Draft Genome Sequence of Pseudomonas syringae pv. syringae ALF3 Isolated from Alfalfa

Harrison, James W.; Dornbusch, Melinda R.; Samac, Deborah A.; Studholme, David J.

doi:10.1128/genomea.01722-15

Cited by 7 publications

(5 citation statements)

References 11 publications

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“…From a glycerol stock, the sequenced bacterial strain, Pseudomonas syringae pv. syringae ALF3, originally isolated from an infected alfalfa plant near Cheyenne, WY, was cultured on nutrient broth yeast extract (NBY) agar at 30 °C [28]. ALF3 was used as the wild type strain of P. syringae pv.…”

Section: Bacterial Strains and Growth Mediamentioning

confidence: 99%

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Plant defensin antibacterial mode of action against Pseudomonas species

Sathoff

Lewenza

Samac

2020

Preprint

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Background: Though many plant defensins exhibit antibacterial activity, little is known about their antibacterial mode of action (MOA). Antimicrobial peptides with a characterized MOA induce the expression of multiple bacterial outer membrane modifications, which are required for resistance to these membrane-targeting peptides. Mini-Tn5-lux mutant strains of Pseudomonas aeruginosa with Tn insertions disrupting outer membrane protective modifications were assessed for sensitivity against plant defensin peptides. These transcriptional lux reporter strains were also evaluated for lux gene expression in response to sublethal plant defensin exposure. Also, a plant pathogen, Pseudomonas syringae pv. syringae was modified through transposon mutagenesis to create mutants that are resistant to in vitro MtDef4 treatments.Results: Plant defensins displayed specific and potent antibacterial activity against strains of P. aeruginosa. A defensin from Medicago truncatula, MtDef4, induced dose-dependent gene expression of the aminoarabinose modification of LPS and surface polycation spermidine production operons. The ability for MtDef4 to damage bacterial outer membranes was also verified visually through fluorescent microscopy. Another defensin from M. truncatula, MtDef5, failed to induce lux gene expression and limited outer membrane damage was detected with fluorescent microscopy. The transposon insertion site on MtDef4 resistant P. syringae pv. syringae mutants was sequenced, and modifications of ribosomal genes were identified to contribute to enhanced resistance to plant defensin treatments. Conclusions: MtDef4 damages the outer membrane similar to polymyxin B, which stimulates antimicrobial peptide resistance mechanisms to plant defensins. MtDef5, appears to have a different antibacterial MOA. Additionally, the MtDef4 antibacterial mode of action may also involve inhibition of translation.

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Section: Bacterial Strains and Growth Mediamentioning

confidence: 99%

“…P. syringae pv. syringae strain ALF3, pathogenic on alfalfa and M. truncatula, has a draft genome sequence [28] and was shown to be sensitive to M. truncatula defensins, MtDef5 and MtDef4, with IC 50 values of 0.1 and 0.4 µM, respectively [3]. Additionally, MtDef4 displays activity against Xanthomonas alfalfae subsp.…”

Section: Introductionmentioning

confidence: 99%

Plant defensin antibacterial mode of action against Pseudomonas species

Sathoff

Lewenza

Samac

2020

Preprint

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“…In the initial stage, the bacterial penetration forms water-soaked lesions at the frost injury sites, which further extends and produces dried bacterial exudate throughout the stem. The diseased plants are usually dwarfs, with slender stems that blacken upon maturity [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

“…syringae on the basis of the 16S rDNA sequence and PCR amplification of syrB for toxin production [ 11 ]. Following the genome sequencing of the strain ALF3 [ 8 ], it was found to be pathogenic in various plants such as Medicago truncatula , pear leaves, and bean seed pods [ 11 ]. Significant differentially expressed genes and processes associated with the host defense mechanism have also been proposed in alfalfa infected with bacterial stem blight [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

alfaNET: A Database of Alfalfa-Bacterial Stem Blight Protein–Protein Interactions Revealing the Molecular Features of the Disease-causing Bacteria

