Draft genome sequence data of a chromium reducing bacterium, Bacillus licheniformis strain KNP

Arora, Pankaj; Mishra, Rahasya Mani; Omar, Rishabh Anand; Saroj, Raj Shekhar; Srivastava, Alok Kumar; Garg, Sanjay; Singh, Vijay Pal

doi:10.1016/j.dib.2020.106640

Cited by 5 publications

(3 citation statements)

References 18 publications

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“…On the other hand, B. paralicheniformis KNPhave shown a good capacity to reduce chromate through three possible chromate efflux transporters. When analyzing the genome of strain KNP, genes that code for proteins involved in the reduction and resistance to As were also detected, such as thioredoxin, arsA, and arsB (Arora et al, 2021). Other strains of B. paralicheniformis have also been isolated from Espinazo Hot springs in Nuevo Leon, México (Silva-Salinas et al, 2021), Morocco (Maski et al, 2021), and Algeria (Benammar et al, 2020).…”

Section: Resultsmentioning

confidence: 99%

Genome mining, phylogenetic, and functional analysis of arsenic (As) resistance operons in Bacillus strains, isolated from As-rich hot spring microbial mats

Flores

Valencia-Marin

Chávez-Avila

et al. 2022

Preprint

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The geothermal zone of Araró, México, is located within the trans-Mexican volcanic belt, an area with numerous arsenic (As)-rich hot springs. In this study, the draft genome sequence of two endemic Bacillus strains (ZAP17 and ZAP62) from Araró microbial mat hot springs was determined, which were able to grow on arsenate (up to 64 mM) and arsenite (up to 32 mM). Phylogenetic analysis based on 16S rRNA and gyrB sequences, as well as genome sequence analysis based on average nucleotide identity (>96%) and digital DNA–DNA hybridization (>70%), indicated that these strains belong to the Bacillus paralicheniformis ZAP17 and Bacillus altitudinis ZAP62. Furthermore, through genome mining, it was identified two arsenic resistance operons, arsRBC, and arsRBCDA in both strains as potential determinants of arsenic (As) resistance. Predicted ArsA (arsenial pump-driving ATPase), ArsB (Arsenical efflux pump protein), ArsC (Arsenate reductase), ArsD (Arsenical efflux pump protein) and ArsR (Metalloregulator/ars operon repressor) proteins, clearly grouped with their respective clades corresponding to other characterized bacterial species, mainly Firmicutes. To further evaluate the functionality of the ars operons in ZAP17 and ZAP62 strains, our results showed that arsRBC and arsRBCDA genes were expressed in the presence of arsenite (III). Finally, the presence of ars operons in the genome of Bacillus species residing in As-rich environments, such as the Araró hot springs, might be a potential mechanism to survive under such harsh conditions, as well as to design sustainable bioremediation strategies.

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Section: Resultsmentioning

confidence: 99%

Genome mining, phylogenetic, and functional analysis of arsenic (As) resistance operons in Bacillus strains, isolated from As-rich hot spring microbial mats

Flores

Valencia-Marin

Chávez-Avila

et al. 2022

Preprint

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“…The circular genome of B. licheniformis is approximately 4.3 Mbp [9], with 46% GC content [10], over 5000 coding sequences (CDSs), and exhibits slight differences between subspecies [11]. Noteworthy, comparative genomics indicates that B. licheniformis are approximately 84.6% identical to B. subtilis at the nucleotide level [7], which is also indirectly reflected by the relatively close position of the two in the evolutionary tree [12].…”

Section: Updated Genome Information Of B Licheniformismentioning

confidence: 99%

Recent biotechnological advances and future prospective of Bacillus licheniformis as microbial cell factories

Zhang

Shi

et al. 2023

Syst Microbiol and Biomanuf

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Bacillus licheniformis is a characteristic Gram-positive bacterium originally found in soil. This microorganism has long been utilised as a workhorse for production of industrial enzymes or high value-added chemicals. With ever-increasing understanding on this strain and the maturation of the genetic technique, important advances have recently been made in developing B. licheniformis as an excellent chassis cell for synthetic biology. Here, we provide an overview of updated understanding on genome information, anaerobic metabolism, industrial applications of this strain. The state-of-art B. licheniformis genetics, especially its synthetic biology advances in biosensor, expression system and artificial metabolic pathways are illustrated. Finally, perspectives are offered for the limitations and challenges to be addressed to improve B. licheniformis as microbial cell factories.

