Draft Genome Sequences of Propionibacterium acnes Type Strain ATCC6919 and Antibiotic-Resistant Strain HL411PA1

Liu, Jared; Cheng, Alison Lai Fong; Bangayan, Nathanael J.; Barnard, Emma; Curd, Emily; Craft, Noah; Li, Huiying

doi:10.1128/genomea.00740-14

Cited by 19 publications

(20 citation statements)

References 13 publications

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“…A PCR product spanning the two ends with the predicted size (~735 bp) and sequence was amplified from all the phages tested, suggesting that the phage DNA can exist in a circular form mediated by the overhangs at the ends (Supplementary Figure S6A). To demonstrate the pseudolysogenic properties of P. acnes phages, we infected P. acnes strain ATCC6919, a clade IA-1 strain (Liu et al, 2014), with five different phages (PHL060L00, PHL112N00, PHL037M02, PHL073M02 and PHL114L00). Following the formation of plaques by each phage, regrowth of the bacteria was observed starting in the center of the plaque regions.…”

Section: Diversity Of P Acnes Phages In the Human Skinmentioning

confidence: 99%

The diversity and host interactions of Propionibacterium acnes bacteriophages on human skin

et al. 2015

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The viral population, including bacteriophages, is an important component of the human microbiota, yet is poorly understood. We aim to determine whether bacteriophages modulate the composition of the bacterial populations, thus potentially playing a role in health or disease. We investigated the diversity and host interactions of the bacteriophages of Propionibacterium acnes, a major human skin commensal implicated in acne pathogenesis. By sequencing 48 P. acnes phages isolated from acne patients and healthy individuals and by analyzing the P. acnes phage populations in healthy skin metagenomes, we revealed that P. acnes phage populations in the skin microbial community are often dominated by one strain. We also found phage strains shared among both related and unrelated individuals, suggesting that a pool of common phages exists in the human population and that transmission of phages may occur between individuals. To better understand the bacterium-phage interactions in the skin microbiota, we determined the outcomes of 74 genetically defined Propionibacterium strains challenged by 15 sequenced phages. Depending on the Propionibacterium lineage, phage infection can result in lysis, pseudolysogeny, or resistance. In type II P. acnes strains, we found that encoding matching clustered regularly interspaced short palindromic repeat spacers is insufficient to confer phage resistance. Overall, our findings suggest that the prey-predator relationship between bacteria and phages may have a role in modulating the composition of the microbiota. Our study also suggests that the microbiome structure of an individual may be an important factor in the design of phage-based therapy.

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Section: Diversity Of P Acnes Phages In the Human Skinmentioning

confidence: 99%

The diversity and host interactions of Propionibacterium acnes bacteriophages on human skin

et al. 2015

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“…Certain subtypes may be better adapted at thriving in the deep tissues and causing clinical infection than others (13,14,16). Also, some subtypes may be more strongly associated with clinical markers of infection, such as hemolysis, and antibiotic resistance (16)(17)(18)(19)(20)(21)(22). Sequencing of deep tissue samples has recently become a candidate for potential rapid diagnosis (23) and for identifying virulent and antibiotic-resistant strains (14).…”

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confidence: 99%

Cutibacterium acnes Isolates from Deep Tissue Specimens Retrieved during Revision Shoulder Arthroplasty: Similar Colony Morphology Does Not Indicate Clonality

2020

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Cutibacterium acnes is the most common bacterium associated with periprosthetic shoulder infections. Sequencing of C. acnes has been proposed as a potential rapid diagnostic tool and a method of determining subtypes associated with pathogenicity and antibiotic resistance patterns. When multiple deep samples from the same surgery are culture positive for the same species and the isolates show the same culture phenotype, it is typically assumed that these isolates are clonal. However, it is well-known that C. acnes is not clonal on the skin of most individuals. We hypothesized that the C. acnes bacteria recovered at the time of revision shoulder arthroplasty would often represent more than one subtype, and we tested this hypothesis in this work. For patients undergoing revision shoulder arthroplasty, multiple samples from the surgical field were taken. For those patients with multiple samples that were culture positive for C. acnes, isolates from each sample were subjected to full genome sequencing. Of 11 patients, 5 (45%) had different subtypes of C. acnes within the deep tissues even though the colony morphology was similar. One patient had four subtypes in the deep tissues, while four patients had two different subtypes. Up to four different subtypes of C. acnes were observed in the deep tissues of a single patient. Clonality of C. acnes isolates from deep specimens from a potential periprosthetic shoulder infection cannot be assumed. Sequence-based characterization of virulence and antibiotic resistance may require testing of multiple deep specimens.

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“…Cationic AMPs, like P5, show broad-spectrum antimicrobial activity, kill target cells rapidly, and are active against antibiotic resistant and clinically relevant pathogens, including P . acnes [ 3 , 29 , 30 ]. It is generally accepted that the mechanism of action of cationic AMPs involves their direct interaction with the negatively charged bacterial membranes, which causes an increase in membrane permeability that rapidly leads to cell death [ 16 ].…”

Section: Discussionmentioning

confidence: 99%

“…acnes directly stimulate the production of TNF-α and IL-8 via TLR expression [ 37 ]. Recent studies have shown that cationic peptides can inhibit the inflammation induced by bacteria, perhaps by altering cytokine production [ 30 – 32 ]. For example, several synthetic cationic AMPs have been shown to block LPS- and LTA-stimulated production of pro-inflammatory cytokines by macrophages [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

Suppression of Propionibacterium acnes Infection and the Associated Inflammatory Response by the Antimicrobial Peptide P5 in Mice

et al. 2015

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The cutaneous inflammation associated with acne vulgaris is caused by the anaerobic bacterium Propionibacterium acnes through activation of the innate immune system in the skin. Current standard treatments for acne have limitations that include adverse effects and poor efficacy in many patients, making development of a more effective therapy highly desirable. In the present study, we demonstrate the protective effects of a novel customized α-helical cationic peptide, P5, against P. acnes-induced inflammatory responses in vitro and in vivo. Application of P5 significantly reduced expression of two inflammatory cytokines IL-8 and TNF-α in P. acnes-treated primary human keratinocytes, where P5 appeared to act in part by binding to bacterial lipoteichoic acid, thereby suppressing TLR2-to-NF-κB signaling. In addition, in a mouse model of acne vulgaris, P5 exerted both anti-inflammatory and antimicrobial effects against P. acnes, but exerted no cytotoxic effects against skin cells. These results demonstrate that P5, and perhaps other cationic antimicrobial peptides, offer the unique ability to reduce numbers P. acnes cells in the skin and to inhibit the inflammation they trigger. This suggests these peptides could potentially be used to effectively treat acne without adversely affecting the skin.

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Draft Genome Sequences of Propionibacterium acnes Type Strain ATCC6919 and Antibiotic-Resistant Strain HL411PA1

Abstract: Propionibacterium acnes is a major skin commensal and is associated with acne vulgaris, the most common skin disease. Here we report the draft genome sequences of two P. acnes strains, the type strain ATCC6919 and an antibiotic-resistant strain, HL411PA1.

Cited by 19 publications

References 13 publications

The diversity and host interactions of Propionibacterium acnes bacteriophages on human skin

The diversity and host interactions of Propionibacterium acnes bacteriophages on human skin

Cutibacterium acnes Isolates from Deep Tissue Specimens Retrieved during Revision Shoulder Arthroplasty: Similar Colony Morphology Does Not Indicate Clonality

Suppression of Propionibacterium acnes Infection and the Associated Inflammatory Response by the Antimicrobial Peptide P5 in Mice

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