Draft Genome Sequence of a Polymyxin-Resistant Klebsiella pneumoniae Clinical Strain Carrying
 mcr-8.1
 and
 bla
 NDM-5

Pei, Na; Jian, Zijuan; Liu, Yirui; Liang, Tianzhu; Liu, Wenen; Li, Junhua

doi:10.1128/mra.01224-20

Cited by 4 publications

(3 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The mcr-8 homolog is predominant in our collection, with mcr-1 being the second most common. Unlike the situation in Australia ( 3 ), cocarriage of the carbapenemase gene and mcr was rare in this study, occurring in only one isolate; however, the infected patient was dead in this study ( 15 ).…”

Section: Discussioncontrasting

confidence: 73%

One Health Analysis of mcr -Carrying Plasmids and Emergence of mcr-10.1 in Three Species of Klebsiella Recovered from Humans in China

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Discussioncontrasting

confidence: 73%

One Health Analysis of mcr -Carrying Plasmids and Emergence of mcr-10.1 in Three Species of Klebsiella Recovered from Humans in China

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…Since the mcr-8.1 gene from pig-derived K. pneumoniae strain KP91 was first reported in 2018, multiple mcr-8 variants have been identified, ranging from mcr-8.2 to mcr-8.5 ( Sun et al., 2020 ). Currently, K. pneumoniae producing both MCR-8 and NDM has emerged, such as mcr-8.1 and bla NDM-1 (animal origin), mcr-8.2 and bla NDM-1 (human origin), mcr-8.1 and bla NDM-5 (human origin), and mcr-8.5 and bla NDM-5 (animal origin) ( Wang et al., 2018 ; Ma et al., 2020a ; Sun et al., 2020 ; Pei et al., 2021 ). In our study, we also identified the K. pneumoniae isolate co-harboring mcr-8.2 and bla NDM-5 in the lung transplantation ward, and should be alerted to its in-hospital spread.…”

Section: Discussionmentioning

confidence: 99%

Convergence of MCR-8.2 and Chromosome-Mediated Resistance to Colistin and Tigecycline in an NDM-5-Producing ST656 Klebsiella pneumoniae Isolate From a Lung Transplant Patient in China

Zhao

Zhang

et al. 2022

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

We characterized the first NDM-5 and MCR-8.2 co-harboring ST656 Klebsiella pneumoniae clinical isolate, combining with chromosomal gene-mediated resistance to colistin and tigecycline. The K. pneumoniae KP32558 was isolated from the bronchoalveolar lavage fluid from a lung transplant patient. Complete genome sequences were obtained through Illumina HiSeq sequencing and nanopore sequencing. The acquired resistance genes and mutations in chromosome-encoded genes associated with colistin and tigecycline resistance were analyzed. Comparative genomic analysis was conducted between mcr-8.2-carrying plasmids. The K. pneumoniae KP32558 was identified as a pan-drug resistant bacteria, belonging to ST656, and harbored plasmid-encoded blaNDM-5 and mcr-8.2 genes. The blaNDM-5 gene was located on an IncX3 type plasmid. The mcr-8.2 gene was located on a conjugative plasmid pKP32558-2-mcr8, which had a common ancestor with another two mcr-8.2-carrying plasmids pMCR8_020135 and pMCR8_095845. The MIC of KP32558 for colistin was 256 mg/L. The mcr-8.2 gene and mutations in the two-component system, pmrA and crrB, and the regulator mgrB, had a synergistic effect on the high-level colistin resistance. The truncation in the acrR gene, related to tigecycline resistance, was also identified. K. pneumoniae has evolved a variety of complex resistance mechanisms to the last-resort antimicrobials, close surveillance is urgently needed to monitor the prevalence of this clone.

show abstract

“…Currently, K. pneumoniae producing both MCR-8 and NDM has emerged, such as mcr-8.1 and bla NDM-1 (animal origin), mcr-8.2 and bla NDM-1 (human origin), mcr-8.1 and bla NDM-5 (human origin), and mcr-8.5 and bla NDM-5 (animal origin) (30)(31)(32)(33). In our study, we also identified the K. pneumoniae isolate co-harboring mcr-8.2 and bla NDM-5 in lung transplantation ward, and should be alerted to its in-hospital spread.…”

Section: Discussionmentioning

confidence: 99%

Convergence of MCR-8.2 and chromosome-mediated resistance to colistin and tigecycline in an NDM-5-producing ST656 Klebsiella pneumoniae from a lung transplant patient