Kataria

Kaundal

2021

IJMS

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Alfalfa has emerged as one of the most important forage crops, owing to its wide adaptation and high biomass production worldwide. In the last decade, the emergence of bacterial stem blight (caused by Pseudomonas syringae pv. syringae ALF3) in alfalfa has caused around 50% yield losses in the United States. Studies are being conducted to decipher the roles of the key genes and pathways regulating the disease, but due to the sparse knowledge about the infection mechanisms of Pseudomonas, the development of resistant cultivars is hampered. The database alfaNET is an attempt to assist researchers by providing comprehensive Pseudomonas proteome annotations, as well as a host–pathogen interactome tool, which predicts the interactions between host and pathogen based on orthology. alfaNET is a user-friendly and efficient tool and includes other features such as subcellular localization annotations of pathogen proteins, gene ontology (GO) annotations, network visualization, and effector protein prediction. Users can also browse and search the database using particular keywords or proteins with a specific length. Additionally, the BLAST search tool enables the user to perform a homology sequence search against the alfalfa and Pseudomonas proteomes. With the successful implementation of these attributes, alfaNET will be a beneficial resource to the research community engaged in implementing molecular strategies to mitigate the disease. alfaNET is freely available for public use at http://bioinfo.usu.edu/alfanet/.

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“…syringae pv. syringae (PssALF3) and a complete genome sequence was obtained [ 9 ]. It was found to be pathogenic on alfalfa, Medicago truncatula (barrel medic), bean seedpods, pear leaves, and beet seedlings but did not cause disease on bean leaves [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Resistant and susceptible responses in alfalfa (Medicago sativa) to bacterial stem blight caused by Pseudomonas syringae pv. syringae

et al. 2017

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Bacterial stem blight caused by Pseudomonas syringae pv. syringae is a common disease of alfalfa (Medicago sativa L). Little is known about host-pathogen interactions and host defense mechanisms. Here, individual resistant and susceptible plants were selected from cultivars Maverick and ZG9830 and used for transcript profiling at 24 and 72 hours after inoculation (hai) with the isolate PssALF3. Bioinformatic analysis revealed a number of differentially expressed genes (DEGs) in resistant and susceptible genotypes. Although resistant plants from each cultivar produced a hypersensitive response, transcriptome analyses indicated that they respond differently at the molecular level. The number of DEGs was higher in resistant plants of ZG9830 at 24 hai than in Maverick, suggesting that ZG9830 plants had a more rapid effector triggered immune response. Unique up-regulated genes in resistant ZG9830 plants included genes encoding putative nematode resistance HSPRO2-like proteins, orthologs for the rice Xa21 and soybean Rpg1-b resistance genes, and TIR-containing R genes lacking both NBS and LRR domains. The suite of R genes up-regulated in resistant Maverick plants had an over-representation of R genes in the CC-NBS-LRR family including two genes for atypical CCR domains and a putative ortholog of the Arabidopsis RPM1 gene. Resistance in both cultivars appears to be mediated primarily by WRKY family transcription factors and expression of genes involved in protein phosphorylation, regulation of transcription, defense response including synthesis of isoflavonoids, and oxidation-reduction processes. These results will further the identification of mechanisms involved in resistance to facilitate selection of parent populations and development of commercial varieties.

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Draft Genome Sequence of Pseudomonas syringae pv. syringae ALF3 Isolated from Alfalfa

Cited by 7 publications

References 11 publications

Plant defensin antibacterial mode of action against Pseudomonas species

Plant defensin antibacterial mode of action against Pseudomonas species

alfaNET: A Database of Alfalfa-Bacterial Stem Blight Protein–Protein Interactions Revealing the Molecular Features of the Disease-causing Bacteria

Resistant and susceptible responses in alfalfa (Medicago sativa) to bacterial stem blight caused by Pseudomonas syringae pv. syringae

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