Draft Genome Sequence of Bacillus subtilis YBS29, a Potential Fish Probiotic That Prevents Motile Aeromonas Septicemia in Labeo rohita

Abstract

We report the draft genome sequence of the promising fish probiotic Bacillus subtilis YBS29. This strain exhibits in vitro antimicrobial activity against Aeromonas veronii and enhances growth and disease resistance in the Indian major carp species Labeo rohita against motile Aeromonas septicemia (MAS). Its genome contains a gene cluster encoding multiple bacteriocins and lacks genes for virulence factors. These genomic features signify potential for safe use as a probiotic in aquaculture.

Suppression of Streptococcosis and Modulation of the Gut Bacteriome in Nile Tilapia (Oreochromis niloticus) by the Marine Sediment Bacteria Bacillus haynesii and Advenella mimigardefordensis

“…Among beneficial bacteria, Bacillus species are widely used for their beneficial effects on immunity and health and have been reported to be promising probiotics for Nile tilapia ( O. niloticus ) ( 45 , 46 , 48 ). Members of the genus Bacillus are well known for their ability to produce numerous antimicrobial peptides such as the polyketides difficidin, subtilin, subtilosin A, bacillibactin, macrolactin, surfactin, bacilysin, bacillaene, and fengycin, which can protect host organisms through the induction of systemic resistance by inducing the production of stress-related metabolites and the expression of stress response genes ( 49 – 51 ). The potential effects of beneficial Bacillus spp.…”

Friend or Foe: Whole-Genome Sequence of Pseudomonas aeruginosa TG523, Isolated from the Gut of a Healthy Nile Tilapia (Oreochromis niloticus)

“…A paired-end DNA library was prepared using the same DNA used for 16S rRNA sequencing using a Nextera XT library prep kit (Illumina, San Diego, CA) according to the manufacturer’s instructions ( 7 ). Genome sequencing (600 cycles) was carried out using the MiSeq benchtop sequencer (Illumina) ( 8 , 9 ), yielding a total of 5,258,026 reads (2,629,013 paired-end reads) and 1,343,253,926 bases. PRINSEQ v.0.20.3 ( 10 ) was used to assess the quality of the reads.…”