Draft Genome Sequence of Petroleum Hydrocarbon-Degrading Pseudomonas aeruginosa Strain PK6, Isolated from the Saurashtra Region of Gujarat, India

Patel, P. A.; Kothari, Vishal; Kothari, Charmy; Faldu, Priti; Domadia, K. K.; Rawal, C. M.; Bhimani, H. D.; Parmar, Nidhi; Nathani, Neelam M.; Koringa, Prakash G.; Joshi, Chaitanya G.; Kothari, Ramesh

doi:10.1128/genomea.00002-14

Cited by 8 publications

(3 citation statements)

References 7 publications

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“…Pseudomonas aeruginosa is a gamma proteobacterium which is known to produce rhamnolipid and able to utilize and grow in high concentrations of crude oil. Rhamnolipid itself has been reported capable of stimulating biodegradation of hydrocarbons (Patel et al 2014).Udochukwu et al (2014 stated that P. aeroginosa is the predominant species in petroleum degradation and always found in every oil pollution site analyzed. Chamkha et al (2011) successfully isolated Klebsiella oxytoca strain BSC5 from an off shore oil field in Tunisia.…”

Section: Resultsmentioning

confidence: 99%

IDENTIFICATION OF POTENTIAL DIESEL OIL-DEGRADING BACTERIA ISOLATED FROM MANADO SEA PORT BASED ON 16S rRNA GENE

Abram¹,

Tallei²,

Queljoe³

et al. 2014

JIS

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Petroleum contamination and its derivate in ecosystem are considered as environmental threat all over the world. Some microorganisms exhibit potential to degrade hydrocarbon in contaminated environments. This study aims at identifying potential diesel oil-degrading bacteria grown on artificial media. Bacteria isolated from Manado Sea port were grown in nutrient agar containing artificial diesel oil plus salt water and diesel oil only, respectively. The growing bacteria were isolated and each of them was grown separately to obtain pure isolate. Three bacterial isolates namely AO2, OA3 and OA4 were identified using 16S rRNA gene as Pseudomonas aeroginosa, Klebsiella oxytoca, and Citrobacter sp, respectively. Keywords: diesel oil, diesel oil-degrading bacteria, Manado Sea Port, 16S rRNA gene IDENTIFIKASI BAKTERI YANG BERPOTENSI SEBAGAI PENDEGRADASI MINYAK DIESEL DI ISOLASI DARI PELABUHAN LAUT MANADO ABSTRAK Kontaminasi minyak bumi dan turunannya dalam ekosistem dianggap sebagai ancaman lingkungan di seluruh dunia. Beberapa mikroorganisme menunjukkan potensi yang dapat menurunkan hidrokarbon dalam lingkungan yang terkontaminasi. Penelitian ini bertujuan untuk mengidentifikasi bakteri yang berpotensi sebagai pendegradasi minyak yang tumbuh pada media buatan. Bakteri diisolasi dari pelabuhan laut Manado dan ditumbuhkan dalam media NA yang mengandung minyak diesel dengan penambahan air garam buatan dan minyak diesel tanpa air garam buatan. Bakteri yang tumbuh diisolasi dan masing-masing ditanam secara terpisah untuk mendapatkan isolat murni. Tiga isolat bakteri yaitu AO2, AO3 dan AO4 yang telah diidentifikasi menggunakan 16S rRNA gen secara berturut-turut adalah Pseudomonas aeroginosa, Klebsiella oxytoca, dan Citrobacter sp. Kata kunci: minyak diesel, bakteri pendegradasi minyak diesel, Pelabuhan Laut Manado, gen 16S rRNA

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Section: Resultsmentioning

confidence: 99%

IDENTIFICATION OF POTENTIAL DIESEL OIL-DEGRADING BACTERIA ISOLATED FROM MANADO SEA PORT BASED ON 16S rRNA GENE

Abram¹,

Tallei²,

Queljoe³

et al. 2014

JIS

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“…If the pesticide is not degraded or detoxified quickly enough, the risk of its off-site migration may pose health risk to humans (Fulekar and Geetha, 2008). The manufacturing and formulation process of chlorpyrifos also generate waste which needs to be treated (Patel et al 2014). Catabolism and detoxification metabolism occur when a soil microorganism uses the pesticide as a carbon and energy source.…”

