Draft Genome Sequence of Enterococcus durans OSY-EGY, a Multiple-Antimicrobial-Peptide Producer Isolated from Egyptian Hard Cheese

Hussein, Walaa E.; Xiaoli, Lingzi; Yousef, Ahmed E.

doi:10.1128/mra.00303-19

Cited by 3 publications

(5 citation statements)

References 12 publications

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“…A new lactic acid bacterium strain, E. durans OSY-EGY, was isolated from an artisanal cheese and found to have a promising antimicrobial activity that could be beneficial in food applications (Hussein et al, 2019). Before proposing the use of the strain in further industrial applications, research is needed to assess its safety, antimicrobial, and probiotic traits.…”

Section: Resultsmentioning

confidence: 99%

“…Screening E. durans OSY-EGY Genome for Safetyand Probiotic-Related Traits For safety assessment, E. durans OSY-EGY genome (Hussein et al, 2019) was screened using the Comprehensive Antibiotic Resistance Database (CARD) (Jia et al, 2017) and Virulence Factor of Bacterial Pathogens Database (VFDB) 1 (Chen et al, 2005). Additionally, E. durans OSY-EGY genome was mined for genes associated with biosynthetic pathways of biogenic amines using KAAS-KEGG Automatic Annotation Server 2 (Moriya et al, 2007) and KEGG Mapper 3 (Kanehisa et al, 2019).…”

Section: Genotypic Characterizationmentioning

confidence: 99%

“…The rise of antimicrobial resistance in foodborne pathogens and the need for natural food bio-preservatives prompted the search for new strains capable of producing potent antimicrobial agents. E. durans OSY-EGY, used in this study was isolated from artisanal cheese and showed a promising antimicrobial activity against selected pathogenic species (Hussein et al, 2019). The current study was initiated to thoroughly explore the safety, antimicrobial and probiotic traits of E. durans OSY-EGY and its potential usefulness to the industry.…”

Section: Introductionmentioning

confidence: 99%

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Assessment of Safety and Probiotic Traits of Enterococcus durans OSY-EGY, Isolated From Egyptian Artisanal Cheese, Using Comparative Genomics and Phenotypic Analyses

et al. 2020

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An Enterococcus durans strain, designated OSY-EGY, was previously isolated from artisanal cheese. In this work, comparative genomic and phenotypic analyses were utilized to assess the safety characteristics and probiotic traits of the bacterium. The comparative genomic analysis revealed that the strain is distantly related to potentially pathogenic Enterococcus spp. The genome was devoid of genes encoding acquired antibiotic resistance or marker virulence factors associated with Enterococcus spp. Phenotypically, the bacterium is susceptible to vancomycin, ampicillin, tetracycline, chloramphenicol, and aminoglycosides and does not have any hemolytic or gelatinase activity, or cytotoxic effect on Caco-2 cells. Altogether, these findings confirm the lack of hazardous traits in E. durans OSY-EGY. Mining E. durans OSY-EGY genome, for probiotic-related sequences, revealed genes associated with acid and bile salts tolerance, adhesion, competitiveness, antioxidant activitiy, antimicrobial activity, essential amino acids production, and vitamins biosynthesis. Phenotypically, E. durans OSY-EGY was tolerant to acidic pH (3.0), and presence of 0.3% bile salts. The bacterium showed adhesion capability to Caco-2 cells, cholesterol-lowering effect, DPPH scavenging activity, and antimicrobial activity against several Gram-positive pathogenic bacteria. Based on the current work, we propose that E. durans OSY-EGY is a potentially safe strain with desirable probiotic and antimicrobial traits. Thus, the investigated strain could be a promising candidate for several industrial applications.

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Section: Resultsmentioning

confidence: 99%

Section: Genotypic Characterizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Assessment of Safety and Probiotic Traits of Enterococcus durans OSY-EGY, Isolated From Egyptian Artisanal Cheese, Using Comparative Genomics and Phenotypic Analyses

et al. 2020

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“…However, ED26E/7 was hemolysis and gelatinase-negative. The bacteriocin-producing species strain E. durans was also isolated from important Egyptian cheese OSY-W [ 55 ]. Pieniz et al [ 56 ] described E. durans LAB18s isolated from Brazilian soft cheese with the antioxidant activity of its culture supernatant and intracellular extract.…”

Section: Discussionmentioning

confidence: 99%

“…Pieniz et al [ 56 ] described E. durans LAB18s isolated from Brazilian soft cheese with the antioxidant activity of its culture supernatant and intracellular extract. Further beneficial/desirable traits may include the production of antioxidants or the expression of pathogens antagonism [ 55 ]. Dvorožňáková et al [ 57 ] reported the highest stimulative effect on phagocytosis induced by E. durans ED26E/7 strain in mice infected with Trichinella spiralis .…”

Section: Discussionmentioning

confidence: 99%

Slovak Local Ewe’s Milk Lump Cheese, a Source of Beneficial Enterococcus durans Strain

Lauková

Tomáška²,

Kmet

et al. 2021

Foods

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Slovak ewe’s milk lump cheese is produced from unpasteurized ewe’s milk without any added culture. Because of the traditional processing and shaping by hand into a lump, this cheese was given the traditional specialty guaranteed (TSG) label. Up till now, there have existed only limited detailed studies of individual microbiota and their benefits in ewe’s milk lump cheese. Therefore, this study has been focused on the beneficial properties and safety of Enterococcus durans strains with the aim to contribute to basic dairy microbiology but also for further application potential and strategy. The total enterococcal count in cheeses reached 3.93 CFU/g (log 10) ± 1.98 on average. Based on a MALDI-TOF mass spectrometry evaluation, the strains were allotted to the species E. durans (score, 1.781–2.245). The strains were gelatinase and hemolysis-negative (γ-hemolysis) and were mostly susceptible to commercial antibiotics. Among the strains, E. durans ED26E/7 produced the highest value of lactase enzyme β-galactosidase (10 nmoL). ED26E/7 was absent of virulence factor genes such as Hyl (hyaluronidase), IS 16 element and gelatinase (GelE). To test safety, ED26E/7 did not cause mortality in Balb/c mice. Its partially purified bacteriocin substance showed the highest inhibition activity/bioactivity against Gram-positive indicator bacteria: the principal indicator Enterococcus avium EA5 (102,400 AU/mL), Staphylococcus aureus SA5 and listeriae (25,600 AU/mL). Moreover, 16 staphylococci (out of 22) were inhibited (100 AU/mL), and the growth of 36 (out of 51) enterococcal indicators was as well. After further technological tests, E. durans ED26E/7, with its bacteriocin substance, can be supposed as a promising additive to dairy products.

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Applications in food technology: antimicrobial peptides

Huang

Hussein

Campbell

et al. 2021

Biologically Active Peptides

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Draft Genome Sequence of Enterococcus durans OSY-EGY, a Multiple-Antimicrobial-Peptide Producer Isolated from Egyptian Hard Cheese

Cited by 3 publications

References 12 publications

Assessment of Safety and Probiotic Traits of Enterococcus durans OSY-EGY, Isolated From Egyptian Artisanal Cheese, Using Comparative Genomics and Phenotypic Analyses

Assessment of Safety and Probiotic Traits of Enterococcus durans OSY-EGY, Isolated From Egyptian Artisanal Cheese, Using Comparative Genomics and Phenotypic Analyses

Slovak Local Ewe’s Milk Lump Cheese, a Source of Beneficial Enterococcus durans Strain

Applications in food technology: antimicrobial peptides

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