Draft Genome Sequence of Helicobacter suis Strain SNTW101, Isolated from a Japanese Patient with Nodular Gastritis

Matsui, Hidenori; Takahashi, Takuya; Murayama, Somay Yamagata; Uchiyama, Ikuo; Yamaguchi, Katsushi; Shigenobu, Shuji; Suzuki, Masato; Rimbara, Emiko; Shibayama, Keigo; Øverby, Anders; Nakamura, Masahiko

doi:10.1128/genomea.00934-16

Cited by 6 publications

(7 citation statements)

References 11 publications

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“…The first one was made against the homogenate of cultured Hp and reported to react with Helicobacter species, including Hs and Hhss/Ha 13 . The second one was made against the synthetic peptide from the sequence of Hs‐specific outer membrane protein, VacA paralog (HsvA), which is well‐conserved among all the strains of Hs isolated from humans, pigs, and monkeys 14,20,21 . After washing with PBS three times for 5 minutes, we then reacted the specimens with a goat anti‐rabbit IgG conjugated with Alexa Fluor 488, (sb150077, abcam), washed them with PBS three times for 5 minutes, then counterstained with phalloidin‐iFluor reagent (ab176757, abcam) (Alexa Fluor 594) (both of which were diluted 1000 times with PBS) for 30 minutes, and washed with PBS three times for 5 minuutes.…”

Section: Methodsmentioning

confidence: 99%

“…We thus designed the present study to determine the more precise significance of NHPH infection in non‐Hp‐eradicated or post‐Hp‐eradicated human gastric diseases in Japan as revealed by polymerase chain reaction (PCR). In the NHPH‐positive cases, we used polyclonal antibodies against Helicobacters 13 and a Helicobacter suis (Hs) VacA paralog 14 to examine the NHPH immunohistochemically.…”

Section: Introductionmentioning

confidence: 99%

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PCR analysis and specific immunohistochemistry revealing a high prevalence of non‐Helicobacter pylori Helicobacters in Helicobacter pylori‐negative gastric disease patients in Japan: High susceptibility to an Hp eradication regimen

Nakamura

Øverby²,

Michimae

et al. 2020

Helicobacter

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Background The clinical significance of non‐Helicobacter pylori Helicobacter (NHPH) is still unknown. There are many reports of NHPH‐infected patients suffering from gastric diseases. Here, we investigated the polymerase chain reaction (PCR) positivity of NHPH infection in gastric disease patients who were negative for H. pylori (Hp) by the rapid urease test and by pathological observation. Materials and methods We collected the 296 endoscopically obtained gastric mucosal samples of Hp‐negative gastric disease patients diagnosed based on a rapid urease test and pathology from 17 hospitals in Japan from September 2013 to June 2019, and we analyzed the existence of Hp and NHPH by PCR. The samples were also treated by indirect immunohistochemistry using an anti‐Helicobacter suis VacA paralog antibody and were observed by confocal laser microscopy. Results Among the 236 non‐Hp‐eradicated cases, 49 cases (20.8%) were positive for NHPH. Among them, 20 cases were positive for Helicobacter suis, 7 cases were positive for Helicobacter heilmannii sensu stricto/ Helicobacter ailurogastricus (Hhss/Ha), and the other 22 cases could not be identified. The regional differences in the infection rates were significant. Forty percent of the nodular gastritis cases, 24% of the MALT lymphoma, 17% of the chronic gastritis cases, and 33% of the gastroduodenal ulcer cases were NHPH positive. Forty‐five patients had been treated with one of the four types of combinations of a proton pump inhibitor and two antibiotics, and in all of these cases, the NHPH diagnosed by PCR was successfully eradicated. Immunohistochemistry using the Helicobacter suis‐specific HsvA antibody coincided well with the PCR results. Among the 29 post‐Hp eradication cases, three were NHPH positive, including one Hhss/Ha‐positive case. Thus, approx. 20% of the Hp‐negative non‐Hp‐eradicated gastric disease patients treated at 17 hospitals in Japan were infected with NHPH.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

PCR analysis and specific immunohistochemistry revealing a high prevalence of non‐Helicobacter pylori Helicobacters in Helicobacter pylori‐negative gastric disease patients in Japan: High susceptibility to an Hp eradication regimen

Nakamura

Øverby²,

Michimae

et al. 2020

Helicobacter

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“…For immunohistochemistry of H. suis , rabbit polyclonal antibody against H. suis was made. Briefly, a rabbit was immunized with a peptide expected to be antigenic from the vacA paralog reported in a literature 19 . Rabbit IgG was purified from the serum with Protein G affinity column.…”

Section: Methodsmentioning

confidence: 99%

Non-Helicobacter pylori Helicobacter (NHPH) positive gastric cancer

Yasuda

Lee

Nam

et al. 2022

Sci Rep

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Genetic analysis and culturing techniques for gastric non-Helicobacter pylori Helicobacter (NHPH) are progressing. NHPH is reported to accompany nodular gastritis, gastric MALT lymphoma, and mild gastritis. However, only a few gastric cancer cases infected by NHPH have been reported. PCR analysis specific for NHPH and H. pylori was performed for DNA from gastric mucosa of 282 Korean gastric cancer patients, who were treated with endoscopic submucosal dissection. For more precise strain detection of NHPH, NHPH-positive mucosa was stained by immunohistochemistry specific for Helicobacter suis. The Cancer Genome Atlas (TCGA) classification was analyzed for these 3 gastric cancer sub-groups by in situ hybridization and immunohistochemistry. Among 281 patients, 3 patients (1.1%) were positive for NHPH. One patient (Patient 1) was also positive for H. pylori by PCR, another patient (Patient 3) was positive for serum IgG for H. pylori, and the other patient (Patient 2) had no evidence for H. pylori infection. Gastric mucosa of Patients 2 and 3 were positive for H. suis staining. All three NHPH-positive gastric cancers were located in the antrum, and belonged to the Chromosomal Instability Type of TCGA classification. Gastric NHPH can be a cause of gastric cancer, although likely with lower pathogenesis than H. pylori.

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“…We have not successfully cultivated H. suis strains in vitro at this time. Therefore, the H. suis strains TKY (a highly pathogenic strain for mice) and SNTW101 used in this study were isolated from a cynomolgus monkey and a Japanese patient with nodular gastritis, respectively, and individually maintained in the stomachs of C57BL/6 mice (Charles River Japan, Yokohama, Japan) by repeated inoculations of gastric mucosal homogenates from infected to uninfected mice at intervals of around 6 months …”

Section: Methodsmentioning

confidence: 99%

Protective efficacy of a hydroxy fatty acid against gastric Helicobacter infections

et al. 2017

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Draft Genome Sequence of Helicobacter suis Strain SNTW101, Isolated from a Japanese Patient with Nodular Gastritis

Cited by 6 publications

References 11 publications

PCR analysis and specific immunohistochemistry revealing a high prevalence of non‐Helicobacter pylori Helicobacters in Helicobacter pylori‐negative gastric disease patients in Japan: High susceptibility to an Hp eradication regimen

PCR analysis and specific immunohistochemistry revealing a high prevalence of non‐Helicobacter pylori Helicobacters in Helicobacter pylori‐negative gastric disease patients in Japan: High susceptibility to an Hp eradication regimen

Non-Helicobacter pylori Helicobacter (NHPH) positive gastric cancer

Protective efficacy of a hydroxy fatty acid against gastric Helicobacter infections

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