Draft Genome Sequence of Serratia proteamaculans MFPA44A14-05, a Model Organism for the Study of Meat and Seafood Spoilage

Fougy, Lysiane; Coeuret, Gwendoline; Champomier‐Vergès, Marie‐Christine; Chaillou, Stéphane

doi:10.1128/genomea.00491-17

Cited by 8 publications

(4 citation statements)

References 13 publications

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“…alvei is an ideal resistant-isolate candidate in any system. These traits can also be found in species of Shewanella [ 68 , 69 ], Serratia [ 70 , 71 ], Carnobacterium [ 72 ], Brochotrhix [ 73 , 74 ], and Kluyvera [ 75 , 76 ]. All of these bacteria can colonize multiple environments, and the ability to acquire resistance in any given environment represents a significant risk for human health.…”

Section: Discussionmentioning

confidence: 99%

Extended antibiotic treatment in salmon farms select multiresistant gut bacteria with a high prevalence of antibiotic resistance genes

et al. 2018

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The high use of antibiotics for the treatment of bacterial diseases is one of the main problems in the mass production of animal protein. Salmon farming in Chile is a clear example of the above statement, where more than 5,500 tonnes of antibiotics have been used over the last 10 years. This has caused a great impact both at the production level and on the environment; however, there are still few works in relation to it. In order to demonstrate the impact of the high use of antibiotics on fish gut microbiota, we have selected four salmon farms presenting a similar amount of fish of the Atlantic salmon species (Salmo salar), ranging from 4,500 to 6,000 tonnes. All of these farms used treatments with high doses of antibiotics. Thus, 15 healthy fish were selected and euthanised in order to isolate the bacteria resistant to the antibiotics oxytetracycline and florfenicol from the gut microbiota. In total, 47 bacterial isolates resistant to florfenicol and 44 resistant to oxytetracycline were isolated, among which isolates with Minimum Inhibitory Concentrations (MIC) exceeding 2048 μg/mL for florfenicol and 1024 μg/mL for oxytetracycline were found. In addition, another six different antibiotics were tested in order to demonstrate the multiresistance phenomenon. In this regard, six isolates of 91 showed elevated resistance values for the eight tested antibiotics, including florfenicol and oxytetracycline, were found. These bacteria were called “super-resistant” bacteria. This phenotypic resistance was verified at a genotypic level since most isolates showed antibiotic resistance genes (ARGs) to florfenicol and oxytetracycline. Specifically, 77% of antibiotic resistant bacteria showed at least one gene resistant to florfenicol and 89% showed at least one gene resistant to oxytetracycline. In the present study, it was demonstrated that the high use of the antibiotics florfenicol and oxytetracycline has, as a consequence, the selection of multiresistant bacteria in the gut microbiota of farmed fish of the Salmo salar species at the seawater stage. Also, the phenotypic resistance of these bacteria can be correlated with the presence of antibiotic resistance genes.

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Section: Discussionmentioning

confidence: 99%

Extended antibiotic treatment in salmon farms select multiresistant gut bacteria with a high prevalence of antibiotic resistance genes

et al. 2018

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“…S. proteamaculans was positively correlated with the TBARS value and b* value, indicating that S. proteamaculans might promote lipid oxidation. Fougy et al [33] have reported that S. proteamaculans can produce lipase to promote fat oxidation and produce yellowing.…”

Section: Pca and Correlation Analysismentioning

confidence: 99%

Prediction of specific spoilage organisms in smoked chicken legs with modified atmosphere packaging at 4 ℃ using multivariate statistical analysis

Wang,

Zhao,

Sui

et al. 2024

Food Science and Human Wellness

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The present study monitored bacterial succession, physicochemical properties, and volatile organic compounds (VOCs) changes in smoked chicken legs with modified atmosphere packaging (MAP, 60% CO 2 and 40% N 2 ) during a 25-day storage period at 4 ℃. After 15 days of storage, Serratia proteamaculans and Pseudomonas fragi became the predominant bacteria. Furthermore, physicochemical properties changed significantly, as evidenced by an increase in thiobarbituric acid reactive substances and b* (yellowness) value, and a decrease in hardness. A total of 65 VOCs were identified during storage. Correlation between bacterial succession and quality indicators (including VOCs and physicochemical properties) allowed the identification of 26 core dominant bacteria, including S. proteamaculans, Psychrobacter alimentarius, Pseudomonas putide, and Pseudomonas poae, which were positively related to spoilage VOCs (e.g., 1-octen-3-ol, 1-pentanol, and 3-methyl-1-butanol) and could be defined as specific spoilage organisms (SSOs). The results of this study provide a systematic approach to predict SSOs in smoked chicken legs during storage, which can also provide a basis for product safety.

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“…Bacillus spp. [9] , Brochothrix thermosphacta [17] , and Serratia [18][19][20] isolated from meat and meat products could secret lipase and protease, exacerbating meat spoilage.…”

Section: Activities Of Collagenase and Lipasementioning

confidence: 99%

Insight into the spoilage heterogeneity of meat-borne bacteria isolates with high-producing collagenase

HaoDong¹,

Shao²,

Zhang³

et al. 2024

Food Science and Human Wellness

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Chilled chicken is inevitably contaminated by microorganisms during slaughtering and processing, resulting in spoilage. Cutting parts of chilled chicken, especially wings, feet, and other skin-on products, are abundant in collagen, which may be the primary target for degradation by spoilage microorganisms. In this work, a total of 17 isolates of spoilage bacteria that could secrete both collagenase and lipase were determined by raw-chicken juice agar (RJA) method, and the results showed that 7 strains of Serratia, Aeromonas, and Pseudomonas could signifi cantly decompose the collagen ingredients. The gelatin zymography showed that Serratia liquefaciens (F5) and Pseudomonas saponiphila (G7) had apparent degradation bands around 50 kDa, and Aeromonas veronii (G8) and Aeromonas salmonicida (H8) had a band around 65 and 95 kDa, respectively. The lipase and collagenase activities were detected isolate-by-isolate, with F5 showing the highest collagenase activity. For spoilage ability on meat in situ, F5 performed strongest in spoilage ability, indicated by the total viable counts, total volatile basic nitrogen content, sensory scores, lipase, and collagenase activity. This study provides a theoretical basis for spoilage heterogeneity of strains with high-producing collagenase in meat.

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Draft Genome Sequence of Serratia proteamaculans MFPA44A14-05, a Model Organism for the Study of Meat and Seafood Spoilage

Cited by 8 publications

References 13 publications

Extended antibiotic treatment in salmon farms select multiresistant gut bacteria with a high prevalence of antibiotic resistance genes

Extended antibiotic treatment in salmon farms select multiresistant gut bacteria with a high prevalence of antibiotic resistance genes

Prediction of specific spoilage organisms in smoked chicken legs with modified atmosphere packaging at 4 ℃ using multivariate statistical analysis

Insight into the spoilage heterogeneity of meat-borne bacteria isolates with high-producing collagenase

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