Draft Genome Sequence of an Isolate of Colletotrichum fructicola, a Causal Agent of Mango Anthracnose

Li, Qili; Bu, Junyan; Yu, Zhihe; Tang, Lihua; Huang, Suiping; Guo, Tangxun; Mo, Jianyou; Hsiang, Tom

doi:10.1128/genomea.00001-18

Cited by 2 publications

(1 citation statement)

References 7 publications

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“…on the economic benefits of fruits, researchers have conducted extensive research on various aspects of its biology, including fungal-plant interaction, genomics and genetics, pathogen infection, cell biology, fungicide resistance, and virulence factors [2,5,6]. At the same Genes 2023, 14, 2216 2 of 18 time, C. fructicola has also been identified as the dominant species causing bitter rot in apple [7], mango anthracnose [8], blueberry anthracnose [9], and strawberry anthracnose [10]. Chemical treatment is the primary method for the field control of anthracnose.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Candidate Reference Genes for Gene Expression Studies Using RT-qPCR in Colletotrichum fructicola from Litchi

Dong,

Huang,

et al. 2023

Genes

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Litchi (Litchi chinensis Sonn.) is a tropical fruit originating from southern China that is currently cultivated in subtropical and tropical regions worldwide. Litchi anthracnose, caused by Colletotrichum fructicola, a dominant species of Colletotrichum spp., is an important disease of litchi that damages the fruits in fields and in post-harvest storage. Real-time quantitative PCR (RT-qPCR) is a common technique with which to detect the expression of and function of target genes quickly and precisely, and stable reference genes are crucial. However, there is no comprehensive information on suitable reference genes of C. fructicola present. Here, we designed eight candidate genes (GAPDH, α-tubulin, 18S, β-tubulin, EF1a, TATA, RPS5, and EF3) using RefFinder software (programs: geNorm, ΔCt, BestKeeper, and NormFinder) to investigate their reliability in the detection of C. fructicola under five different treatments (fungal development stage, temperature, UV, culture medium, and fungicide). The results showed the optimal reference genes under different conditions: EF1a and α-tubulin for developmental stage; α-tubulin and β-tubulin for temperature; α-tubulin and RPS5 for UV treatment; RPS5 and α-tubulin for culture medium; α-tubulin, GAPDH, and TATA for fungicide treatments. The corresponding expression patterns of HSP70 (Heat shock protein 70) were significantly different when the most and the least stable reference genes were selected when treated under different conditions. Our study provides the first detailed list of optimal reference genes for the analysis of gene expression in C. fructicola via RT-qPCR, which should be useful for future functional studies of target genes in C. fructicola.

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Section: Introductionmentioning

confidence: 99%

Assessment of Candidate Reference Genes for Gene Expression Studies Using RT-qPCR in Colletotrichum fructicola from Litchi

Dong,

Huang,

et al. 2023

Genes

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Colletotrichum species associated with anthracnose disease on mango ( Mangifera indica L.) cv. Azúcar in Colombia

Páez-Redondo,

García-Merchán,

Rincón

et al. 2024

Canadian Journal of Plant Pathology

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Draft Genome Sequence of an Isolate of Colletotrichum fructicola, a Causal Agent of Mango Anthracnose

Abstract: Here, we present a draft genome sequence of isolate 15060 of Colletotrichum fructicola, a causal agent of mango anthracnose. The final assembly consists of 1,048 scaffolds totaling 56,493,063 bp (G+C content, 53.38%) and 15,180 predicted genes.

Cited by 2 publications

References 7 publications

Assessment of Candidate Reference Genes for Gene Expression Studies Using RT-qPCR in Colletotrichum fructicola from Litchi

Assessment of Candidate Reference Genes for Gene Expression Studies Using RT-qPCR in Colletotrichum fructicola from Litchi

Colletotrichum species associated with anthracnose disease on mango ( Mangifera indica L.) cv. Azúcar in Colombia

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