Section: Introductionmentioning

confidence: 99%

Isolation, characterization and growth response study of chlorpyrifos utilizing soil bacterium Pseudomonas putida JR16

Rank

Nathani

Hinsu

et al. 2018

IJARe

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Agricultural pesticides are one of the indispensable materials used to meet increasing demand of food crops. Consequently, pesticides find their way to various ecosystems resulting in detrimental pollution problems. Chlorpyrifos is a predominantly used organophosphorus pesticide leading to harmful effects including abnormal cell division. Scientific attempt to eliminate these contaminants from the environment using biodegradation approach has been appreciated. Soil bacteria capable of utilizing chlorpyrifos are potential bioremediation candidates. In context to the same, the present study isolated six bacteria growing in presence of chlorpyrifos as sole carbon source from agricultural soils. The obtained bacterial isolates characterized based on their morphological characteristics, biochemical reactions were found to be Pseudomonas spp. JR16, Pseudomonas spp. J1, Pseudomonas spp. J2, Pantoea spp. J3, Enterobacter spp. J4 and Kocuria spp. J5. Among these, the most potential of them viz., Pseudomonas spp. JR16, was further proceeded for molecular identification and was observed to be the Pseudomonas putida JR16. The influence of different chlorpyrifos concentration, various carbon sources, metals sources, temperature and pH on the growth of Pseudomonas putida JR16 was assessed for developing optimum conditions for on field application of the strain for biodegradation of chlorpyrifos.

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“…It have been reported that Pseudomonas sp possesses genes that code for enzymes such as catechol dioxygenase, alkane 1-monooxygenase and alkane sulfonate monooxygenase [16] that help in the breaking down of hydrocarbon chains.…”

Section: Determination Of Soil Percentage Oxidizible Organic Carbon mentioning

confidence: 99%

Variation in the Carbon (C), Phosphorus (P) and Nitrogen (N) Utilization during the Biodegradation of Crude Oil in Soil

A¹,

S²,

Ine³

2015

J Pet Environ Biotechnol

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This study was aimed at determining the effect of varying concentration of crude oil pollution on the macronutrients in the soil. Various macro-nutrients (such as soil ammonium concentration, soil nitrate concentration, and available phosphorus), oxidizable organic carbon, and total petroleum hydrocarbon were determined. The result revealed that as the time increases, the oxidizable carbon, which is also a function of the organic matter decreases, which is as a result of the conversion of carbon to carbon (IV) oxide during cellular metabolism. This decrease showed that there was an increase in the activity of that leads to the breakdown of the carbon components in the soil. The soil phosphate concentration determination did not show any pattern in their increase or decrease, which shows that increase in crude oil concentration, did not significantly affect the phosphate concentration in the soil. The soil ammonium concentration increased from 24 th hour to 168 th hour but decreased before the end of the experiment. This increase could be attributed to ability of the Azotobacter vinelandii to fixed nitrogen as an innate responsibility, while Pseudomonas sp. which is known to contain nitrogen fixing genes. The result also showed that there is a constant increase in soil nitrate concentration which is affected as the concentration of the pollutant (crude oil) increases. This study has shown that the consortium of these organisms can be used as a bio-fertilizer as well as in bioremediation.

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Draft Genome Sequence of Petroleum Hydrocarbon-Degrading Pseudomonas aeruginosa Strain PK6, Isolated from the Saurashtra Region of Gujarat, India

Cited by 8 publications

References 7 publications

IDENTIFICATION OF POTENTIAL DIESEL OIL-DEGRADING BACTERIA ISOLATED FROM MANADO SEA PORT BASED ON 16S rRNA GENE

IDENTIFICATION OF POTENTIAL DIESEL OIL-DEGRADING BACTERIA ISOLATED FROM MANADO SEA PORT BASED ON 16S rRNA GENE

Isolation, characterization and growth response study of chlorpyrifos utilizing soil bacterium Pseudomonas putida JR16

Variation in the Carbon (C), Phosphorus (P) and Nitrogen (N) Utilization during the Biodegradation of Crude Oil in Soil

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