Draft Genome Sequence of the Antitrypanosomally Active Sponge-Associated Bacterium
            <i>Actinokineospora</i>
            sp. Strain EG49

Harjes, Janno; Ryu, Tae Woo; Abdelmohsen, Usama Ramadan; Moitinho‐Silva, Lucas; Horn, Hannes; Ravasi, Timothy; Hentschel, Ute

doi:10.1128/genomea.00160-14

Cited by 16 publications

(17 citation statements)

References 11 publications

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“…We found a peptide gene (accession number: ) which was analogous to propeptin on the genome of A. spheciospongiae (Harjes et al . ) by the blastp search (Fig. ).…”

Section: Resultsmentioning

confidence: 99%

“…SNA-115 (Kimura et al 1997), and the peptide sequence of propeptin was determined to be GYPWWDYRDLFGGHTFISP by MS/MS analysis. We found a peptide gene (accession number: EWC60872.1) which was analogous to propeptin on the genome of A. spheciospongiae (Harjes et al 2014) by the blastp search ( Fig. 1).…”

Section: Isolation Of Actinokineosinmentioning

confidence: 99%

“…Based on the genome mining approach, we found a new antibacterial peptide named actinokineosin from a rare actinomycete, Actinokineospora spheciospongiae, which was previously isolated from marine sponge tissue (Abdelmohsen et al 2010;Harjes et al 2014;Kampfer et al 2015). The structure was determined by mass spectrometry analyses on chemical and enzymatic degraded peptides.…”

Section: Introductionmentioning

confidence: 99%

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Isolation of a new antibacterial peptide actinokineosin fromActinokineospora spheciospongiaebased on genome mining

Takasaka

Kaweewan

Ohnishi‐Kameyama

et al. 2016

Lett Appl Microbiol

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“…We found a peptide gene (accession number: ) which was analogous to propeptin on the genome of A. spheciospongiae (Harjes et al . ) by the blastp search (Fig. ).…”

Section: Resultsmentioning

confidence: 99%

Section: Isolation Of Actinokineosinmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Isolation of a new antibacterial peptide actinokineosin fromActinokineospora spheciospongiaebased on genome mining

Takasaka

Kaweewan

Ohnishi‐Kameyama

et al. 2016

Lett Appl Microbiol

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“…The metabolomic and the genomic analyses of the strain showed its richness with diverse bioactive natural products. (Abdelmohsen et al , 2014; Harjes et al , 2014; Grkovic et al , 2014).…”

mentioning

confidence: 99%

Actinokineospora spheciospongiae sp. nov., isolated from the marine sponge Spheciospongia vagabunda

Kämpfer

Glaeser

Busse³

et al. 2015

International Journal of Systematic and Evolutionary Microbiology

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A Gram-staining-positive, aerobic organism, isolated from the Red Sea sponge Spheciospongia vagabunda was investigated to determine its taxonomic position. On the basis of results of 16S rRNA gene sequence analysis strain EG49T was most closely related to Actinokineospora cibodasensis and Actinokineospora baliensis (both 97.3 % similarity) and Actinokineospora diospyrosa and Actinokineospora auranticolor (both 97.0 % similarity). The 16S rRNA gene sequence similarity to all other species of the genus Actinokineospora was ,97.0 %. The quinone system of strain EG49 T contained the menaquinones MK-9(H 4 ) (47 %), MK-9(H 6 ) (27 %) and MK-9(H 2 ) (15 %) in major amounts. Minor amounts of MK-7(H 4 ) (2 %), MK-9(H 0 ) (1 %), MK-9(H 8 ) (3 %) and MK-10(H 4 ) (3 %) were detected as well in addition to MK-8(H 4 ), MK-8(H 6 ), MK-10(H 2 ) and MK-10(H 6 ) (all ,1 %). The diagnostic diamino acid of the peptidoglycan was mesodiaminopimelic acid. In the polar lipid profile, diphosphatidylglycerol, phosphatidylethanolamine and hydroxyphosphatidylethanolamine were predominant. Phosphatidylinositol-mannoside, two unidentified phospholipids and two glycoglipids as well as one aminoglycolipid, one aminolipid and one unidentified lipid were found in addition. The fatty acid profile was composed of mainly iso-branched fatty acids: iso-C 16 : 0 , iso-C 14 : 0 , iso-C 15 : 0 and iso-C 16 : 1 H. All these findings clearly supported the classification of the strain as representing a member of the genus Actinokineospora. In addition, the results of physiological and biochemical tests also allowed phenotypic differentiation of strain EG49 T from the most closely related species of the genus Actinokineospora. Strain EG49 T represents a novel species of the genus Actinokineospora, for which we propose the name Actinokineospora spheciospongiae sp. nov., with strain EG49as the type strain.The genus Actinokineospora was proposed by Hasegawa (1988), initially for motile, arthrospore-producing organisms of the class Actinomycetes. Labeda et al. (2010) recently emended the description of the genus, which now harbours also species for which the production of motile spores was not observed, and proposed the transfer of Amycolatopsis fastidiosa to this genus as Actinokineospora fastidiosa. The genus contains, at the time of writing, 13

