Draft Genome Sequence of Thermus scotoductus Strain K1, Isolated from a Geothermal Spring in Karvachar, Nagorno Karabakh

Saghatelyan, Ani; Poghosyan, Lianna; Panosyan, Hovik; Birkeland, Nils-Kåre

doi:10.1128/genomea.01346-15

Cited by 5 publications

(8 citation statements)

References 11 publications

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“…scotoductus SA‐01. This finding was expected, as according to earlier evaluations of the 16S rRNA gene sequences from these bacteria, they showed >99% similarity (Saghatelyan et al, 2015). Ts K1 DNA polymerase is relatively different from T .…”

Section: Discussionsupporting

confidence: 75%

“…The amino acid sequence comparison showed that this enzyme shares a high degree of similarity to DNA polymerase from T. scotoductus SA-01. This finding was expected, as according to earlier evaluations of the 16S rRNA gene sequences from these bacteria, they showed >99% similarity (Saghatelyan et al, 2015). TsK1 DNA polymerase is relatively different from T. aquaticus DNA polymerase, which might explain the differences in the behavior of this enzyme as discussed below.…”

Section: Discussionsupporting

confidence: 71%

“…The draft genome sequence of T. scotoductus K1 was deposited in the DBJ/EMBL/GenBank database under the RefSeq assembly accession no. GCF_001294665.1 (Saghatelyan et al, 2015). A phylogenetic tree depicting the evolutionary distance between TsK1 DNA polymerase (Accession no.…”

Section: Source Of Enzymementioning

confidence: 99%

“…Here, the expression, purification, and characterization of a recombinant DNA polymerase I from Thermus scotoductus strain K1 ( Ts K1 DNA polymerase) originating from a geothermal spring in Karvachar, Nagorno‐Karabakh (Saghatelyan et al, 2015) is described.…”

Section: Introductionmentioning

confidence: 99%

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Characteristics of DNA polymerase I from an extreme thermophile, Thermus scotoductus strain K1

et al. 2021

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Several native and engineered heat‐stable DNA polymerases from a variety of sources are used as powerful tools in different molecular techniques, including polymerase chain reaction, medical diagnostics, DNA sequencing, biological diversity assessments, and in vitro mutagenesis. The DNA polymerase from the extreme thermophile, Thermus scotoductus strain K1, (TsK1) was expressed in Escherichia coli, purified, and characterized. This enzyme belongs to a distinct phylogenetic clade, different from the commonly used DNA polymerase I enzymes, including those from Thermus aquaticus and Thermus thermophilus. The enzyme demonstrated an optimal temperature and pH value of 72–74°C and 9.0, respectively, and could efficiently amplify 2.5 kb DNA products. TsK1 DNA polymerase did not require additional K+ ions but it did need Mg2+ at 3–5 mM for optimal activity. It was stable for at least 1 h at 80°C, and its half‐life at 88 and 95°C was 30 and 15 min, respectively. Analysis of the mutation frequency in the amplified products demonstrated that the base insertion fidelity for this enzyme was significantly better than that of Taq DNA polymerase. These results suggest that TsK1 DNA polymerase could be useful in various molecular applications, including high‐temperature DNA polymerization.

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Section: Discussionsupporting

confidence: 75%

Section: Discussionsupporting

confidence: 71%

Section: Source Of Enzymementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characteristics of DNA polymerase I from an extreme thermophile, Thermus scotoductus strain K1

et al. 2021

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“…In total, more than 130 strains of 40 distinct species belonging to 22 different genera, namely Bacillus, Geobacillus, Parageobacillus, Anoxybacillus, Paenibacillus, Brevibacillus Aeribacillus, Ureibacillus, Thermoactinomyces, Sporosarcina, Thermus, Rhodobacter, Thiospirillum, Thiocapsa, Rhodopseudomonas, Methylocaldum, Desulfomicrobium, Desulfovibrio, Treponema, Arcobacter, Nitrospira , and Methanoculleus , have been isolated from the hot springs in Armenia and Nagorno-Karabakh. Among these isolates, many prospective strains have been selected for future studies to explore their potential in white (industrial), green (agricultural), and red (pharmaceutical) biotechnology [ 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 ].…”

