Draft Genome Sequence of Vibrio mimicus Strain CAIM 602
            <sup>T</sup>

Guardiola-Avila, Iliana; Acedo-Félix, Evelia; Noriega-Orozco, Lorena; Yépiz-Plascencia, Glória; Sifuentes-Romero, Itzel; Gómez‐Gil, Bruno

doi:10.1128/genomea.00084-13

Cited by 5 publications

(11 citation statements)

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“…mimicus strains were sequenced using a semiconductor NGS platform (Ion Torrent Personal Genome Machine sequencer, Life Technologies) with a 316 chip at CIAD Mazatlán. The reads were assembled with the program Newbler ver.2.3 mapping against CAIM 602 T [ 31 ]. A genome-wide assembly and contig synteny was constructed with Mauve Genome Alignment software ver.…”

Section: Methodsmentioning

confidence: 99%

Molecular and Genomic Characterization of Vibrio mimicus Isolated from a Frozen Shrimp Processing Facility in Mexico

et al. 2016

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Vibrio mimicus is a gram-negative bacterium responsible for diseases in humans. Three strains of V. mimicus identified as V. mimicus 87, V. mimicus 92 and V. mimicus 93 were isolated from a shrimp processing facility in Guaymas, Sonora, Mexico. The strains were analyzed using several molecular techniques and according to the cluster analysis they were different, their similarities ranged between 51.3% and 71.6%. ERIC-PCR and RAPD (vmh390R) were the most discriminatory molecular techniques for the differentiation of these strains. The complete genomes of two strains (V. mimicus 87, renamed as CAIM 1882, and V. mimicus 92, renamed as CAIM 1883) were sequenced. The sizes of the genomes were 3.9 Mb in both strains, with 2.8 Mb in ChI and 1.1 Mb in ChII. A 12.7% difference was found in the proteome content (BLAST matrix). Several virulence genes were detected (e.g. capsular polysaccharide, an accessory colonization factor and genes involved in quorum-sensing) which were classified in 16 categories. Variations in the gene content between these genomes were observed, mainly in proteins and virulence genes (e.g., hemagglutinin, mobile elements and membrane proteins). According to these results, both strains were different, even when they came from the same source, giving an insight of the diversity of V. mimicus. The identification of various virulence genes, including a not previously reported V. mimicus gene (acfD) in ChI in all sequenced strains, supports the pathogenic potential of this species. Further analysis will help to fully understand their potential virulence, environmental impact and evolution.

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Section: Methodsmentioning

confidence: 99%

Molecular and Genomic Characterization of Vibrio mimicus Isolated from a Frozen Shrimp Processing Facility in Mexico

et al. 2016

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“…In addition, Vibrio mimicus , the closely related Vibrio species of V. cholerae , can also share important virulence factors such as enterotoxins or haemolysins, suggesting contemporary horizontal transfer of the CTX and VPI prophages (Boyd, Moyer, Shi, & Waldor, ). It can be isolated from a large number of environmental sources such as fresh, brackish and sea water (Campos et al., ; Guardiola‐Avila et al., ). V. mimicus has been linked to foodborne illnesses in recent years causing sporadic cases of diarrhoea with cholera‐like signs (Guardiola‐Avila et al., ).…”

Section: Introductionmentioning

confidence: 99%

Virulence‐associated factors in Vibrio cholerae non‐O1/non‐O139 and V. mimicus strains isolated in ornamental fish species

Zago

Zambon

Civettini

et al. 2017

Journal of Fish Diseases

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During recent decades, ornamental fish have proven to be one of the fastest growing categories of pets in Europe. In this framework, we evaluated both the potential pathogenic and zoonotic risks caused by 53 Vibrio cholerae non-O1/non-O139 and a Vibrio mimicus strain isolated from ornamental fish species mostly originating from South-East Asia countries between 2000 and 2015 in Italy. All the strains were firstly identified at species level by biochemical, phylogenetic and mass spectrometry (matrix-assisted laser desorption ionization time of flight) methods, and then studied to reveal the presence of the main virulence and colonization-associated factors, as ctxA, ace, zot, stn/sto, toxR, rtxA, hlyA and tcpA by multiplex and single endpoint PCR assays. Findings showed that 21 of 54 strains harboured at least one virulence factor with a predominance for the toxR , rtxA and hlyAET genotype. Interestingly, the V. mimicus strain harboured the colonization factor and the CTX prophage receptor, tcpA, indicating the ability to capture and integrate it in its genome increasing its pathogenicity. Although these enterotoxins can sporadically cause gastroenteritis, the results highlight their probable involvement in causing severe implications for public health, suggesting the need for an European microbiological monitoring.