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“…In recent years, researchers have discovered some bacteria that can remove Cr(VI), including Serratia sp. S2 [3], Bacillus cereus [4], Bacillus licheniformis [5], and Sporosarcina saromensis [6]. Although most studies on Cr(VI) removal by bacteria have focused on strain isolation and cultivation, mechanistic studies on chromium metabolism genes and proteins in bacteria are also being conducted.…”

Section: Introductionmentioning

confidence: 99%

Analysis of the Genome of Chromium-resistant Serratia sp. CM01 and Chromium Metabolism Characteristics of the ChrA1-Srpc Gene

Wang,

Li,

Yin

et al. 2023

Preprint

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Objective: Serratia sp. CM01 is a naturally domesticated strain with chromium (Cr) resistance and Cr(VI) reduction ability isolated from a Cr-enriched environment. The genome of Serratia sp. CM01 was sequenced and analysed to identify key genes involved in chromium metabolism. Corresponding engineered bacteria associated with special metabolic features were constructed to further study the function of these genes and their encoded proteins in chromium metabolism. Methods: The whole genome of Serratia sp. CM01 was sequenced, and genes involved in chromium metabolism were analysed. The ChrA1 and Srpc genes were selected and introduced into E. coli using the prokaryotic expression vector pET-28a. The target proteins were identified using sodium dodecyl sulphate‒polyacrylamide gel electrophoresis (SDS‒PAGE) and Western blotting. Chromium tolerance and removal tests were used to assess the chromium metabolic capabilities of the engineered bacteria. Results: The results of genome sequencing revealed that the genome size of Serratia sp. was 4,902,254 bp, from which 12 genes involved in chromium metabolism were screened. The ChrA1 and Srpc genes were chosen, and three engineered bacteria (eChrA1, eSrpc, and eCS) were successfully constructed. The growth of the three engineered bacteria showed no difference under Cr(VI)-free circumstances (P > 0.05). In the presence of Cr(VI), the viable bacterial cell mount during the stabilization phase was eCS > eChrA1 > eSrpc, and the tolerance and removal rate of Cr(VI) was ranked eCS > eChrA1 > eSrpc. Conclusion: The genome of Serratia sp. CM01 contains genes related to chromium metabolism, such as NemA, ChrA1, Srpc and FieF. The ChrA1 and Srpc gene-encoded proteins confer Cr(VI) resistance to engineered bacteria, but the specific mechanism of chromium resistance remains to be further studied. eChrA1 has a stronger anti-Cr(VI) ability than eSrpc. The eCS-engineered bacterial strain with ChrA1 and Srpcgenes was more resistant to hexavalent chromium, and it has the potential to handle Cr(VI) pollution in the virtual environment.

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Draft genome sequence data of a chromium reducing bacterium, Bacillus licheniformis strain KNP

Cited by 5 publications

References 18 publications

Genome mining, phylogenetic, and functional analysis of arsenic (As) resistance operons in Bacillus strains, isolated from As-rich hot spring microbial mats

Genome mining, phylogenetic, and functional analysis of arsenic (As) resistance operons in Bacillus strains, isolated from As-rich hot spring microbial mats

Recent biotechnological advances and future prospective of Bacillus licheniformis as microbial cell factories

Analysis of the Genome of Chromium-resistant Serratia sp. CM01 and Chromium Metabolism Characteristics of the ChrA1-Srpc Gene

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