Draft Genome Sequence of the Antarctic Psychrophilic Bacterium Pseudomonas syringae Strain Lz4W

Abstract

The psychrophilic bacterium Pseudomonas syringae strain Lz4W was isolated from soil samples from Antarctica to decipher the mechanisms of low-temperature adaptation. We report here the 4.982-Mb draft genome sequence of P. syringae Lz4W. This sequence will provide insights into the genomic basis of the psychrophilicity of this bacterium.

Genomics Insights into Pseudomonas sp. CG01: An Antarctic Cadmium-Resistant Strain Capable of Biosynthesizing CdS Nanoparticles Using Methionine as S-Source

“…GC01, Pseudomonas sp. Lz4W [ 50 ], and P. fragi P121 [ 51 ], suggesting that these strains belong to the same species. However, it is interesting that the P. fragi P121 strain did not group with the other two P. fragi strains included in the dataset, only sharing 85% identity with both strains.…”

“…Lz4W, Pseudomonas sp. L.10.10, and P. deceptionensis LMG25555; [ 50 , 52 , 54 ]. The P. aeruginosa strains were also the most divergent and ancestral branch among the set.…”

“…Lz4W, and P. sp. L.10.10 strains, inside a branch mostly composed of strains from cold environments [ 50 , 51 , 52 ]. A pan-genome analysis was performed to determine if the phenotypic differences could arise from the genotypic diversity between the Pseudomonas set.…”

The proteome of the outer membrane vesicles of an Antarctic bacterium Pseudomonas syringae Lz4W

“…This bacterial strain can grow between 0 °C and 30 °C with an optimum growth temperature of 22 °C [2] . It is a well studied bacterium in our laboratory whose genome sequence was recently published [3] . The bacterium P. syringae Lz4W was grown at 22 °C in Antarctic bacterial Medium (ABM) that contains 0.5% peptone and 0.2% yeast extract with aeration (by continuous shaking up to late stationary phase (OD 600 nm=1.1–1.2) for the preparation of OMVs.…”

“…The proteins from the Antarctic bacterium were identified earlier using different strategies, when the genome sequence is not available [9–12] . Recently, the draft genome sequence of the Antarctic bacterium P. syringae Lz4W shows that it contains 4450 entries and deposited at DDBJ/EMBL/GenBank under the accession number AOGS00000000 [3] . The MS/MS spectra of the multiply charged peptides were searched against this database of Pseudomonas Lz4W.…”