Draft Genome Sequence of a Novel
            <i>Serratia</i>
            sp. Strain with Antifungal Activity

Dichmann, Søs I; Park, Byeonghyeok; Pathiraja, Duleepa; Choi, In Geol; Stougaard, Peter; Hennessy, Rosanna C.

doi:10.1128/mra.01340-18

Cited by 3 publications

(5 citation statements)

References 13 publications

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“…During a study aimed at the isolation of bacterial degraders of potato glycoalkaloids, we isolated strain S40 T from a green potato [19]. Based on 16S rRNA gene sequence analysis, the strain showed the highest similarity to Serratia plymuthica PRI-2C [18].…”

Section: Full-textmentioning

confidence: 99%

“…Strain S40 T was isolated in 2018 from potato rhizosphere in Denmark as described by Dichmann et al . [19] and strain PRI-2C, isolated in The Netherlands, 2014, was kindly provided by Prof. P. Garbeva [18]; type strains S. plymuthica LMG 7886 T and Serratia quinivorans LMG 7887 T were from BCCM/LMG, Belgium, and Serratia liquefaciens DSM 4487 T was from DSMZ, Braunschweig, Germany.…”

Section: Isolation Growth and Ecologymentioning

confidence: 99%

“…Complete sequences of the 16S rRNA gene and of the housekeeping genes gyrB , rpoB , infB and atpD were retrieved by blast (https://blast.ncbi.nlm.nih.gov/Blast.cgi) using the S40 T genomic sequence [19] and sequences available in public databases. 16S rRNA gene sequences (1381 bp) were aligned with clustal_w in the CLC Main Workbench, version 8.1.3 (Qiagen) and the alignment was subsequently used for reconstruction of phylogenetic trees in mega 7.0 [21].…”

Section: S Rrna Gene Sequence and Mlsa Analysesmentioning

confidence: 99%

“…Library preparation for whole-genome sequencing was performed using the Nextera DNA library prep kit (Illumina). Sequencing was carried out with an Illumina MiSeq platform using MiSeq reagent kit version 3 (600 cycles) [19]. Digital DNA–DNA hybridization (dDDH) was investigated by comparing genome sequences using the Genome-to-Genome Distance Calculator (GGDC) available at http://ggdc.dsmz.de/distcalc2.php using the alignment method blast + and formula 2 for incomplete genome sequences [23].…”

Section: Genome Featuresmentioning

confidence: 99%

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Serratia inhibens sp. nov., a new antifungal species isolated from potato (Solanum tuberosum)

Hennessy

Dichmann

Martens

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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A novel bacterial strain, S40T, with strong antifungal activity was isolated from the rhizosphere of green potato collected from Zealand, Denmark. Polyphasic analysis with a combined phenotypic, phylogenetic and genomic approach was used to characterize S40T. Phylogenetic analysis based on the 16S rRNA gene and MLSA (concatenated gyrB, rpoD, infB and atpD sequences) showed that strain S40T was affiliated with the genus Serratia and with Serratia plymuthica PRI-2C as the closest related strain [average nucleotide identity (ANI), 99.26 %; DNA–DNA hybridization (dDDH), 99.20%]. However, whole genome sequence analyses revealed that S40T and S. plymuthica PRI-2C genomes displayed lower similarities when compared to all other S. plymuthica strains (ANI ≤94.34 %; dDDH ≤57.6 % relatedness). The DNA G+C content of strain S40T was determined to be 55.9 mol%. Cells of the strain were Gram-negative, rod-shaped, facultative anaerobic and displayed growth at 10–37 °C (optimum, 25–30 °C) and at pH 6–9 (optimum, pH 6–7). Major fatty acids were C16 : 0 (27.9 %), summed feature (C16 : 1 ω6c/C16 : 1 ω7c; 18.0 %) and C17 : 0 cyclo (15.1 %). The respiratory quinone was determined to be Q8 (94 %) and MK8 (95 %) and the major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The results of phenotypic, phylogenetic and genomic analyses support the hypothesis that strain S40T represents a novel species of the genus Serratia , for which the name Serratia inhibens sp. nov. is proposed. The type strain is S40T (=LMG 31467T=NCIMB 15235T). In addition, we propose that S. plymuthica PRI-2C is reclassified and transferred to the species S. inhibens as S. inhibens PRI-2C.

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Section: Full-textmentioning

confidence: 99%

Section: Isolation Growth and Ecologymentioning

confidence: 99%

Section: S Rrna Gene Sequence and Mlsa Analysesmentioning

confidence: 99%

Section: Genome Featuresmentioning

confidence: 99%

See 2 more Smart Citations

Serratia inhibens sp. nov., a new antifungal species isolated from potato (Solanum tuberosum)

Hennessy

Dichmann

Martens

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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“…Genomic DNA was purified from liquid cultures grown at 20°C in lysogenic broth (LB) using the Gentra Puregene Yeast/Bact. kit (Qiagen, Germany) as previously described (3). Library preparation for whole-genome sequencing was performed using the Nextera DNA library prep kit (Illumina, Inc., San Diego, CA).…”

Section: Announcementmentioning

confidence: 99%

Draft Genome Sequences of Two Glycoalkaloid-Degrading Arthrobacter Strains Isolated from Green Potato Peel

Hennessy

Park

Pathiraja

et al. 2019

Microbiol Resour Announc

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Imaging Gene Expression Dynamics in Pseudomonas fluorescens In5 during Interactions with the Fungus Fusarium graminearum PH-1

Hennessy¹,

Stougaard²,

Olsson³

2019

BIO-PROTOCOL

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Genomics, transcriptomics and metabolomics are powerful technologies for studying microbial interactions. The main drawback of these methods is the requirement for destructive sampling. We have established an alternative but complementary technique based on a microplate system combined with promoter fusions for visualizing gene expression in space and time. Here we provide a protocol for measuring spatial and temporal gene expression of a bacterial reporter strain interacting with a fungus on a solid surface.

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Draft Genome Sequence of a Novel Serratia sp. Strain with Antifungal Activity

Abstract: This report describes the draft genome sequence of Serratia sp. strain S40, isolated from potato; it contains 5,383,735 bp and a G+C content of 55.9% and harbors 4,875 predicted coding sequences across 29 contigs.

Cited by 3 publications

References 13 publications

Serratia inhibens sp. nov., a new antifungal species isolated from potato (Solanum tuberosum)

Serratia inhibens sp. nov., a new antifungal species isolated from potato (Solanum tuberosum)

Draft Genome Sequences of Two Glycoalkaloid-Degrading Arthrobacter Strains Isolated from Green Potato Peel

Imaging Gene Expression Dynamics in Pseudomonas fluorescens In5 during Interactions with the Fungus Fusarium graminearum PH-1

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