Section: Microbiological Analysismentioning

confidence: 99%

Microbial Diversity of Terrestrial Geothermal Springs in Armenia and Nagorno-Karabakh: A Review

et al. 2021

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The microbial diversity of high-altitude geothermal springs has been recently assessed to explore their biotechnological potential. However, little is known regarding the microbiota of similar ecosystems located on the Armenian Highland. This review summarizes the known information on the microbiota of nine high-altitude mineralized geothermal springs (temperature range 25.8–70 °C and pH range 6.0–7.5) in Armenia and Nagorno-Karabakh. All these geothermal springs are at altitudes ranging from 960–2090 m above sea level and are located on the Alpide (Alpine–Himalayan) orogenic belt, a seismically active region. A mixed-cation mixed-anion composition, with total mineralization of 0.5 mg/L, has been identified for these thermal springs. The taxonomic diversity of hot spring microbiomes has been examined using culture-independent approaches, including denaturing gradient gel electrophoresis (DGGE), 16S rRNA gene library construction, 454 pyrosequencing, and Illumina HiSeq. The bacterial phyla Proteobacteria, Bacteroidetes, Cyanobacteria, and Firmicutes are the predominant life forms in the studied springs. Archaea mainly include the phyla Euryarchaeota, Crenarchaeota, and Thaumarchaeota, and comprise less than 1% of the prokaryotic community. Comparison of microbial diversity in springs from Karvachar with that described for other terrestrial hot springs revealed that Proteobacteria, Bacteroidetes, Actinobacteria, and Deinococcus–Thermus are the common bacterial groups in terrestrial hot springs. Contemporaneously, specific bacterial and archaeal taxa were observed in different springs. Evaluation of the carbon, sulfur, and nitrogen metabolism in these hot spring communities has revealed diversity in terms of metabolic activity. Temperature seems to be an important factor in shaping the microbial communities of these springs. Overall, the diversity and richness of the microbiota are negatively affected by increasing temperature. Other abiotic factors, including pH, mineralization, and geological history, also impact the structure and function of the microbial community. More than 130 bacterial and archaeal strains (Bacillus, Geobacillus, Parageobacillus, Anoxybacillus, Paenibacillus, Brevibacillus Aeribacillus, Ureibacillus, Thermoactinomyces, Sporosarcina, Thermus, Rhodobacter, Thiospirillum, Thiocapsa, Rhodopseudomonas, Methylocaldum, Desulfomicrobium, Desulfovibrio, Treponema, Arcobacter, Nitropspira, and Methanoculleus) have been reported, some of which may be representative of novel species (sharing 91–97% sequence identity with their closest matches in GenBank) and producers of thermozymes and biomolecules with potential biotechnological applications. Whole-genome shotgun sequencing of T. scotoductus K1, as well as of the potentially new Treponema sp. J25 and Anoxybacillus sp. K1, were performed. Most of the phyla identified by 16S rRNA were also identified using metagenomic approaches. Detailed characterization of thermophilic isolates indicate the potential of the studied springs as a source of biotechnologically valuable microbes and biomolecules.

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Microbial Diversity of Terrestrial Geothermal Springs in Lesser Caucasus

Panosyan

Margaryan

Poghosyan

et al. 2018

Extremophiles in Eurasian Ecosystems: Ecology, Diversity, and Applications

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Draft Genome Sequence of Thermus scotoductus Strain K1, Isolated from a Geothermal Spring in Karvachar, Nagorno Karabakh

Cited by 5 publications

References 11 publications

Characteristics of DNA polymerase I from an extreme thermophile, Thermus scotoductus strain K1

Characteristics of DNA polymerase I from an extreme thermophile, Thermus scotoductus strain K1

Microbial Diversity of Terrestrial Geothermal Springs in Armenia and Nagorno-Karabakh: A Review

Microbial Diversity of Terrestrial Geothermal Springs in Lesser Caucasus

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