Draft genome analysis of a multidrug-resistant Pseudomonas aeruginosa CMPL223 from hospital wastewater in Dhaka, Bangladesh

Khan, Md Abu Sayem; Nahid, Zahidul Islam; Miah, Md Ibrahim; Rahman, Sabita Rezwana

doi:10.1016/j.jgar.2022.06.028

Cited by 6 publications

(6 citation statements)

References 12 publications

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“…However, the study isolates showed high to moderate level (44.0 to 78.0%) sensitivity against Amikacin, Gentamicin and Ciprofloxacin. These results corroborated with several earlier studies that investigated antimicrobial susceptibility profile in P. aeruginosa isolated from clinical samples [ 4 ], environmental samples [ 38 ] and poultry samples [ 43 ]. Consistent findings across various studies examining the antimicrobial susceptibility profile of P .…”

Section: Discussionsupporting

confidence: 92%

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Characterization of β-lactamase and virulence genes in Pseudomonas aeruginosa isolated from clinical, environmental and poultry sources in Bangladesh

Islam,

Ferdous,

Hoque

et al. 2024

PLoS ONE

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The emergence and spread of multidrug-resistant pathogens like Pseudomonas aeruginosa are major concerns for public health worldwide. This study aimed to assess the prevalence of P. aeruginosa in clinical, environmental, and poultry sources in Bangladesh, along with their antibiotic susceptibility and the profiling of β-lactamase and virulence genes using standard molecular and microbiology techniques. We collected 110 samples from five different locations, viz., BAU residential area (BAURA; n = 15), BAU Healthcare Center (BAUHCC; n = 20), BAU Veterinary Teaching Hospital (BAUVTH; n = 22), Poultry Market (PM; n = 30) and Mymensingh Medical College Hospital (MCCH; n = 23). After overnight enrichment in nutrient broth, 89 probable Pseudomonas isolates (80.90%) were screened through selective culture, gram-staining and biochemical tests. Using genus- and species-specific PCR, we confirmed 22 isolates (20.0%) as P. aeruginosa from these samples. Antibiogram profiling revealed that 100.0% P. aeruginosa isolates (n = 22) were multidrug-resistant isolates, showing resistance against Doripenem, Penicillin, Ceftazidime, Cefepime, and Imipenem. Furthermore, resistance to aztreonam was observed in 95.45% isolates. However, P. aeruginosa isolates showed a varying degree of sensitivity against Amikacin, Gentamicin, and Ciprofloxacin. The blaTEM gene was detected in 86.0% isolates, while blaCMY, blaSHV and blaOXA, were detected in 27.0%, 18.0% and 5.0% of the P. aeruginosa isolates, respectively. The algD gene was detected in 32.0% isolates, whereas lasB and exoA genes were identified in 9.0% and 5.0% P. aeruginosa isolates. However, none of the P. aeruginosa isolates harbored exoS gene. Hence, this study provides valuable and novel insights on the resistance and virulence of circulating P. aeruginosa within the clinical, environmental, and poultry environments of Bangladesh. These findings are crucial for understanding the emergence of β-lactamase resistance in P. aeruginosa, highlighting its usefulness in the treatment and control of P. aeruginosa infections in both human and animal populations.

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Section: Discussionsupporting

confidence: 92%

“…Previous studies in Bangladesh have focused on clinical isolates, healthcare workers’ mobile phones, and even the genomic diversity of MDR P . aeruginosa from burn patients [ 4 , 13 , 38 ]. Therefore, this study was designed to determine the antibiotic resistance profiles and virulence genes distribution in P .…”

Section: Discussionmentioning

confidence: 99%

Characterization of β-lactamase and virulence genes in Pseudomonas aeruginosa isolated from clinical, environmental and poultry sources in Bangladesh

Islam,

Ferdous,

Hoque

et al. 2024

PLoS ONE

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“…However, the study isolates showed high to moderate level (44.0 to 78.0%) sensitivity against Amikacin, Gentamicin and Ciprofloxacin. These results corroborated with several earlier studies that investigated antimicrobial susceptibility profile in P. aeruginosa isolated from clinical samples [4], environmental samples [37] and poultry samples [42]. Consistent findings across various studies examining the antimicrobial susceptibility profile of P. aeruginosa isolated from diverse sources (clinical, environmental, and animal samples) can lend support to the understanding of the AMR patterns and prevalence of MDR strains within this bacterium.…”

Section: Discussionsupporting

confidence: 89%

“…However, sufficient information is unavailable on the environmental distribution of potential pathogenic MDR P. aeruginosa in Bangladesh. Previous studies in Bangladesh have focused on clinical isolates, healthcare workers’ mobile phones, and even the genomic diversity of MDR P. aeruginosa from burn patients [4, 36, 37]. Therefore, this study was designed to determine the antibiotic resistance profiles and virulence genes distribution in P. aeruginosa circulating in different environments of Bangladesh.…”