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“…Nine genomes of V. mimicus were analyzed in this study. The genomes of V. mimicus CAIM-602 T (Vm602, PRJNA179483) [ 7 ], V. mimicus CAIM-1882 (Vm1882, PRJNA219179), V. mimicus CAIM-1883 (Vm1883, PRJNA219181) [ 20 ], and V. mimicus ATCC-33654 (Vm 33654, PRJNA231624) were sequenced by our research group, as previously reported [ 7 , 20 ]. The remaining five genomes sequences were obtained from the database of the National Center for Biotechnology Information (NCBI): V. mimicus MB451 (Vm451, PRJNA40509), V. mimicus VM223 (Vm223, PRJNA40483), V. mimicus VM603 (Vm603, PRJNA40241), V. mimicus VM573 (Vm573, PRJNA40243), and V. mimicus SX-4 (Vm SX4, PRJNA47421) [ 6 , 10 , 14 ].…”

Section: Methodsmentioning

confidence: 99%

“…Vibrio mimicus has been recognized as an emergent pathogen in human diseases, and it has been isolated from cases of gastroenteritis, ear infections, and severe cholera-like diarrhoea, as well as from several marine products, aquatic plants, sediments, and water (marine, brackish, and freshwater) [ 3 , 4 , 5 ]. V. mimicus strains encode a wide variety of virulence factors, such as he, hemagglutinins, pili, metalloproteases, enterotoxins, and siderophores, which are mediated by several different mechanisms [ 5 , 6 , 7 , 8 ]. Moreover, studies of comparative genomics of Vibrio species have shown that genetic exchange among Vibrionaceae family species is a continuous process [ 6 , 9 , 10 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Core and Accessory Genome Analysis of Vibrio mimicus

et al. 2021

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Vibrio mimicus is an emerging pathogen, mainly associated with contaminated seafood consumption. However, little is known about its evolution, biodiversity, and pathogenic potential. This study analyzes the pan-, core, and accessory genomes of nine V. mimicus strains. The core genome yielded 2424 genes in chromosome I (ChI) and 822 genes in chromosome II (ChII), with an accessory genome comprising an average of 10.9% of the whole genome for ChI and 29% for ChII. Core genome phylogenetic trees were obtained, and V. mimicus ATCC-33654 strain was the closest to the outgroup in both chromosomes. Additionally, a phylogenetic study of eight conserved genes (ftsZ, gapA, gyrB, topA, rpoA, recA, mreB, and pyrH), including Vibrio cholerae, Vibrio parilis, Vibrio metoecus, and Vibrio caribbenthicus, clearly showed clade differentiation. The main virulence genes found in ChI corresponded with type I secretion proteins, extracellular components, flagellar proteins, and potential regulators, while, in ChII, the main categories were type-I secretion proteins, chemotaxis proteins, and antibiotic resistance proteins. The accessory genome was characterized by the presence of mobile elements and toxin encoding genes in both chromosomes. Based on the genome atlas, it was possible to characterize differential regions between strains. The pan-genome of V. mimicus encompassed 3539 genes for ChI and 2355 genes for ChII. These results give us an insight into the virulence and gene content of V. mimicus, as well as constitute the first approach to its diversity.

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Draft Genome Sequence of Vibrio mimicus Strain CAIM 602 ^T

Abstract: Vibrio mimicus is a Gram-negative bacterium associated with gastrointestinal diseases in humans around the world. We report the complete genome sequence of the Vibrio mimicus strain CAIM 602T (CDC1721-77, LMG 7896T, ATCC 33653T).

Cited by 5 publications

References 9 publications

Molecular and Genomic Characterization of Vibrio mimicus Isolated from a Frozen Shrimp Processing Facility in Mexico

Molecular and Genomic Characterization of Vibrio mimicus Isolated from a Frozen Shrimp Processing Facility in Mexico

Virulence‐associated factors in Vibrio cholerae non‐O1/non‐O139 and V. mimicus strains isolated in ornamental fish species

Core and Accessory Genome Analysis of Vibrio mimicus

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Draft Genome Sequence of Vibrio mimicus Strain CAIM 602 T

Abstract: Vibrio mimicus is a Gram-negative bacterium associated with gastrointestinal diseases in humans around the world. We report the complete genome sequence of the Vibrio mimicus strain CAIM 602T (CDC1721-77, LMG 7896T, ATCC 33653T).

Cited by 5 publications

References 9 publications

Molecular and Genomic Characterization of Vibrio mimicus Isolated from a Frozen Shrimp Processing Facility in Mexico

Molecular and Genomic Characterization of Vibrio mimicus Isolated from a Frozen Shrimp Processing Facility in Mexico

Virulence‐associated factors in Vibrio cholerae non‐O1/non‐O139 and V. mimicus strains isolated in ornamental fish species

Core and Accessory Genome Analysis of Vibrio mimicus

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Draft Genome Sequence of Vibrio mimicus Strain CAIM 602 ^T