Draft Genome Sequence of the Green Alga Scenedesmus acuminatus SAG 38.81

Astafyeva, Yekaterina; Alawi, Malik; Indenbirken, Daniela; Danso, Dominik; Grundhoff, Adam; Hanelt, Dieter; Streit, Wolfgang R.; Krohn, Ines

doi:10.1128/mra.01278-19

Cited by 8 publications

(6 citation statements)

References 12 publications

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“…A protein similar to the C. reinhardtii SULTR3 (Cre09.g401293), was not found in S. acutus. SULTR3 seems absent in Sphaeropleales, since we did not find a homologous sequence for this gene/protein, either in S. acutus or in data bank accession for Raphidocelis, Monoraphidium and in other Scenedesmus strains whose full genome sequencing has been recently obtained [32][33][34].…”

Section: Analysis Of Sulfate Transporter Genesmentioning

confidence: 75%

Role of Sulfate Transporters in Chromium Tolerance in Scenedesmus acutus M. (Sphaeropleales)

Ferrari

Cozza

Marieschi

et al. 2022

Plants

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Sulfur (S) is essential for the synthesis of important defense compounds and in the scavenging potential of oxidative stress, conferring increased capacity to cope with biotic and abiotic stresses. Chromate can induce a sort of S-starvation by competing for uptake with SO42− and causing a depletion of cellular reduced compounds, thus emphasizing the role of S-transporters in heavy-metal tolerance. In this work we analyzed the sulfate transporter system in the freshwater green algae Scenedesmus acutus, that proved to possess both H+/SO42− (SULTRs) and Na+/SO42− (SLTs) plasma membrane sulfate transporters and a chloroplast-envelope localized ABC-type holocomplex. We discuss the sulfate uptake system of S. acutus in comparison with other taxa, enlightening differences among the clade Sphaeropleales and Volvocales/Chlamydomonadales. To define the role of S transporters in chromium tolerance, we analyzed the expression of SULTRs and SULPs components of the chloroplast ABC transporter in two strains of S. acutus with different Cr(VI) sensitivity. Their differential expression in response to Cr(VI) exposure and S availability seems directly linked to Cr(VI) tolerance, confirming the role of sulfate uptake/assimilation pathways in the metal stress response. The SULTRs up-regulation, observed in both strains after S-starvation, may directly contribute to enhancing Cr-tolerance by limiting Cr(VI) uptake and increasing sulfur availability for the synthesis of sulfur-containing defense molecules.

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Section: Analysis Of Sulfate Transporter Genesmentioning

confidence: 75%

Role of Sulfate Transporters in Chromium Tolerance in Scenedesmus acutus M. (Sphaeropleales)

Ferrari

Cozza

Marieschi

et al. 2022

Plants

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“… *The chloroplast genome of SAG 38.81 was reconstructed from the genome assembly (GCA_902809745.2; Astafyeva et al., 2020 ). …”

Section: Resultsmentioning

confidence: 99%

“…From the sorted contigs, complete chloroplast genome sequences were reassembled using Geneious Prime (v2022.2). The chloroplast genome of SAG 38.81 was reconstructed from the genome assembly (GCA_902809745.2; Astafyeva et al., 2020 ). The prediction of protein-coding genes in the chloroplast genomes was performed by BLASTx search ( e -value cutoff=1.…”

Section: Methodsmentioning

confidence: 99%

Taxonomic reinvestigation of the genus Tetradesmus (Scenedesmaceae; Sphaeropleales) based on morphological characteristics and chloroplast genomes

Cho,

Lee

2024

Front. Plant Sci.

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The genus Tetradesmus (Scenedesmaceae; Sphaeropleales) comprises one of the most abundant green algae in freshwater environments. It includes morphologically diverse species that exhibit bundle-like, plane-arranged coenobia, and unicells, because several different Scenedesmus-like groups were integrated into this genus based on phylogenetic analysis. Nevertheless, there is no clear information regarding the phylogenetic relationship of Tetradesmus species, determined using several marker genes, because of low phylogenetic support and insufficient molecular data. Currently, genome information is available from diverse taxa, which could provide high-resolution evolutionary relationships. In particular, phylogenetic studies using chloroplast genomes demonstrated the potential to establish high-resolution phylogenetic relationships. However, only three chloroplast genomes are available from the genus Tetradesmus. In this study, we newly generated 9 chloroplast genomes from Tetradesmus and constructed a high-resolution phylogeny using a concatenated alignment of 69 chloroplast protein sequences. We also report one novel species (T. lancea), one novel variety (T. obliquus var. spiraformis), and two novel formae (T. dissociatus f. oviformis, T. obliquus f. rectilineare) within the genus Tetradesmus based on morphological characteristics (e.g., cellular arrangements and coenobial types) and genomic features (e.g., different exon–intron structures in chloroplast genomes). Moreover, we taxonomically reinvestigated the genus Tetradesmus based on these results. Altogether, our study can provide a comprehensive understanding of the taxonomic approaches for investigating this genus.

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“…Scenedesmus and Haematococcus species are Green Algae producing pluri-nuclear cells, with four to eight haploid nuclei, in the course of cell fission. Genomes of S. obliquus (Nag Dasgupta et al, 2018 ; Astafyeva et al, 2020 ) and H. pluvialis (Luo et al, 2019 ; Morimoto et al, 2020 ) are available. Transformation of S. obliquus via A. tumefasciens is feasible (Suttangkakul et al, 2019 ).…”

Section: Challenges In Microalgae Domesticationmentioning

confidence: 99%

Grand Challenges in Microalgae Domestication

Maréchal

2021

Front. Plant Sci.

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Draft Genome Sequence of the Green Alga Scenedesmus acuminatus SAG 38.81

Cited by 8 publications

References 12 publications

Role of Sulfate Transporters in Chromium Tolerance in Scenedesmus acutus M. (Sphaeropleales)

Role of Sulfate Transporters in Chromium Tolerance in Scenedesmus acutus M. (Sphaeropleales)

Taxonomic reinvestigation of the genus Tetradesmus (Scenedesmaceae; Sphaeropleales) based on morphological characteristics and chloroplast genomes

Grand Challenges in Microalgae Domestication

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