DRACULA2, a dynamic nucleoporin with a role in the regulation of the shade avoidance syndrome in Arabidopsis

Gallemí, Marçal; Galstyan, Anahit; Paulišić, Sandi; Then, Christiane; Ferrández‐Ayela, Almudena; Lorenzo‐Orts, Laura; Roig‐Villanova, Irma; Wang, Xuewen; Micol, José Luis; Ponce, Marı́a Rosa; Devlin, Paul F.; Martínez‐Garcia, Jaime F.

doi:10.1242/dev.130211

Cited by 31 publications

(45 citation statements)

References 53 publications

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“…Two light intensity/shade responsive genes were detected to be up-regulated under SHADE in the northern population – DRACULA2 (DRA2) involved in hypocotyl elongation and control of shade induced gene expression (Gallemi et al , 2016) and PHYTOCHROME RAPIDLY REGULATED 2 ( PAR2 ) that encodes a protein which is up-regulated under shade and acts as negative regulator of shade avoidance response (Roig-Villanova et al , 2006). One light-regulated gene was detected to be up-regulated in the southern population - TEOSINTE BRANCHED 1, CYCLOIDEA AND PCF TRANSCRIPTION FACTOR 2 ( TCP2 ); TCP2 is a positive regulator of photomorphogenesis and is involved in response to blue light (He et al , 2016).…”

Section: Resultsmentioning

confidence: 99%

Molecular signatures of local adaptation to light in Norway Spruce: role of lignin mediated immunity

2020

Preprint

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Study of natural variation is an efficient method to elucidate how plants adapt to local climatic conditions, a key process for the evolution of a species. However, it is challenging to determine the genetic basis of adaptive variation especially in forest trees which have large and complex genomes. Norway spruce is a shade tolerant conifer in which the requirement of far-red light for growth increases latitudinally northwards. In the current work, hypocotyl-length followed a latitudinal cline in response to SHADE (low red:far-red ratio). RNA-sequencing revealed differential gene expression in response to SHADE, between a southern and a northern natural population in Sweden. Exome capture included analysis of uniquely large data set (1654 trees) that revealed missense variations in coding regions of nine differentially expressed candidate genes, which followed a latitudinal cline in allele and genotype frequencies. These genes included five transcription factors involved in vital processes like bud-set/bud-flush, lignin pathway and cold acclimation, and other genes that take part in cell-wall remodeling, secondary cell-wall thickening, response to starvation and immunity. Findings from this work primarily suggests that the northern populations of Norway spruce are better adapted towards disease resistance under shade by up-regulation of lignin pathway that is linked to immunity and it forms concrete basis for local adaptation to light quality in Norway spruce, one of the most economically important conifer tree species in Sweden.

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Section: Resultsmentioning

confidence: 99%

Molecular signatures of local adaptation to light in Norway Spruce: role of lignin mediated immunity

2020

Preprint

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“…Nups that were reported in the past for one phenotype or pathway are often later associated with another pathway, either related or unrelated, as is the case for Nup88 and Nup98 in Genenncher et al (2016) and Gallemí et al (2016), respectively. Nup88-also called MOS7-was known as a contributor to the autoimmunity of the suppressor of npr1-1, constitutive1 mutant (Cheng et al, 2009).…”

Section: The Nuclear Pore and Nuclear Transportmentioning

confidence: 99%

“…Nup98a is also known as DRACULA2, based on a mutant screen for genes involved in shade avoidance syndrome (SAS). In nup98a mutant alleles, the SAS response is diminished (Gallemí et al, 2016). In addition to nup98a, several mutant alleles of NUP160 and NUP96 (scaffold Nups) as well as NUP58, NUP54, and NUP62 (like Nup98a, predicted to be peripheral Nups) were also tested.…”

Section: The Nuclear Pore and Nuclear Transportmentioning

confidence: 99%

Dynamic Changes in Plant Nuclear Organization in Response to Environmental and Developmental Signals

