Draft Genome Sequence of a Thermophilic Cyanobacterium from the Family
            <i>Oscillatoriales</i>
            (Strain MTP1) from the Chalk River, Colorado

Hallenbeck, Patrick C.; Grogger, Melanie; Mraz, Megan; Veverka, Donald

doi:10.1128/genomea.01571-15

Cited by 6 publications

(4 citation statements)

References 17 publications

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“…The most abundant ASVs belonged to photosynthetic bacteria within the Cyanobacteria and Chloroflexia. Amongst the cyanobacteria the filamentous novel Oscillatoriales cyanobacterium MTP1 (ASV0) (Hallenbeck et al, 2016) was most abundant in mats, whereas in the flocs at highest stress levels it was largely replaced by unicellular Thermosynechococcus elongatus (ASV4) as the dominant oxygenic photoautotroph and this was consistent with our microscopy observations. Other cyanobacteria (ASV45, Geitlerinema, ASV87 and ASV 98 Leptolyngya, and ASV24 Fischerella) occurred at low abundance and their distribution reflected a preference for lower temperatures.…”

Section: Estimation Of Taxonomic Diversity In Mats and Flocssupporting

confidence: 90%

Community structure of thermophilic photosynthetic microbial mats and flocs at Sembawang Hot Spring, Singapore

et al. 2023

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The Sembawang Hot Spring in Singapore lies at the foot of a major regional geological feature called the Bentong-Raub Suture Zone. Amid an extensively managed surface geothermal park, an undisturbed hot spring emerges with source water at 61°C, pH 6.8, and 1 mg/L dissolved sulfide. A small main pool at the source supported orange-green benthic flocs, whereas the outflow channel with gradually less extreme environmental stress supported extensive vivid green microbial mats. Microscopy revealed that cyanobacterial morphotypes were distinct in flocs and mats at several intervals along the environmental gradient, and we describe a spiraling pattern in the oscillatorian cyanobacteria that may reflect response to poly-extreme stress. Estimation of diversity using 16S rRNA gene sequencing revealed assemblages that were dominated by phototrophic bacteria. The most abundant taxa in flocs at 61°C/1 mg/L sulfide were Roseiflexus sp. and Thermosynechococcus elongatus, whilst the mats at 45.7–55.3°C/0–0.5 mg/L sulfide were dominated by Oscillatoriales cyanobacterium MTP1 and Chloroflexus sp. Occurrence of diverse chemoautotrophs and heterotrophs reflected known thermal ranges for taxa, and of note was the high abundance of thermophilic cellulolytic bacteria that likely reflected the large allochthonous leaf input. A clear shift in ASV-defined putative ecotypes occurred along the environmental stress gradient of the hot spring and overall diversity was inversely correlated to environmental stress. Significant correlations for abiotic variables with observed biotic diversity were identified for temperature, sulfide, and carbonate. A network analysis revealed three putative modules of biotic interactions that also reflected the taxonomic composition at intervals along the environmental gradient. Overall, the data indicated that three distinct microbial communities were supported within a small spatial scale along the poly-extreme environmental gradient. The findings add to the growing inventory of hot spring microbiomes and address an important biogeographic knowledge gap for the region.

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Section: Estimation Of Taxonomic Diversity In Mats and Flocssupporting

confidence: 90%

Community structure of thermophilic photosynthetic microbial mats and flocs at Sembawang Hot Spring, Singapore

et al. 2023

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“…Related taxa have been already described for alkaline water bodies, including a serpentinizing ecosystem (OTUs B, D) [ 47 , 52 ]. One was described as extremophilic (thermophilic, heavy metal resistant: OTU A from an enrichment culture of a sample obtained at a thermal source at Chalk Creek, Colorado [ 53 ]), or related to carbonate precipitation (OTU C) [ 54 ]. Two OTUs are exclusively retrieved from non-alkaline creek water ( Chroakolemma -related Leptolyngbyaceae , Pseudanabeaceae Cyanobacterium CENA528).…”

Section: Discussionmentioning

confidence: 99%

Cyanobacterial Mats in Calcite-Precipitating Serpentinite-Hosted Alkaline Springs of the Voltri Massif, Italy

et al. 2020

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(1) Background: Microbial communities in terrestrial, calcifying high-alkaline springs are not well understood. In this study, we investigate the structure and composition of microbial mats in ultrabasic (pH 10–12) serpentinite springs of the Voltri Massif (Italy). (2) Methods: Along with analysis of chemical and mineralogical parameters, environmental DNA was extracted and subjected to analysis of microbial communities based upon next-generation sequencing. (3) Results: Mineral precipitation and microbialite formation occurred, along with mat formation. Analysis of the serpentinite spring microbial community, based on Illumina sequencing of 16S rRNA amplicons, point to the relevance of alkaliphilic cyanobacteria, colonizing carbonate buildups. Cyanobacterial groups accounted for up to 45% of all retrieved sequences; 3–4 taxa were dominant, belonging to the filamentous groups of Leptolyngbyaceae, Oscillatoriales, and Pseudanabaenaceae. The cyanobacterial community found at these sites is clearly distinct from creek water sediment, highlighting their specific adaptation to these environments.

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“…Metagenomics helps one to understand complicated communities of microorganisms and their working process along with unique ability for identification of new strains and genes [85]. Thermophilic cyanobacterium MTP1 genome is helpful in encoding different resistant system, mainly Cd, Cu, As, Co, Zn, Hg contaminated soils, Which indicates greater potential of this microorganism in remediation of metal contaminated soils [86]. Certain microorganism which are tolerant to contamination for example proteobacteria are tolerant to Cd contamination, possibly can be used to deal with soil Cd contamination [7].…”

Section: Heavy Metal Contamination Diagnosis Through Change In Microbial Community Structure and Diversitymentioning

confidence: 99%

Microbiological Indices for Diagnosis of Heavy Metal Contaminated Soils

Chejara¹,

Kamboj²,

Singh³

et al. 2021

Soil Contamination - Threats and Sustainable Solutions

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Heavy metal contamination has gained popularity worldwide due to their persistent nature in the environment, on the top of that non-biodegradable nature makes its accumulation easy to toxic levels. Understanding the nature of contamination has become a major concern before heavy metals deteriorate the quality of soil; to diagnose heavy metal pollution suitable indices are required. Microbial indices gaining importance because of their sensitive nature towards change in surrounding, which is the imperative quality required to select microbes as environmental indicators. Albeit enough literature is present related to this topic but the information is scattered so role of this chapter is imperative. The chapter will be helpful for the reader to provide a thorough understanding of merits and demerits of microbiological indices for heavy metal contaminated and restituted soils. The changes in microbiological indices and their mechanism of response towards heavy metal stress are effectively summarized. Research gap and future needs of microbial diagnosis of heavy metal contaminated soils are discussed.

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Draft Genome Sequence of a Thermophilic Cyanobacterium from the Family Oscillatoriales (Strain MTP1) from the Chalk River, Colorado

Cited by 6 publications

References 17 publications

Community structure of thermophilic photosynthetic microbial mats and flocs at Sembawang Hot Spring, Singapore

Community structure of thermophilic photosynthetic microbial mats and flocs at Sembawang Hot Spring, Singapore

Cyanobacterial Mats in Calcite-Precipitating Serpentinite-Hosted Alkaline Springs of the Voltri Massif, Italy

Microbiological Indices for Diagnosis of Heavy Metal Contaminated Soils

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