Draft Genome Sequence of the Agarase-Producing Sphingomonas sp. MCT13

D’Andrea, Marco Maria; Ciacci, Nagaia; Pilato, Vincenzo Di; Rossolini, Gian María; Thaller, María Cristina

doi:10.3389/fenvs.2017.00009

Cited by 1 publication

(2 citation statements)

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“…In addition more attention directed to optimize the enzyme production by Microbacterium spp., Bacillus subtilis , Dendryphiella arenaria & Agarivorans albus YKW-34 and then finding the finest condition to maximize the yields through OVAT or experimental design approaches as described by many researchers 15 – 18 , respectively. Microorganisms that hydrolyze agar can be employed as biological tackles for bioremediation and ecosystem self-cleaning 19 .…”

Section: Introductionmentioning

confidence: 99%

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Improvement of Bacillus subtilis PI agarase production, hydrolysate scavenging capability assessment, and saccharification of algal biomass for green ethanol generation

Goda,

Shalaby,

Soliman

2024

Sci Rep

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The goal of the current work was to optimize the growth parameters needed to manufacture agarase enzyme from a non-marine PI strain of Bacillus subtilis on an agar-based medium. Using Plackett–Burman design (PBD), nine process parameters were evaluated, and agar, peptone, and yeast-extract were identified as the most significant independent factors influencing agarase production with confidence levels more than 90%. To evaluate the optimal concentrations of the indicated process parameters on agarase production, the Box–Behnken design (BBD) was applied. After optimization, B. subtilis strain PI produced 119.8 U/ml of agarase, representing a 1.36-fold increase. In addition the agar hydrolysate fermented products contain the liberated oligosaccharide acts as strong antioxidant which has 62.4% scavenging activity. Also, the agarase yields increased (1141.12, 1350.253, 1684.854 and 1921.863 U/ml) after substitution the agar with algal biomass of Carolina officinalis at different concentrations (2, 5, 10 and 15%), respectively. After completing the saccharification process, the resulted hydrolysate was used to produce ethanol through fermentation with Pichia pastoris yeast strain as an economical method giving yields (6.68317, 7.09748, 7.75648 and 8.22332 mg/ml), that are higher than using yeast extract peptone dextrose (YPD) medium (4.461 mg/ml).

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Section: Introductionmentioning

confidence: 99%

“…NJ21 was isolated from antarctic marine environment 24 . Agarase producing bacteria were also isolated from laboratory wastes 18 , some factories 25 , a sewage treatment plant 26 and drainage wastewater 19 . Research on these non-marine, agar-degrading isolates may provide vision into the ecology of these kinds of microbes 23 .…”

Section: Introductionmentioning

confidence: 99%