Draft Genome Sequence of a Necrotoxigenic Escherichia coli Isolate

Békal, Sadjia; Vincent, Anne; Berry, Chrystal; Gilmour, Matthew W.; Fournier, Éric; Côté, Jean-Charles; Tremblay, Cécile

doi:10.1128/genomea.01152-15

Cited by 1 publication

(1 citation statement)

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“…NTEC was reported in 4.8% of isolates in our study that is lower than the prevalence 3.49% in diarrheic calves in Iran (Shahrani et al 2014). NTEC is detected in both diarrheic and non-diarrheic animals; thus, there is no clear evidence for its causative role in lamb diarrhea (Bekal et al 2015). Moreover, the combination pattern of cnf2, cdIII, and F17 were found in diarrheic lambs in this study, while this combination pattern was only reported in diarrheic calves previously (Valat et al 2014).…”

Section: Discussioncontrasting

confidence: 69%

Genotypic analysis of virulence genes and antimicrobial profile of diarrheagenic Escherichia coli isolated from diseased lambs in Iran

Ghanbarpour

Askari

Ghorbanpour

et al. 2017

Trop Anim Health Prod

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The aim of the present study was to determine the analysis of virulence genes and antimicrobial profile of diarrheagenic Escherichia coli isolated from diseased lambs. Two hundred ninety E. coli isolates were recovered from 300 rectal swabs of diarrheic lambs and were confirmed by biochemical tests. The pathotype determination was done according to the presence of genes including f5, f41, LTI, STI, bfp, ipaH, stx , stx , eae, ehlyA, cnf , cnf , cdIII, cdIV, and f17 by PCR method. Sixty-six isolates (23.72%) possessed the STI gene and categorized into entrotoxigenic E. coli (ETEC). Nine isolates (3.1%) and five isolates (1.72%) were positive for the cnf1 and cnf2 genes which categorized into necrotoxic E. coli (NTEC). Hundred and seventeen isolates (40.34%) harbored stx and/or stx and classified as Shiga toxin-producing E. coli (STEC). Thirteen isolates (4.48%) were assigned to atypical entropathogenic E. coli (aEPEC) and possessed eae gene. Two isolates (0.68%) were positive for ipaH gene and were assigned to entroinvasive E. coli (EIEC). Statistical analysis showed a specific association between eae gene and STEC pathotype (P < 0.0001). The most prevalent resistance was observed against lincomycin (96.5%) and the lowest resistance was against kanamycine (56.02%), respectively. The high prevalence of STEC and ETEC indicates that diarrheic lambs represent an important reservoir for humans. ETEC may play an important role for frequent occurrence of diarrhea in lambs observed in this region. Due to high antibiotic resistance, appropriate control should be implemented in veterinary medicine to curb the development of novel resistant isolates.

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Section: Discussioncontrasting

confidence: 69%