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“…Actinokineospora is a rare endophytic actinomycetes which is found with cultureindependent on the leaves of medicinal plant Maytenus austroyunnanensis (Qin et al 2012). Actinokineospora has an ability to produce antimycoplasma (Hasegawa 1991) and antitrypanosomally actinosporin A and B (Harjes et al 2014;Abdelmonsen et al 2014). Analysis of soil actinomycetes community in Xinjiang, China using 16S rRNA based PCR-DGGE discovered species with a closedly related with Microbacterium (Zhang et al 2013).…”

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confidence: 99%

16S rRNA-based Metagenomic Analysis of Endophytic Actinomycetes Diversity from Tinospora crispa L. Miers

2015

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Endophytic actinomycetes associated with medicinal plants is very important as source of various bioactive compounds. The fact that more than 99% of microbes that exist in nature may have the potency but still unexplored. Published data regarding diversity of endophytic actinomycetes from Tinospora crispa is mainly based on a culturable approach. This paper describes the first reported data regarding metagenomic analysis on the diversity of endophytic actinomycetes from T.crispa based on 16S rRNA gene using PCR-DGGE. There were some similarities amongst endophytic actinomycetes found in stems, roots, and leaves with soil actinomycetes community in the rhizosphere of T. crispa. There were a total of 21 bands found from the DGGE analysis which were interpreted using Phoretix 1D software. Diversity of actinomycetes in the stems, leaves, roots were represented by 17, 16, and 14 bands, respectively. Whereas only 10 bands represented diversity of actinomycetes in the soil rhizosphere. The 12 dominant and or different bands with 180 bp in size were molecularly sequenced. The A4 and A9 bands have 95% and 86% similarities with Williamsia and Streptomyces, respectively. These similarities were less than 97% thus may indicate novel actinomycetes. The other 10 sequenced bands have closed similarity ranging from 97-100% and they were closely related to the genus Streptomyces, Microbacterium, Amycolatopsis, Actinomadura, Actinoplanes, Actinokineospora, Kibdelosporangium, Williamsia, and Kocuria. These findings indicate that diversity of actinomycetes can be found associated with T. crispa.Key words: 16S rRNA, DGGE, endophytic actinomycetes, metagenomic, Tinospora crispa Aktinomiset endofitik yang berasosiasi dengan tanaman obat berperan penting sebagai sumber berbagai senyawa bioaktif. Fakta mengindikasikan bahwa lebih dari 99% mikrob yang ada di alam diduga memiliki potensi, tetapi belum banyak dieksplorasi. Keragaman aktinomiset endofit yang telah dilaporkan masih terbatas pada pendekatan culturable. Data hasil penelitian ini merupakan informasi baru tentang keragaman aktinomiset endofit T. crispa yang dianalisis status metagenomiknya berdasarkan gen 16S rRNA menggunakan PCR-DGGE. Terdapat beberapa kesamaan antara aktinomiset endofit yang ditemukan di batang, akar, dan daun dengan komunitas aktinomiset tanah di rhizosfer T. crispa. Total pita yang ditemukan dari analisis DGGE yang menggunakan perangkat lunak Phoretix 1D yaitu sebanyak 21 pita. Keanekaragaman aktinomiset di batang, daun dan akar yaitu 17, 16 dan 14 pita, sementara hanya 10 pita ditemukan di tanah rhizosfer. Berdasarkan profil DGGE, diperoleh 12 pita yang dominan dengan panjang pita 180 bp yang kemudian disekuensing. Pita A4 dan A9 masing-masing memiliki kesamaan 95% dan 86% dengan Williamsia dan Streptomyces. Kesamaan sekuen tersebut kurang dari 97% sehingga diduga aktinomiset tersebut tergolong baru. Terdapat 10 pita lainnya yang memiliki kesamaan berkisar 97-100% dan berkekerabatan dekat dengan genus Streptomyces, Microbacterium, Amycolatopsis, Actinomadur...

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Draft Genome Sequence of the Antitrypanosomally Active Sponge-Associated Bacterium Actinokineospora sp. Strain EG49

Cited by 16 publications

References 11 publications

Isolation of a new antibacterial peptide actinokineosin fromActinokineospora spheciospongiaebased on genome mining

Isolation of a new antibacterial peptide actinokineosin fromActinokineospora spheciospongiaebased on genome mining

Actinokineospora spheciospongiae sp. nov., isolated from the marine sponge Spheciospongia vagabunda

16S rRNA-based Metagenomic Analysis of Endophytic Actinomycetes Diversity from Tinospora crispa L. Miers

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