2022
DOI: 10.1128/mra.00236-22
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Draft Genome Sequence of Serratia marcescens SSA1, Isolated from Dioxin-Polluted Soil

Abstract: Serratia marcescens SSA1 was isolated from a dump site with a history of incineration. Its DNA of 5.05 Mbp has a GC content of 59.65%, with 77 tRNA genes and 3 rRNA genes. Its 4,909 putative PATRIC protein-coding genes include genes responsible for the degradation of dioxins and other xenobiotics and total consumption of benzoate.

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“…Whole-genome sequencing (WGS) was performed through Illumina NextSeq paired-end sequencing. The library preparation and 2 × 150-bp paired-end sequencing were performed using the Nextera XT Index kit v2 (FC-131-2001 to FC-131-2004) and a NextSeq 500 system (Illumina, San Diego, CA, USA) at Quadram Institute Bioscience (Norwich, UK) ( 4 ).…”
Section: Announcementmentioning
confidence: 99%
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“…Whole-genome sequencing (WGS) was performed through Illumina NextSeq paired-end sequencing. The library preparation and 2 × 150-bp paired-end sequencing were performed using the Nextera XT Index kit v2 (FC-131-2001 to FC-131-2004) and a NextSeq 500 system (Illumina, San Diego, CA, USA) at Quadram Institute Bioscience (Norwich, UK) ( 4 ).…”
Section: Announcementmentioning
confidence: 99%
“…WGS of B. thuringiensis SS2 revealed putative genes linked to the degradation of dioxin and other POPs similar to those of Serratia marcescens SSA1, which was isolated from the same burnt dump site. These genes include putative genes for total consumption of benzoate via hydroxylation and halogenated aromatic compound (biphenyl, toluene, xylene, styrene, benzoate, atrazine, and chloroethene) degradation, among others ( 4 ). B. thuringiensis SS2 contains putative lead-, cadmium-, zinc-, and mercury-transporting ATPase (EC 3.6.3.3 and EC 3.6.3.5) and copper-translocating P-type ATPase (EC 3.6.3.4) genes, suggesting heavy metal tolerance and resistance.…”
Section: Announcementmentioning
confidence: 99%