Section: Discussionmentioning

confidence: 99%

Characterization of β-lactamase and virulence genes inPseudomonas aeruginosaisolated from clinical, environmental and poultry sources in Bangladesh

Islam,

Ferdous,

Hoque

et al. 2023

Preprint

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The emergence and spread of multidrug-resistant pathogens likePseudomonas aeruginosaare major concerns for public health worldwide. This study aimed to investigate the prevalence of circulatingP. aeruginosaisolated from clinical, environmental and poultry sources in Bangladesh, their antibiotic susceptibility, β-lactamase and virulence gene profiling using standard molecular and microbiology techniques. We collected 110 samples from five different locations,viz., BAU residential area (BAURA; n=15), BAU Healthcare Center (BAUHCC; n = 20), BAU Veterinary Teaching Hospital (BAUVTH; n=22), Poultry Market (PM; n=30) and Mymensingh Medical College Hospital (MCCH; n=23). After overnight enrichment in nutrient broth, 89 probablePseudomonasisolates (80.90%) were screened through selective culture, gram-staining and biochemical tests. Using genus- and species-specific PCR, we confirmed 22 isolates (20.0%) asP. aeruginosafrom these samples. Antibiogram profiling revealed that 100.0%P. aeruginosaisolates (n = 22) were multidrug-resistant isolates, showing resistance against Doripenem, Penicillin, Ceftazidime, Cefepime, and Imipenem. Furthermore, resistance to aztreonam was observed in 95.45% isolates. However,P. aeruginosaisolates showed a varying degree of sensitivity against Amikacin, Gentamicin, and Ciprofloxacin. TheblaTEMgene was detected in 86.0% isolates, whileblaCMY,blaSHVandblaOXA,were detected in 27.0%, 18.0% and 5.0% of theP. aeruginosaisolates, respectively. ThealgDgene was detected in 32.0% isolates, whereaslasBandexoAgenes were identified in 9.0% and 5.0%P. aeruginosaisolates. However, none of theP. aeruginosaisolates harboredexoSgene. Thus, this study provides novel and important data on the resistance and virulence ofP. aeruginosacurrently circulating in clinical, environmental and poultry environment of Bangladesh. These data provide important insights into the emergence of β-lactamase resistance inP. aeruginosa, highlighting its usefulness in the treatment and control ofP. aeruginosainfections in both humans and animals.

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“…1 Due to their prominent virulence factors and multidrug resistance attributes, the infections due to this bacterium are very severe. [2][3][4][5] Burn deaths have been associated with Pseudomonas infection in over 75% of cases. 6,7 Biofilm and basal protease enzyme are the medically important virulence factors responsible for the invasiveness and disease transmission by this bacterium, especially in the resistant strains.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Sub-Inhibitory Concentration of Clove Essential Oil on the Expression of Pseudomonas aeruginosa Virulence genes

2024

TJNPR

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A total of seven genes encode the proteins involved in Pel exopolysaccharide synthesis; PelA, PelB, PelC, PelD, PelE, PelF, and PelG. 17,18 The severity of the infection caused by P. aeruginosa is not determined only by virulence factors, but also by the presence of multidrug-resistant strains. Multidrug-resistant P. aeruginosa are associated with high mortality worldwide. 19 Disease such as persistent cystic fibrosis caused by multidrug-resistant P. aeruginosa spreads rapidly from patients to patients leading to a high possibility of an outbreak, and therefore, the need for extreme caution in the management of such infection. 20,21 The role of plants' essential oils like Syzygium aromaticum essential oil commercially called clove essential oil (CEO) in the treatment of bacterial infections has been emphasized in several studies. [22][23][24][25][26][27] The influence of the components of CEO on several bacterial virulence factors have been evaluated. These include factors that are controlled by Rhl and Las genes, including protease, LasB, swarming, and the production of exopolysaccharides and proteins like pyocyanins and chitinases. 28 According to the American Food and Drug Administration (FDA), CEO is harmless when used in foods and for medical treatments. 29 The present study aims to investigate the effect of clove essential oil on pelF and aprA expression in multidrug-resistant strains of P. aeruginosa. Materials and Methods Plant collection and identificationClove (Syzygium aromaticum) flower buds were collected from Baghdad city national parks and gardens, from the middle of April to the end of May, 2022.

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Draft genome analysis of a multidrug-resistant Pseudomonas aeruginosa CMPL223 from hospital wastewater in Dhaka, Bangladesh

Cited by 6 publications

References 12 publications

Characterization of β-lactamase and virulence genes in Pseudomonas aeruginosa isolated from clinical, environmental and poultry sources in Bangladesh

Characterization of β-lactamase and virulence genes in Pseudomonas aeruginosa isolated from clinical, environmental and poultry sources in Bangladesh

Characterization of β-lactamase and virulence genes inPseudomonas aeruginosaisolated from clinical, environmental and poultry sources in Bangladesh

The Effect of Sub-Inhibitory Concentration of Clove Essential Oil on the Expression of Pseudomonas aeruginosa Virulence genes

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