Zhao

Zhang

et al. 2021

Preprint

View full text Add to dashboard Cite

For infection caused by NDM-5-producing Klebsiella pneumoniae, tigecycline and colistin are the last treatment options. In this study, we characterized the first NDM-5 and MCR-8.2 co-harboring K. pneumoniae clinical isolate, combining with chromosomal gene-mediated resistance to colistin and tigecycline. The K. pneumoniae KP32558 was isolated from the bronchoalveolar lavage fluid from a lung transplant male patient. Whole genome sequencing was carried out using Illumina HiSeq sequencing platform as well as nanopore sequencing method. The K. pneumoniae KP32558 was identified as a pan-drug resistant bacteria, belonged to ST656, and harbored plasmid-encoded blaNDM-5 and mcr-8.2 genes. The blaNDM-5 gene was located on an IncX3 type plasmid, which was successfully transferred to Escherichia coli strain J53 without visible fitness cost. The mcr-8.2 gene was located on a conjugative plasmid pKP32558-2-mcr8. It had two replicons, IncFII(K) and IncQ1, harbored previously by another two mcr-8.2-carrying plasmids pMCR8_020135 and pMCR8_095845. These three plasmids were clustered into the same clade and derived from K. pneumoniae isolates of the same clonal complex, indicating that pKP32558-2-mcr8 may come from pMCR8_020135 and pMCR8_095845 related ancestor. The MIC of KP32558 for colistin was 256 mg/L, the 6 amino acid substitutions in the two-component system may involve in the high-level colistin resistance. The truncation in acrR gene, related to tigecycline resistance, was also identified. K. pneumoniae has evolved a variety of complex resistance mechanisms to the last-resort antimicrobials, close surveillance is urgently needed to monitor the prevalence of this clone.

show abstract

Draft Genome Sequence of a Polymyxin-Resistant Klebsiella pneumoniae Clinical Strain Carrying mcr-8.1 and bla _NDM-5

Abstract: Carbapenem-resistant Klebsiella pneumoniae (CRKP) is a major threat to global health. Here, we report the draft genome sequence of a Klebsiella pneumoniae clinical strain carrying mcr-8.1 and blaNDM-5.

Cited by 4 publications

References 16 publications

One Health Analysis of mcr -Carrying Plasmids and Emergence of mcr-10.1 in Three Species of Klebsiella Recovered from Humans in China

One Health Analysis of mcr -Carrying Plasmids and Emergence of mcr-10.1 in Three Species of Klebsiella Recovered from Humans in China

Convergence of MCR-8.2 and Chromosome-Mediated Resistance to Colistin and Tigecycline in an NDM-5-Producing ST656 Klebsiella pneumoniae Isolate From a Lung Transplant Patient in China

Convergence of MCR-8.2 and chromosome-mediated resistance to colistin and tigecycline in an NDM-5-producing ST656 Klebsiella pneumoniae from a lung transplant patient

Contact Info

Product

Resources

About

Draft Genome Sequence of a Polymyxin-Resistant Klebsiella pneumoniae Clinical Strain Carrying mcr-8.1 and bla NDM-5

Abstract: Carbapenem-resistant Klebsiella pneumoniae (CRKP) is a major threat to global health. Here, we report the draft genome sequence of a Klebsiella pneumoniae clinical strain carrying mcr-8.1 and blaNDM-5.

Cited by 4 publications

References 16 publications

One Health Analysis of mcr -Carrying Plasmids and Emergence of mcr-10.1 in Three Species of Klebsiella Recovered from Humans in China

One Health Analysis of mcr -Carrying Plasmids and Emergence of mcr-10.1 in Three Species of Klebsiella Recovered from Humans in China

Convergence of MCR-8.2 and Chromosome-Mediated Resistance to Colistin and Tigecycline in an NDM-5-Producing ST656 Klebsiella pneumoniae Isolate From a Lung Transplant Patient in China

Convergence of MCR-8.2 and chromosome-mediated resistance to colistin and tigecycline in an NDM-5-producing ST656 Klebsiella pneumoniae from a lung transplant patient

Contact Info

Product

Resources

About

Draft Genome Sequence of a Polymyxin-Resistant Klebsiella pneumoniae Clinical Strain Carrying mcr-8.1 and bla _NDM-5