Draft Genome Sequence of
            <i>Streptomyces</i>
            sp. Strain Wb2n-11, a Desert Isolate with Broad-Spectrum Antagonism against Soilborne Phytopathogens

Köberl, Martina; White, Richard Allen; Erschen, Sabine; El-Arabi, Tarek F.; Jansson, Janet; Berg, Gabriele

doi:10.1128/genomea.00860-15

Cited by 16 publications

(10 citation statements)

References 11 publications

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“…Rhizobium (Patel and Sinha, 2011 (Köberl et al, 2015), and have many mobile elements, which make them difficult to engineer and/or grow in cooperative synthetic microbial communities. The base of a synthetic community would be Bacillus spp., as it is relatively easy to genetically engineer (Dong and Zhang, 2014), has a large depth of complete genome sequences (Sharma and Satyanarayana, 2013), contains many isolates that have plant growth proprieties (Köberl et al, 2013;Köberl et al, 2015), and/or are currently used in bio-control applications.…”

Section: Rhizosphere Engineering Via Synthetic Rhizospheric Microbialmentioning

confidence: 99%

“…The base of a synthetic community would be Bacillus spp., as it is relatively easy to genetically engineer (Dong and Zhang, 2014), has a large depth of complete genome sequences (Sharma and Satyanarayana, 2013), contains many isolates that have plant growth proprieties (Köberl et al, 2013;Köberl et al, 2015), and/or are currently used in bio-control applications. For example, a Bacillus spp.…”

Section: Rhizosphere Engineering Via Synthetic Rhizospheric Microbialmentioning

confidence: 99%

See 1 more Smart Citation

Rhizosphere engineering: Enhancing sustainable plant ecosystem productivity

White²,

et al. 2017

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The rhizosphere is arguably the most complex microbial habitat on earth, comprising an integrated network of plant roots, soil and a diverse microbial consortium of bacteria, archaea, viruses, and microeukaryotes. Understanding, predicting and controlling the structure and function of the rhizosphere will allow us to harness plantmicrobe interactions and other rhizosphere activities as a means to increase or restore plant ecosystem productivity, improve plant responses to a wide range of environmental perturbations, and mitigate effects of climate change by designing ecosystems for longterm soil carbon storage. Here, we review critical knowledge gaps in rhizosphere 2 science, and how mechanistic understanding of rhizosphere interactions can be leveraged in rhizosphere engineering efforts with the goal of maintaining sustainable plant ecosystem services for food and bioenergy production in an ever changing global climate.

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Section: Rhizosphere Engineering Via Synthetic Rhizospheric Microbialmentioning

confidence: 99%

Section: Rhizosphere Engineering Via Synthetic Rhizospheric Microbialmentioning

confidence: 99%

Rhizosphere engineering: Enhancing sustainable plant ecosystem productivity

White²,

et al. 2017

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“…This (8.2 Mb) larger genome size is closer to that (8.6 Mb) of model strain Streptomyces coelicolor A3 [ 36 ] and equal to the genome of Streptomyces sp. Wb2n-1132—native to a desert environment [ 37 ]. Sequence data were deposited in the GenBank under accession number NISY00000000.…”

Section: Resultsmentioning

confidence: 99%

“…Similarly, genome of desert isolate Streptomyces sp. Wb2n-11 has been reported to have 3368 hypothetical proteins [ 37 ].…”

Section: Resultsmentioning

confidence: 99%

Desert Environments Facilitate Unique Evolution of Biosynthetic Potential in Streptomyces