et al. 2017

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ORCID ID: 0000-0002-4141-5400 (I.M.).In both plants and animals, the nucleus acts as an organizing center for the changes that occur during an organism's life cycle, whether as part of a developmental program or in response to environmental factors. Here, we cover recent research that explores roles of the nucleus other than gene expression in light response, fertilization, plant-pathogen interactions, bacterial symbiotic events, and hormone signaling. New strides have been made in understanding subnuclear organization, how nuclear organization responds to different environments and developmental stages, and how the cytoplasm-nucleoplasm connections are made that allow these responses. We further highlight new tools that have been developed to study dynamic changes in nuclear organization.The nucleus is compartmentalized by a double membrane called the nuclear envelope (NE), which consists of the outer nuclear membrane (ONM), the inner nuclear membrane (INM), and the embedded nuclear pore complexes (NPCs). The NPC is the primary pathway of macromolecular transport between the nucleus and the cytoplasm and is conserved throughout eukaryotes. The ONM and INM are populated by a variety of membrane-associated proteins, and in particular the inner membrane hosts a specific subset of proteins (Starr and Fridolfsson, 2010;Meier et al., 2017). In addition to functioning in nuclear morphology, chromatin attachment, and likely signal transduction, plant NE-associated proteins are involved in nuclear positioning and movement within the larger cellular context (Griffis et al., 2014). While plant nuclear ultrastructure reveals an inner nuclear membrane-associated meshwork similar to the animal lamina, plant genomes do not encode obvious lamin homologs. Instead, plant-specific long coiled-coil proteins with structural similarity to animal lamins might contribute to this meshwork. Plants also lack centrosomes, and the NE plays a role as the microtubuleorganizing center at the onset of mitosis. NE proteins functioning as calcium channels are involved in perinuclear calcium oscillations, which are an important step in the establishment of plant-symbiont interactions (recently reviewed in Meier et al., 2017).While the nuclear periphery is emerging as an essential organizing platform for the nucleus, intranuclear functional and structural organization might also originate from independent self-assembly processes, with the most conspicuous manifestation of these processes revealed as the nucleolus. New methods are now robustly adapted for plants to reveal greater details of functional nuclear and nucleolar organization. Due to the increasing realization that spatial organization is a crucial part of biological function at the subcellular level, many of these aspects have been recently reviewed

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“…Nup98 is a mobile and peripheral FG (for Phe-Gly domain) nucleoporin and spans from the nucleus to cytoplasmic of the central channel of the NPC [4,5]. Arabidopsis thaliana Nup98a (also known as DRA2) is also found in different subcellular locations [6]. Nup98 is involved in regulation of cargo export and import, gene expression, transcriptional memory, and multiple developmental processes in animals and yeast [4][5][6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Two Nucleoporin 98 homologous genes jointly participate in the regulation of starch degradation to enhance growth in Arabidopsis

Xiao

Jiang

Huang

et al. 2020

Preprint

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Background: Starch is synthesized during the day for temporary storage in leaves and then degraded during the subsequent night to support plant growth and development. Impairment of starch degradation leads to stunted growth, even senescence and death. The nuclear pore complex is involved in many cellular processes, but its relationship with starch degradation is unclear until now. We previously identified two Nucleoporin98 ( Nup98 ) genes ( Nup98a and Nup98b ) redundantly regulated flowering through CONSTANS ( CO ) independent pathway. in Arabidopsis thaliana. The nup98a nup98b double mutant also showed severe senescence phenotypes. Results: We found that Nucleoporin 98 ( Nup98 ) participated in the regulation of sugar metabolism in leaves and in turn is involved in senescence regulation in Arabidopsis . We show that Nup98a and Nup98b redundantly function in the different steps of starch degradation, the nup98a nup98b double mutant accumulates more starch than wild type and has a severe early senescence phenotype compared to wild type. The expression of marker genes related to starch degradation was impaired in the nup98a nup98b double mutant, and indicator genes of carbon starvation and senescence expressed earlier in the nup98a nup98b double mutant than that in wild type plants, suggesting abnormality of energy metabolism was the cause of senescence of the nup98a nup98b double mutant. Addition of sucrose to the growth medium can rescue early senescence phenotype of the nup98a nup98b mutant. Conclusions: Our results provided a line of evidence on a novel role of the nuclear pore complex in energy metabolism related to growth and development, whereas Nup98 functioned in starch degradation conferring growth regulation in Arabidopsis .

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DRACULA2, a dynamic nucleoporin with a role in the regulation of the shade avoidance syndrome in Arabidopsis

Cited by 31 publications

References 53 publications

Molecular signatures of local adaptation to light in Norway Spruce: role of lignin mediated immunity

Molecular signatures of local adaptation to light in Norway Spruce: role of lignin mediated immunity

Dynamic Changes in Plant Nuclear Organization in Response to Environmental and Developmental Signals

Two Nucleoporin 98 homologous genes jointly participate in the regulation of starch degradation to enhance growth in Arabidopsis

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