Draft Genome Sequence of White Spot Syndrome Virus Isolated from Cultured Litopenaeus vannamei in Mexico

Abstract

The first genome sequence of a Mexican white spot syndrome virus is presented here. White spot syndrome is a shrimp pandemic virus that has devastated production in Mexico for more than 10 years. The availability of this genome will greatly aid epidemiological studies worldwide, contributing to the molecular diagnostic and disease prevention in shrimp farming.

Complete Genome Sequence of White Spot Syndrome Virus Isolated from Indian White Prawn ( Fenneropenaeus indicus ) in Egypt

“…The genome sequences of 12 WSSV isolates are available, including those of four isolates from China (GenBank accession no. AF332093, KT995472, KT995470, and KT995471) (5, 6) and one isolate each from Thailand (AF369029) (7), Taiwan (AF440570) (8), Korea (JX515788) (9), India (MG702567) (10), Brazil (MF784752) (11), Mexico (KU216744) (12), Australia (MF768985) (13), and Ecuador (MH090824) (14). The genome size of these isolates varies from 284 to 309 kb, with 147 to 526 predicted open reading frames (ORFs).…”

A new strain of white spot syndrome virus affecting Litopenaeus vannamei in Indian shrimp farms

“…After deletion, the size of the genome of WSSV‐NS was estimated about 279,797 bp which is the smallest size of genome when compared to other strains such as WSSV‐TW (307,287 bp), WSSV‐CN (305,119 bp), WSSV‐KR (295,884), WSSV‐MX (293,183 bp) and WSSV‐TH (292,967). We observed a total deletion of 27,490, 25,322, 16,087, 13,170 and 13,386 bp relative to WSSV‐TW, WSSV‐CN, WSSV‐KR, WSSV‐TH and WSSV‐MX, respectively (Chai et al., ; Rodriguez‐Anaya et al., ; Tsai et al., ; Van Hulten et al., ; Yang et al., ).…”

“…() reported a deletion of 11,453 bp in the region ORF 23/24 relative to WSSV‐TW in Brazilian WSSV isolates. Recently, complete genome sequence of Mexican WSSV isolate was published and sequence analysis revealed the lack of ORFs 122 and 123 as in WSSV‐KR and VP35 gene was also found to be missing as observed in the WSSV‐TH (Rodriguez‐Anaya et al., ). It is noted that most genomic deletions which occurred in the ORFs 23 and 24 of WSSV isolates were reported from the Asian region.…”

“…Even though treatment for WSSV infection is not well established, the genome architecture is well known. Five complete genome sequences for WSSV collected from different parts of the world are presently available in public domain, which include WSSV from Taiwan with 307,287 nucleotides (WSSV‐TW AF440570) (Tsai et al., ), from China with 305,107 nucleotides (WSSV‐CN AF332093) (Yang et al., ), from Thailand with 292,967 nucleotides (WSSV‐TH AF369029) (Van Hulten et al., ), from Korea with 295,884 nucleotides (WSSV‐KR JX515788) (Chai, Yoon, Lee, Kim, & Choi, ) and from Mexico with 293,183 nucleotides (WSSV‐MX08 KU 216744) (Rodriguez‐Anaya et al., ).They are highly conserved with a sequence similarity of 97%–99% among all five genomes. Over the years, several deletion sites have been identified in different isolates (Dieu et al., ; Marks, van Duijse, Zuidema, van Hulten, & Vlak, ; Muller, Andrade, Tang‐Nelson, Marques, & Lightner, ; Oakey & Smith, ; Pradeep, Shekar, Karunasagar, & Karunasagar, ; Rodriguez‐Anaya et al., ).…”

Draft Genome Sequence of a White Spot Syndrome Virus Isolate Obtained in Ecuador

“…A total of 162 coding sequences (CDS) were predicted. The genomic architectures were compared with strains from China ( 3 ), Thailand ( 10 ), South Korea ( 4 ), and Mexico ( 11 ) using Mauve ( 12 ). A complete synteny among the assembled genomes was observed.…”