Draft Genome Sequence of Streptomyces fradiae olg1-1, a Strain Resistant to Nitrone-Oligomycin

Vatlin, Aleksey A.; Bekker, Olga B.; Lysenkova, Lyudmila N.; Даниленко, В. Н.

doi:10.1128/genomea.01252-15

Cited by 2 publications

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“…In order to find the mutation that conferred resistance to nitrone‐oligomycin A of the mutant S. fradiae strain, we carried out a whole genome sequencing of S. fradiae ‐nitR + bld. The sequence of genome was deposited to NCBI GenBank under the accession number LGSP01000001 . Comparative Bioinformatic analysis to search for single nucleotide substitutions in the genome of the mutant strain as opposed to the wild‐type strain S. fradiae ATCC 19609 revealed six single nucleotide substitutions in different genes.…”

Section: Resultsmentioning

confidence: 99%

A functional study of the global transcriptional regulator PadR from a strain Streptomyces fradiae‐nitR+bld, resistant to nitrone‐oligomycin

et al. 2018

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We describe Streptomyces fradiae mechanisms of sensitivity to nitrone-oligomycin A, a derivative of oligomycin A. We obtained S. fradiae-nitR bld, a nitrone-oligomycin A resistant mutant with a «bald» phenotype. Comparative genomic analysis of the wild-type S. fradiae ATCC19609 and S. fradiae-nitR bld revealed a mutation in padR - a gene encoding a multifunction transcription regulator, which resulted in the amino acid replacement in a highly conserved DNA-binding domain. Bioinformatics genome analysis of S. fradiae ATCC19609 discovered a PadR binding site 13 bp upstream the start codon of the marR transcription factor gene. Induction of S. fradiaenitR bld and w.t. strains with nitrone-oligomycin A lead to a significant increase in expression level of the marR gene in the w.t. strain, but no change observed in mutant strain. We identified differences between DNA-protein interactions of the mutant and native PadR proteins with its putative binding site in S. fradiae ATCC19609. This allowed us to suggest that the padR gene, that harbored a single nucleotide mutation in the S. fradiaenitR bld strain, might be involved in the mechanism of resistance to nitrone-oligomycin A. We assume the participation of the transcriptional factorpadR in the formation of the bald phenotype.

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Section: Resultsmentioning

confidence: 99%

A functional study of the global transcriptional regulator PadR from a strain Streptomyces fradiae‐nitR+bld, resistant to nitrone‐oligomycin

et al. 2018

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Main trends in the design of semi-synthetic antibiotics of a new generation

Olsufyeva¹,

Yankovskaya²

2020

Russ. Chem. Rev.

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This review summarizes main advances achieved by Russian researchers in the synthesis and characterization of semi-synthetic antibiotics of a new generation in the period from 2004 to 2019. The following classes of compounds are considered as the basis for modification: polycyclic antibacterial glycopeptides of the vancomycin group, classical macrolides, antifungal polyene macrolides, the antitumour antibiotic olivomycin A, antitumour anthracyclines and broad-spectrum antibiotics, in particular, oligomycin A, heliomycin and some other. Main trends in the design of modern anti-infective and antitumour agents over this period are considered in relation to original natural antibiotics, which have been independently discovered by Russian researchers. It is shown that a new type of hybrid structures can, in principle, be synthesized based on glycopeptides, macrolides and other antibiotics, including heterodimers containing a new benzoxaborole pharmacophore. The review addresses the influence of the length of the spacer between two antibiotic molecules on the biological activity of hybrid structures. A combination of genetic engineering techniques and methods of organic synthesis is shown to be useful for the design of new potent antifungal antibiotics based on polyenes of the amphotericin B group. Many new semi-synthetic analogues exhibit important biological properties, such as a broad spectrum of activity and low toxicity. Emphasis is given to certain aspects related to investigation of a broad range of biological activity and mechanisms of action of new derivatives. The bibliography includes 101 references.

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Draft Genome Sequence of Streptomyces fradiae olg1-1, a Strain Resistant to Nitrone-Oligomycin

Abstract: We report a draft genome sequence of Streptomyces fradiae olg1-1, a mutant strain derived from the model object S. fradiae ATCC 19609, which is resistant to nitrone-oligomycin and has a mutation in the DNA-binding domain of a transcriptional regulator PadR.

Cited by 2 publications

References 12 publications

A functional study of the global transcriptional regulator PadR from a strain Streptomyces fradiae‐nitR+bld, resistant to nitrone‐oligomycin

A functional study of the global transcriptional regulator PadR from a strain Streptomyces fradiae‐nitR+bld, resistant to nitrone‐oligomycin

Main trends in the design of semi-synthetic antibiotics of a new generation

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