et al. 2021

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Searching for new bioactive metabolites from the bacterial genus Streptomyces is a challenging task. Combined genomic tools and metabolomic screening of Streptomyces spp. native to extreme environments could be a promising strategy to discover novel compounds. While Streptomyces of desertic origin have been proposed as a source of new metabolites, their genome mining, phylogenetic analysis, and metabolite profiles to date are scarcely documented. Here, we hypothesized that Streptomyces species of desert environments have evolved with unique biosynthetic potential. To test this, along with an extensive characterization of biosynthetic potential of a desert isolate Streptomyces sp. SAJ15, we profiled phylogenetic relationships among the closest and previously reported Streptomyces of desert origin. Results revealed that Streptomyces strains of desert origin are closer to each other and relatively distinct from Streptomyces of other environments. The draft genome of strain SAJ15 was 8.2 Mb in size, which had 6972 predicted genes including 3097 genes encoding hypothetical proteins. Successive genome mining and phylogenetic analysis revealed the presence of putative novel biosynthetic gene clusters (BGCs) with low incidence in another Streptomyces. In addition, high-resolution metabolite profiling indicated the production of arylpolyene, terpenoid, and macrolide compounds in an optimized medium by strain SAJ15. The relative abundance of different BGCs in arid Streptomyces differed from the non-arid counterparts. Collectively, the results suggested a distinct evolution of desert Streptomyces with a unique biosynthetic potential.

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“…DH-12 (Thar desert, India) [15], and Streptomyces sp. Wb2n-11 (Sinai desert, Egypt) [16] and were also included in the present study. These taxonomical relatives of JB150 belonging to the different desert ecosystems were purposefully selected to investigate the genetic assortment and similarities that confer upon them the ability to sustain and thrive under the harsh conditions of deserts and produce a huge repertoire of diverse secondary metabolites.…”

Section: Streptomyces Have Linear Chromosomes and Their Genome Size Rmentioning

confidence: 99%

Comparative whole-genome analysis of a Thar desert strainStreptomycessp. JB150 provides deep insights into the encoded parvome and adaptations to desert edaphic system

Harwani

Begani

Lakhani

2021

Preprint

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We sequenced the genome of Streptomyces sp. JB150, isolated from a unique site of the Thar desert in India. Genome mining of the JB150 genome revealed the presence of many interesting secondary metabolic biosynthetic gene clusters (BGCs). The encoded parvome of JB150 includes non-ribosomal peptides, polyketides including β-lactone, butyrolactone, ectoine, lantipeptides, lasso peptides, melanin, resorcinol, siderophores, terpenoids, thiopeptides, and other types of hybrid compounds. Among them, ~30% BGCs displayed a high degree of novelty. The genome of JB150 was enriched for a large assortment of specialized genes coding for the production of many interesting biomolecules comprising compatible solutes, multiple stress-response regulators, transport proteins, protein secretion systems, signaling molecules, chaperones and storage reserves, etc. The presence of diverse members of CAZymes enzyme families, high numbers of riboflavin, flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), trehalose and aromatic compounds synthesis genes, putative orthologues to several of the classical fatty acid synthesis components, prototrophy for many essential amino acids exhibit metabolic versatility of JB150 to inhabit in the extreme desert environment. Besides, the genome of JB150 was observed to specifically encode thiazole-oxazole-modified thiazolemicrocin (TOMM) and ectoine. The comparison of the complete genomes of Streptomyces sp. JB150 and seven other actinomycete strains belonging to different desert ecosystems unveiled the presence of many previously undetected, distinctive, biological, and genomic signatures. We propose that these genetic traits endowed by these strains are essential for their adaptation in the highly underprivileged, extreme ecosystem of the Thar desert to cope with multiple abiotic stressors, oligotrophic nutrient conditions and to produce a huge repertoire of diverse secondary metabolites.

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Draft Genome Sequence of Streptomyces sp. Strain Wb2n-11, a Desert Isolate with Broad-Spectrum Antagonism against Soilborne Phytopathogens

Cited by 16 publications

References 11 publications

Rhizosphere engineering: Enhancing sustainable plant ecosystem productivity

Rhizosphere engineering: Enhancing sustainable plant ecosystem productivity

Desert Environments Facilitate Unique Evolution of Biosynthetic Potential in Streptomyces

Comparative whole-genome analysis of a Thar desert strainStreptomycessp. JB150 provides deep insights into the encoded parvome and adaptations to desert edaphic system

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