioning

confidence: 99%

Recent Advances in Electrochemotherapy

Čemažar

Serša

2019

Bioelectricity

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“…On the other hand, when cells were subjected to the action of pulsed electric field, the effects were exactly opposite. Remarkably, multidrugresistant cell lines appear to be more sensitive to higher voltage than sensitive cancer cells and normal cell lines which has been observed also during electrochemotherapy (Gehl, 2005;Kulbacka et al, 2014), irreversible electroporation (Neal et al, 2011) and on 3D culture models (Frandsen et al, 2015). Frandsen et al (2016) explains that phenomenon by the more effective membrane repair in normal, primary cells in comparison to the resistant cancer cells (Frandsen et al, 2016).…”

Section: Discussionmentioning

confidence: 85%

“…The final concentrations of ethanol in samples was up to ~1%, which was proven to be non-toxic for cells prior to the study. For EP experiments catechin was diluted to 10 µM with EP buffer of a composition described previously (Kulbacka et al, 2014). Cis-Diamineplatinum(II) dichloride (Sigma, cat.…”

Section: Methodsmentioning

confidence: 99%

“…Electroporation protocol. Prior to the electroporation (preceded or not by the catechin preincubation) cells were collected with trypsin and placed in dedicated cuvettes containing EP buffer of low conductivity (Kulbacka et al, 2014). Then, for some cells drugs (cisplatin or mix of catechin/cisplatin) were added and part of the samples were electroporated.…”

Section: Methodsmentioning

confidence: 99%

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The favourable effect of catechin in electrochemotherapy in human pancreatic cancer cells

et al. 2018

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Until recently, green tea polyphenols were considered strong antioxidants. However, the latest reports have revealed that bioflavonoids can play a multiple role in anticancer therapy, including the inhibition of cell proliferation and generation of the oxidative stress in a dose-dependent manner. The presented research was designed to examine the potential of the green tea (±)-catechin as a reinforcement of the electrochemotherapy (ECT) with cisplatin in pancreatic cancer in vitro. The study was performed on two cell lines of the pancreatic ductal adenocarcinoma (PDA) - parental EPP85-181P and multidrug-resistant EPP85-181RNOV. Prior to the ECT protocol the cells were preincubated with high or low concentration of catechin for 2 or 24 hours, respectively. We assessed the influence of preincubation on the cisplatin toxicity with and without electroporation (EP), the electrosensitivity of PDA cell lines and the uptake of the daunorubicin and propidium iodide. Additionally, we evaluated the antioxidative properties of catechin by the measurement of the ROS-related fluorescence and the immunoreactivity of the oxidative stress-related enzymes superoxide dismutase (SOD2) and glutathione S-transferase (GST). We found that co-treatment with catechin can firmly enhance the efficacy of electroporation with cisplatin in vitro. More favorable effect was obtained for 2-hour incubation, which indicates the involvement of the transcriptional-independent mechanisms of catechin action. The effect may be partially explained by the increased oxidative stress level, which was higher in multidrug-resistant cells. However, further studies on cisplatin-catechin interplay and the thorough examination of the catechin-cell membrane interaction need to be performed.

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“…The electropermeabilization technology can be effectively applied in transport of molecules, such as chemotherapeutics in electrochemotherapy (Mir et al 1992 , 2003 , 2014 ; Sersa et al 1998 , 2008 , 2009 ; Heller et al 2002 ; Čemažar et al 1998a , 1998b ; Kulbacka et al 2014 ; Saczko et al 2014 ; Vasquez et al 2014 ; Campana et al 2014 ; Yarmush et al 2014 ; Cadossi et al 2014 ; Miklavcic et al 2014 ) or nucleic acids in gene therapy (Jaroszeski et al 1995 , 1999 , 2000 , 2004 ; Heller et al 1996 , 2001 , 2005 , 2010 ; Ugen and Heller 2003 ; Gillbert et al 2002 ; Aihara et al 1998 ; Miyazaki and Aihara 2002 ; Rols et al 1998 ; Gibot and Rols 2015 ; Faurie et al 2003 ; Orio et al 2013 ; Forde et al 2014 ; Emr et al 2014 ; Kalli et al 2014 ; Gehl 2014 ; Takei 2014 ; Hu and Li 2014 ). In the case of electrochemotherapy, electropores appearing in the cell membrane after application of the electric field enable transport of small non-lipophilic drug molecules into the cell.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Millisecond Pulsed Electric Fields (msPEF) on Intracellular Drug Transport with Negatively Charged Large Nanocarriers Made of Solid Lipid Nanoparticles (SLN): In Vitro Study

et al. 2016

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Drug delivery technology is still a dynamically developing field of medicine. The main direction in nanotechnology research (nanocarriers, nanovehicles, etc.) is efficient drug delivery to target cells with simultaneous drug reduction concentration. However, nanotechnology trends in reducing the carrier sizes to several nanometers limit the volume of the loaded substance and may pose a danger of uncontrolled access into the cells. On the other hand, nanoparticles larger than 200 nm in diameter have difficulties to undergo rapid diffusional transport through cell membranes. The main advantage of large nanoparticles is higher drug encapsulation efficiency and the ability to deliver a wider array of drugs. Our present study contributes a new approach with large Tween 80 solid lipid nanoparticles SLN (i.e., hydrodynamic GM-SLN—glycerol monostearate, GM, as the lipid and ATO5-SLNs—glyceryl palmitostearate, ATO5, as the lipid) with diameters DH of 379.4 nm and 547 nm, respectively. They are used as drug carriers alone and in combination with electroporation (EP) induced by millisecond pulsed electric fields. We evaluate if EP can support the transport of large nanocarriers into cells. The study was performed with two cell lines: human colon adenocarcinoma LoVo and hamster ovarian fibroblastoid CHO-K1 with coumarin 6 (C6) as a fluorescent marker for encapsulation. The biological safety of the potential treatment procedure was evaluated with cell viability after their exposure to nanoparticles and EP. The EP efficacy was evaluated by FACS method. The impact on intracellular structure organization of cytoskeleton was visualized by CLSM method with alpha-actin and beta-tubulin. The obtained results indicate low cytotoxicity of both carrier types, free and loaded with C6. The evaluation of cytoskeleton proteins indicated no intracellular structure damage. The intracellular uptake and accumulation show that SLNs do not support transport of C6 coumarin. Only application of electroporation improved the transport of encapsulated and free C6 into both treated cell lines.Electronic supplementary materialThe online version of this article (doi:10.1007/s00232-016-9906-1) contains supplementary material, which is available to authorized users.

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Doxorubicin delivery enhanced by electroporation to gastrointestinal adenocarcinoma cells with P-gp overexpression

Cited by 30 publications

References 31 publications

Recent Advances in Electrochemotherapy

Recent Advances in Electrochemotherapy

The favourable effect of catechin in electrochemotherapy in human pancreatic cancer cells

The Effect of Millisecond Pulsed Electric Fields (msPEF) on Intracellular Drug Transport with Negatively Charged Large Nanocarriers Made of Solid Lipid Nanoparticles (SLN): In Vitro Study

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