Draft Genome Sequence of Ligilactobacillus salivarius FFIG58, Isolated from the Intestinal Tract of Wakame-Fed Pig

Zhou, Bingwen; Albarracín, Leonardo; Masumizu, Yuki; Indo, Yuhki; Islam, M. S.; García-Castillo, Valeria; Ikeda-Ohtsubo, Wakako; Suda, Yoshihito; Aso, Hisashi; Villena, Julio; Kitazawa, Haruki

doi:10.1128/mra.00839-20

Cited by 1 publication

(4 citation statements)

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“…The complete genome sequence of L. salivarius FFIG58 was reported recently [23]. Seven additional FFIG strains were selected for complete genome sequencing in this work.…”

Section: Complete Genome Sequencingmentioning

confidence: 99%

“…Seven additional FFIG strains were selected for complete genome sequencing in this work. Single colonies of the selected L. salivarius FFIG strains cultured on MRS (Oxoid, Cambridge, UK) agar plates were inoculated separately into MRS broth and incubated at 37 • C for 12 h. The isolation of the genomic DNA was performed by using a lysozyme lysis buffer (75 mM NaCl, 20 mM EDTA, 20 mM Tris-HCl, pH 7.5, 10 mg/mL lysozyme), the chloroform-isoamyl alcohol separation, and isopropanol precipitation method as described previously [23,24]. The genomic DNA samples were sequenced with an Illumina HiSeq platform using the 2 × 300-bp paired-end read length-sequencing protocol.…”

Section: Complete Genome Sequencingmentioning

confidence: 99%

“…The paired-end sequencing libraries were prepared using the TruSeq DNA HT Sample Prep Kit (Illumina) according to the manufacturer's protocol. The paired-end reads were filtered with PrinSeq-lite (v.0.20.4) and assembled with A5-miseq (v.201690825) with default parameters [23].…”

Section: Complete Genome Sequencingmentioning

confidence: 99%

“…To deepen the characterization of the selected L. salivarius strains, their complete genomes were sequenced by Illumina HiSeq. The genome of L. salivarius FFIG58 was annotated and published recently [23], while the genomes of FFIG23, FFIG53, FFIG60, FFIG63, FFIG79, FFIG124, and FFIG130 were sequenced for this work (Table 1). The FFIG58 draft genome sequence has an average GC content of 32.9% and a total estimated size of 1,984,180 bp (Table 1).…”

Section: General Genomic Features Of L Salivarius Isolated From the Intestinal Tract Of Wakame-fed Pigsmentioning

confidence: 99%

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Selection of Immunobiotic Ligilactobacillus salivarius Strains from the Intestinal Tract of Wakame-Fed Pigs: Functional and Genomic Studies

et al. 2020

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In this article, Ligilactobacillus salivarius FFIG strains, isolated from the intestinal tract of wakame-fed pigs, are characterized according to their potential probiotic properties. Strains were evaluated by studying their interaction with porcine intestinal epithelial (PIE) cells in terms of their ability to regulate toll-like receptor (TLR)-3- or TLR4-mediated innate immune responses, as well as by assessing their adhesion capabilities to porcine epithelial cells and mucins. These functional studies were complemented with comparative genomic evaluations using the complete genome sequences of porcine L. salivarius strains selected from subgroups that demonstrated different “immune” and “adhesion” phenotypes. We found that their immunomodulatory and adhesion capabilities are a strain-dependent characteristic. Our analysis indicated that the differential immunomodulatory and adhesive activities of FFIG strains would be dependent on the combination of several surface structures acting simultaneously, which include peptidoglycan, exopolysaccharides, lipoteichoic acid, and adhesins. Of note, our results indicate that there is no correlation between the immunomodulatory capacity of the strains with their adhesion ability to mucins and epithelial cells. Therefore, in the selection of strains destined to colonize the intestinal mucosa and modulate the immunity of the host, both properties must be adequately evaluated. Interestingly, we showed that L. salivarius FFIG58 functionally modulated the innate immune responses triggered by TLR3 and TLR4 activation in PIE cells and efficiently adhered to these cells. Moreover, the FFIG58 strain was capable of reducing rotavirus replication in PIE cells. Therefore, L. salivarius FFIG58 is a good candidate for further in vivo studying the protective effect of lactobacilli against intestinal infections in the porcine host. We also reported and analyzed, for the first time, the complete genome of several L. salivarius strains that were isolated from the intestine of pigs after the selective pressure of feeding the animals with wakame. Further genomic analysis could be of value to reveal the metabolic characteristics and potential of the FFIG strains in general and of the FFIG58 strain, in particular, relating to wakame by-products assimilation.

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“…The complete genome sequence of L. salivarius FFIG58 was reported recently [23]. Seven additional FFIG strains were selected for complete genome sequencing in this work.…”

Section: Complete Genome Sequencingmentioning

confidence: 99%

Section: Complete Genome Sequencingmentioning

confidence: 99%

Section: Complete Genome Sequencingmentioning

confidence: 99%

Section: General Genomic Features Of L Salivarius Isolated From the Intestinal Tract Of Wakame-fed Pigsmentioning

confidence: 99%

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