Draft genome sequence of Lactobacillus plantarum strain DMR17 isolated from homemade cow dahi of Sikkim Himalayan region: an evaluation of lactate fermentation and secondary metabolism

Tirwa, Ranjan Kaushal; Najar, Ishfaq Nabi; Thakur, Nagendra; Chaurasia, Lalit K.; Tamang, Buddhiman

doi:10.1007/s00203-020-02023-6

Cited by 3 publications

(2 citation statements)

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“…In comparison with LAB isolated from quinoa-based fermented yogurt (Ujiroghene et al, 2019 ) and Lacticaseibacillus casei (RAMULAB07 and RAMULAB08) and Limosilactobacillus fermentum (RAMULAB09, RAMULAB10, RAMULAB11, and RAMULAB12) isolated from dosa batter (Kumari et al, 2022 ). In addition, the isolate used in the present study showed a higher ability to inhibit α-amylase compared to L. plantarum DMR15 (Tirwa et al, 2020 ) and camel milks fermented by L. plantarum DSM2468, L. reuteri -KX88apoprotein1777, and L. plantarum -KX881779 (Ayyash et al, 2018 ). In this study CFS exhibited the best inhibiition against α-glucosidase and α-amylase (carbohydrate hydrolyzing enzymes) in comparison to IC and CFE.…”

Section: Discussionmentioning

confidence: 68%

Screening for potential novel probiotic Levilactobacillus brevis RAMULAB52 with antihyperglycemic property from fermented Carica papaya L.

Sreepathi,

Kumari,

Huligere

et al. 2023

Front. Microbiol.

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Probiotics are live microorganisms with various health benefits when consumed in appropriate amounts. Fermented foods are a rich source of these beneficial organisms. This study aimed to investigate the probiotic potential of lactic acid bacteria (LAB) isolated from fermented papaya (Carica papaya L.) through in vitro methods. The LAB strains were thoroughly characterized, considering their morphological, physiological, fermentative, biochemical, and molecular properties. The LAB strain's adherence and resistance to gastrointestinal conditions, as well as its antibacterial and antioxidant capabilities, were examined. Moreover, the strains were tested for susceptibility against specific antibiotics, and safety evaluations encompassed the hemolytic assay and DNase activity. The supernatant of the LAB isolate underwent organic acid profiling (LCMS). The primary objective of this study was to assess the inhibitory activity of α-amylase and α-glucosidase enzymes, both in vitro and in silico. Gram-positive strains that were catalase-negative and carbohydrate fermenting were selected for further analysis. The LAB isolate exhibited resistance to acid bile (0.3% and 1%), phenol (0.1% and 0.4%), and simulated gastrointestinal juice (pH 3–8). It demonstrated potent antibacterial and antioxidant abilities and resistance to kanamycin, vancomycin, and methicillin. The LAB strain showed autoaggregation (83%) and adhesion to chicken crop epithelial cells, buccal epithelial cells, and HT-29 cells. Safety assessments indicated no evidence of hemolysis or DNA degradation, confirming the safety of the LAB isolates. The isolate's identity was confirmed using the 16S rRNA sequence. The LAB strain Levilactobacillus brevis RAMULAB52, derived from fermented papaya, exhibited promising probiotic properties. Moreover, the isolate demonstrated significant inhibition of α-amylase (86.97%) and α-glucosidase (75.87%) enzymes. In silico studies uncovered that hydroxycitric acid, one of the organic acids derived from the isolate, interacted with crucial amino acid residues of the target enzymes. Specifically, hydroxycitric acid formed hydrogen bonds with key amino acid residues, such as GLU233 and ASP197 in α-amylase, and ASN241, ARG312, GLU304, SER308, HIS279, PRO309, and PHE311 in α-glucosidase. In conclusion, Levilactobacillus brevis RAMULAB52, isolated from fermented papaya, possesses promising probiotic properties and exhibits potential as an effective remedy for diabetes. Its resistance to gastrointestinal conditions, antibacterial and antioxidant abilities, adhesion to different cell types, and significant inhibition of target enzymes make it a valuable candidate for further research and potential application in the field of probiotics and diabetes management.

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